Abstract For many migraine patients, triptan therapy provides complete pain relief in some attacks but not others. Here, we tested whether the success of is hindered presence cutaneous allodynia (pain resulting from a nonnoxious stimulus to normal skin), phenomenon previously described develop gradually during course attack more than 70% patients. We studied patients repeatedly on three visits clinic: absence (baseline), within first hour one attack, or at 4 hours onset another attack....

Attacks of migraine with aura represent a phenomenon in which abnormal neuronal activity the cortex produces sensory disturbances (aura) some 20-40 min before onset headache. The purpose this study was to determine whether cortical spreading depression (CSD)--an event believed underlie visual aura--can give rise activation nociceptors that innervate meninges--an set off CSD induced anesthetized male rats by stimulation electrical pulses, pin prick, or KCl; single-unit meningeal monitored...

Abstract Objective Focal somatic pain can evolve into widespread hypersensitivity to nonpainful and painful skin stimuli (allodynia hyperalgesia, respectively). We hypothesized that transformation of headache whole‐body allodynia/hyperalgesia during a migraine attack is mediated by sensitization thalamic neurons process converging sensory impulses from the cranial meninges extracephalic skin. Methods Extracephalic allodynia was assessed using single unit recording trigeminovascular in rats...

In 459 migraine attacks, information provided to patients (from negative neutral positive) modified placebo and medication outcomes in a progressive fashion.

Cortical spreading depression (CSD) has long been implicated in migraine attacks that begin with visual aura. Having shown a wave of CSD can trigger long-lasting activation meningeal nociceptors--the first-order neurons the trigeminovascular pathway thought to underlie headache--we now report activate central spinal trigeminal nucleus (C1-2).

Intracranial headaches such as that of migraine are generally accepted to be mediated by prolonged activation meningeal nociceptors but the mechanisms responsible for nociceptor poorly understood. In this study, we examined hypothesis can activated locally through a neuroimmune interaction with resident mast cells, granulated immune cells densely populate dura mater. Using in vivo electrophysiological single unit recording rat observed degranulation dural using intraperitoneal administration...

Abstract We have shown that the development of cutaneous allodynia (exaggerated skin sensitivity) during migraine is detrimental to anti‐migraine action 5HT IB/ID receptor agonists known triptans. Because a manifestation sensitization central trigeminovascular neurons, we examined whether triptan treatment can intercept such before its initiation or after establishment in our rat model for induced by intracranial pain. Single‐unit recordings were obtained from spinal trigeminal neurons...

Triptans are 5HT 1B/1D receptor agonists commonly prescribed for migraine headache. Although originally designed to constrict dilated intracranial blood vessels, the mechanism and site of action by which triptans abort pain remain unknown. We showed recently that sensitization peripheral central trigeminovascular neurons plays an important role in pathophysiology pain. Here we examined whether drug sumatriptan can prevent and/or suppress using single-unit recording our animal model found...

Migraine sufferers frequently testify that their headache feels as if the calvarial bones are deformed, crushed, or broken (Jakubowski et al. [2006] Pain 125:286-295). This has lead us to postulate supplied by sensory fibers. We studied innervation of calvaria in coronal and horizontal sections whole-head preparations postnatal adult mice, via immunostaining peripherin (a marker thinly myelinated unmyelinated fibers) calcitonin gene-related peptide (CGRP; a more typical nerve fibers). In...

This study identifies massive axonal arbors of trigeminovascular (dura-sensitive) thalamic neurons in multiple cortical areas and proposes a novel framework for conceptualizing migraine headache its associated symptoms. Individual dura-sensitive identified characterized electrophysiologically first-order higher-order relay nuclei were juxtacellularly filled with an anterograde tracer that labeled their cell bodies processes. First-order located the ventral posteromedial nucleus projected...

GnRH regulates gonadotropin biosynthesis and release in the anterior pituitary via specific receptors. Although extrapituitary expression action of have been shown some species, human it is not clear whether has a peripheral action. In this study we sought to determine ovary expresses receptor (GnRHR) messenger ribonucleic acid (mRNA). Ovarian tissues from 11 women (32-61 yr old) granulosa-lutein (GL) cells purified follicular aspirates 51 undergoing oocyte retrieval for vitro fertilization...

Migraine headache is routinely managed using medications that abort attacks as they occur. An alternative approach to migraine management based on prophylactic reduce attack frequency. One has been local intramuscular injections of Botulinum Toxin Type A (BTX-A). Here, we explored for neurological markers might distinguish patients who benefit from BTX-A treatment (100 units divided into 21 sites across pericranial and neck muscles). Responders non-responders were compared prospectively...

A common complaint among pain patients is that they lose their appetite. These accounts are anecdotal, however, and the neural mechanism underlying pain-induced loss of appetite remains unknown. In this study, we documented occurrence in under migraine attack investigated neuronal substrate anorexia our animal model intracranial pain. We found during humans coincided strongly with onset duration head 32/39 cases, brief noxious stimulation dura conscious rats produced a transient suppression...

<b>Objective: </b> The gradual development of cutaneous allodynia during the course a migraine attack is commonly detected by quantitative sensory testing (QST) in migraineurs seeking secondary and tertiary medical help. In this study, authors developed questionnaire that tested recollection patients on their skin sensitivity past attacks. <b>Methods: devised series questions regarding posed them to 89 when they were free (Visit 1). To validate recollections, determined patients9 pain...

Serine-threonine protein kinases in the ribosomal S6 kinase (rsk or p90rsk) family have been implicated as signaling intermediates cellular response to several growth factors. To investigate molecular diversity of human p90rsk isoforms, mixed degenerate oligonucleotide polymerase chain reaction was used isolate partial rsk cDNAs (1.1 kb). Three closely related were obtained (HU-1, HU-2, HU-3). These are encoded by separate genes based on DNA sequence and distinct patterns seen with genomic...

Using quantitative sensory testing (QST), we found that many migraineurs seeking secondary and tertiary care exhibit cutaneous allodynia whenever they undergo a migraine attack, but not interictally (i.e. between attacks). When such patients were questionned in the clinic about symptoms of skin sensitivity past attacks, 76% them 'correctly' classified either as allodynic (>or=1 symptom) or non-allodynic (zero symptoms) line with QST analysis. In this study, if documented any one symptom...

The rat preoptic area-anterior hypothalamic continuum (POA-AH) contains about 400–800 neurons that express the decapeptide GnRH and 56-amino-acid GnRHassociated peptide. Originating from olfactory placode, these migrate establish their final distribution connections in POA-AH several days before birth. aim of present study was to examine whether biosynthesis mRNA encoding precursor (proGnRH) common GnRH-associated peptide undergoes postnatal changes corresponding development sexual...