A5025933862- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Photoreceptor and optogenetics research
- Botulinum Toxin and Related Neurological Disorders
- Neurogenesis and neuroplasticity mechanisms
- Muscle activation and electromyography studies
- 14-3-3 protein interactions
- Transcranial Magnetic Stimulation Studies
- Corneal surgery and disorders
- Cardiomyopathy and Myosin Studies
- Nerve injury and regeneration
- Cellular Mechanics and Interactions
- Neurological disorders and treatments
- Nanoplatforms for cancer theranostics
- Advanced Electron Microscopy Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Spinal Cord Injury Research
- Analytical Chemistry and Sensors
Wuhan National Laboratory for Optoelectronics
2015-2021
Huazhong University of Science and Technology
2015-2021
We developed an advanced clearing method with superior fluorescence-preserving capability for 3D imaging of whole organs.
Abstract Tissue optical clearing techniques have provided important tools for large‐volume imaging. Aqueous‐based methods are known good fluorescence preservation and scalable size maintenance, but limited by long incubation time, insufficient performance, or requirements specialized devices. Additionally, few compatible with widely used lipophilic dyes while maintaining high performance. Here, to address these issues, m‐xylylenediamine (MXDA) is firstly introduced into tissue develop a...
Motor endplates (MEPs) are the important interfaces between peripheral nerves and muscle fibers. Investigation of spatial distribution MEPs could help us better understand neuromuscular functional activities improve diagnosis therapy related diseases. Methods: Fluorescent α-bungarotoxin was injected to label motor in whole-mount skeletal muscles, tissue optical clearing combined with light-sheet microscopy used investigate in-muscle nerve branches different muscles wild-type transgenic...
Tissue optical clearing technique shows a great potential for neural imaging with high resolution, especially connectomics in brain. The passive clarity (PACT) is relative simple method based on incubation, which has advantage tissue transparency, fluorescence preservation and immunostaining compatibility blocks. However, this suffers from long processing time. Previous studies indicated that increasing temperature can speed up the clearing. In work, we aim to systematacially quantitatively...
The developed optical clearing methods show great potential for imaging of large-volume tissues, but these present some nonnegligible limitations such as complexity implementation and long incubation times. In this study, we tried to screen out rapid agents by means molecular dynamical simulation experimental demonstration. According the sugar sugar-alcohol, further evaluated improvement in efficacy mouse brain samples, depth, fluorescence preservation, linear deformation. results showed...
Abstract Introduction Motor endplates are composed of a motor neuron terminal and muscle fiber distributed in skeletal muscle, causing contraction. However, traditional endplate staining methods limited to the observation partial muscle. The procedure was time‐consuming due strict incubation conditions, usually provided unsatisfactory results. We explored novel method label rapidly by vivo injection fluorescent α ‐bungarotoxin. Methods Fifty‐two mice were randomly divided into two groups, an...
The tissue optical clearing technique plays an important role in three-dimensional (3D) visualization of large tissues. As a typical solvent-based method, 3DISCO can achieve the highest level transparency with favorable speed. However, cannot deal residual blood within tissues, leading to brownness or redness after clearing, thus greatly influencing and image quality due strong absorption blood. To address this problem, we proposed optimized method by introducing CUBIC-L solution combined...
A simple tissue optical clearing method was developed based on Clear, which can clear Neonatal (P2) whole-brain within one day. The efficacy of adult mice brain samples is much more than that Clear and Clear. does not affect the EGFP fluorescence.
The quantification and distribution characteristics of spinal motor neurons play important roles in the study cord peripheral nerve injury repair. In most research, sole retrograde labeling each or muscle could not simultaneously obtain distributions different neuron subpopulations. Therefore, it did allow mapping spatial relationships columns for disclosing functional relationship branches. Here, we combined multiple labeling, optical clearing, imaging three-dimensional (3D) visualization...
The development of various optical clearing methods provides a great potential for imaging entire central nervous system by combining with multiple-labelling and microscopic techniques. These had made certain contributions respective weaknesses, including tissue deformation, fluorescence quenching, execution complexity antibody penetration limitation that makes immunostaining blocks difficult. passive clarity technique (PACT) bypasses those problems clears the samples simple implementation,...
The rise of various optical clearing methods provides a great potential for imaging deep inside tissues by combining with multiple-labelling and microscopic techniques. However, these had certain weaknesses, including toxicity reagents, tissue deformation, fluorescence quenching, complexity implementation. In this study, we developed simple rapid agents improving depth observing the process obtaining images. After brain slices were immersed sorbitol, sucrose fructose 1 or 2 min,...
The emergence of various optical clearing methods provides a great potential for imaging deep inside tissues by combining with multiple-labelling and microscopic techniques. They were generally developed specific demand thus presented some non-negligible limitations such as long incubation time, tissue deformation, fluorescence quenching, incompatibility immunostaining or lipophilic tracers. In this study, we rapid versatile method, termed ReagentTF, fluorescent samples. This method can not...
Various tissue optical clearing techniques have emerged for large-volume imaging. Most of them suffer from long processing time. Here, we introduced rapid methods thin and thick brain blocks, respectively.
Tissue optical clearing technique plays an important role in three-dimensional (3D) visualization of intact organs or rodent bodies. As a typical organic-solvent based method, 3DISCO renders the turbid tissues transparent by strategy minimizing light scattering, and possesses advantages high transparency substantial size reduction, which could facilitate imaging large-volume tissues. However, for heme-rich tissues, such as spleen, liver, embryo clinical biopsy samples, tissue image quality...
Tissue optical clearing techniques have provided important tools for large-volume imaging. Aqueous-based methods are known good fluorescence preservation and scalable size maintenance, but limited by either long incubation time, or insufficient performance, requirements specialized devices. Additionally, due to the use of high concentration organic solvents detergents, few compatible with lipophilic dyes while maintaining performance. To address these issues, we developed a rapid, highly...
Tissue optical clearing techniques have provided important tools for large-volume imaging. Here, m-xylylenediamine (MXDA) is firstly introduced into tissue and used to develop a rapid, highly efficient aqueous method with robust lipophilic dyes compatibility, termed MXDA-based Aqueous Clearing System (MACS). MACS can render whole adult brains transparent within 2.5 d also applicable other intact organs. Meanwhile, possesses ideal compatibility multiple probes, especially dyes. achieves...