A5051129335- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Advanced Numerical Analysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Nuclear Materials and Properties
- Single-cell and spatial transcriptomics
- Fusion materials and technologies
- Computer Graphics and Visualization Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Analytical chemistry methods development
- Analytical Chemistry and Sensors
- Biosensors and Analytical Detection
- Cellular Mechanics and Interactions
- Hydrogen embrittlement and corrosion behaviors in metals
- Manufacturing Process and Optimization
- Muscle Physiology and Disorders
- Nuclear materials and radiation effects
- Barrier Structure and Function Studies
- Traditional Chinese Medicine Analysis
- Neurogenetic and Muscular Disorders Research
- Photodynamic Therapy Research Studies
- Retinal Diseases and Treatments
- Spinal Cord Injury Research
- Microwave Dielectric Ceramics Synthesis
Guangzhou Institutes of Biomedicine and Health
2025
Guangzhou Medical University
2025
Chinese Academy of Sciences
2025
Chongqing Medical University
2022-2024
University of Science and Technology Beijing
2020-2024
State Power Investment Corporation (China)
2023
Wuhan National Laboratory for Optoelectronics
2017-2022
Huazhong University of Science and Technology
2017-2022
Optics Technology (United States)
2022
Organisation de Coopération et de Développement Economiques
2021
We developed an advanced clearing method with superior fluorescence-preserving capability for 3D imaging of whole organs.
Abstract Tissue optical clearing techniques have provided important tools for large‐volume imaging. Aqueous‐based methods are known good fluorescence preservation and scalable size maintenance, but limited by long incubation time, insufficient performance, or requirements specialized devices. Additionally, few compatible with widely used lipophilic dyes while maintaining high performance. Here, to address these issues, m‐xylylenediamine (MXDA) is firstly introduced into tissue develop a...
In this paper, we proposed an automatic crack detection method based on deep convolutional neural network -U-Net [4]. Unlike existing machine learning methods, can process image as a whole without patchifying, thanks to the encoder-decoder structure of U-Net. The segmentation result is output from whole, instead aggregation neighborhood patches. addition, new cost function distance transform introduced assign pixel-level weight according minimal ground truth segmentation. experiments, test...
Recently, a variety of tissue optical clearing techniques have been developed to reduce light scattering for imaging deeper and three-dimensional reconstruction structures. Combined with diverse labeling methods, these methods significantly promoted the development neuroscience. Each them has its own characteristics certain advantages disadvantages. Though there are some comparison results, covered limited evaluation indices lack uniformity, which made it difficult select best-fit protocol...
Tissue optical clearing enables imaging deeper in large volumes with high-resolution. Clear T2 is a relatively rapid method no use of solvents or detergents, hence poses great advantage on preservation diverse fluorescent labels. However, this suffers from insufficient tissue transparency, especially for adult mouse brain blocks. In work, we develop and versatile based , termed RTF (Rapid Triethanolamine Formamide), aiming better capability. The results show that can not only efficiently...
Abstract Rapid 3D imaging of entire organs and organisms at cellular resolution is a recurring challenge in life science. Here we report on computational light-sheet microscopy able to achieve minute-timescale high-resolution mapping macro-scale organs. Through combining dual-side confocally-scanned Bessel illumination which provides thinner-and-wider optical sectioning deep tissues, with content-aware compressed sensing (CACS) computation pipeline further improves the contrast based single...
Motor endplates (MEPs) are the important interfaces between peripheral nerves and muscle fibers. Investigation of spatial distribution MEPs could help us better understand neuromuscular functional activities improve diagnosis therapy related diseases. Methods: Fluorescent α-bungarotoxin was injected to label motor in whole-mount skeletal muscles, tissue optical clearing combined with light-sheet microscopy used investigate in-muscle nerve branches different muscles wild-type transgenic...
Tissue optical clearing techniques provide alternative approaches for imaging large-volume specimens. uDISCO, an organic-solvent-based method, stands out from the enormous array of available methods by achieving whole-brain with high transparency, size reduction and fluorescence preservation. In this study, we aimed to modify uDISCO protocol achieve better preservation thereby further improve its quality. First, determined optimal pH value optimized termed 'a-uDISCO' (alkaline pH-based...
The photodynamic (PD) effect has been reported to be efficient for the opening of blood-brain barrier (BBB), which provides a new informative platform developing perspective strategies towards brain disease therapy and drug delivery. However, this method is usually performed via craniotomy due high scattering turbid skull. In work, we employed newly-developed optical clearing skull window investigating non-invasive PD-induced BBB weight molecules 100-nm fluid-phase liposomes containing...
Highlights•Isolation of gpEpiSCs resembling E10.5–E11.5 epiblasts from embryos•gpEpiSC self-renewal depends on FGF2, ACTIVIN A, and WNT signaling inhibition•gpEpiSCs show transcriptional features the primed pluripotent stateSummaryGuinea pigs are valuable models for human disease research, yet lack established stem cell lines has limited their utility. In this study, we isolate characterize guinea pig epiblast cells (gpEpiSCs) post-implantation embryos. These differentiate into three germ...
Abstract The recent integration of light‐sheet microscopy and tissue‐clearing has facilitated an important alternative to conventional histological imaging approaches. However, the in toto cellular mapping neural circuits throughout intact mouse brain remains highly challenging, requiring complicated mechanical stitching, suffering from anisotropic resolution insufficient for high‐quality reconstruction 3D. Here, use a multiangle‐resolved subvoxel selective plane illumination microscope...
We present a three-dimensional (3D) isotropic imaging of mouse brain using light-sheet fluorescent microscopy (LSFM) in conjunction with multi-view computation. Unlike common single view LSFM is used for imaging, the tissue 3D imaged under eight views our study, by home-built selective plane illumination (SPIM). An output image containing complete structural information as well significantly improved resolution ([Formula: see text]4 times) are then computed based on these data, bead-guided...
Large vessel recanalization in ischemic stroke does not always go along with tissue reperfusion, a phenomenon called "no-reflow". However, knowledge of the mechanism no-reflow is limited because identifying microvascular obstruction across cortex and subcortex both clinical experimental models challenging. In this study, we developed smart three-dimensional recognition pipeline for during post-ischemia reperfusion to examine underlying no-reflow.
Background Photodynamic therapy (PDT), a minimally invasive therapeutic tool, has been an important option for post‐surgical treatment of malignant gliomas (MGs) in both adult and young patients. Recent studies have shown that PDT can also open the blood‐brain barrier (BBB). However, there are no optimized parameters patients at different ages. To determine whether age differences effects on BBB, we studied PDT‐related BBB opening through optical clearing skull window healthy 4‐ 8‐week‐old...
Abstract The development of the neuromuscular system, including muscle growth and intramuscular neural development, in addition to central nervous system maturation, determines motor ability improvement. Motor occurs asynchronously from cephalic caudal. However, whether structural different muscles is heterochronic unclear. Here, based on characteristics behavior postnatal mice, we examined 3D features by combining tissue clearing with optical imaging techniques. Quantitative analyses data...
A dense and uniform Cr coating was fabricated on the zirconium alloys fuel claddings by radio frequency (RF) magnetron sputtering to improve mechanical anti-oxidation properties under 1200 °C steam environment. The phase composition micro macro morphologies of specimens were investigated X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), scanning electron microscope (SEM) optical-microtopography (OM) analyses, high-temperature oxidation behavior evaluated at environment for 3000...
Knowledge regarding the relationship between muscles and corresponding motor neurons would allow therapeutic genes to transport into specific spinal cord segments. Retrograde tracing technique by targeting endplate (MEP), a highly specialized structure that offers direct access neurons, has been used elucidate connectivity skeletal innervating neuron pools. However, current injection strategies mainly based on blind or local MEP region might lead an underestimation of number due uneven...
Three-dimensional visualization of the innervation in skeletal muscles is helpful for understanding morphological structure and function. iDISCO, a whole-mount immunolabeling clearing technique, provides valuable tool volume imaging intramuscular nerve fibers but suffers from nonspecific staining caused by anti-mouse secondary antibody when using murine primary antibody. We developed modified iDISCO method introducing pretreatment ScaleCUBIC-1 reagent, termed m-iDISCO. The m-iDISCO could...
The tissue optical clearing technique plays an important role in three-dimensional (3D) visualization of large tissues. As a typical solvent-based method, 3DISCO can achieve the highest level transparency with favorable speed. However, cannot deal residual blood within tissues, leading to brownness or redness after clearing, thus greatly influencing and image quality due strong absorption blood. To address this problem, we proposed optimized method by introducing CUBIC-L solution combined...
Significance: The recently reported solvent-based optical clearing method FDISCO can preserve various fluorescent signals very well. However, the strict low-temperature storage condition of is not conducive to long-time or repetitive imaging usually conducted at room temperature. Therefore, it important solve contradiction between fluorescence preservation and condition. Aim: We develop a modified method, termed FDISCO+, change from low temperature Approach: Two alternative antioxidants were...