A5072248139- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- AI in cancer detection
- Immune Cell Function and Interaction
- Image and Signal Denoising Methods
- Image Processing Techniques and Applications
- Viral Infections and Immunology Research
- Nanofabrication and Lithography Techniques
- Photorefractive and Nonlinear Optics
- Digital Holography and Microscopy
- Advanced Optical Sensing Technologies
- Advanced Data Compression Techniques
- Hepatitis C virus research
- Phase-change materials and chalcogenides
- CAR-T cell therapy research
- Medical Image Segmentation Techniques
- Optical Coatings and Gratings
Huazhong University of Science and Technology
2011-2024
Wuhan National Laboratory for Optoelectronics
2022-2024
Tongji Hospital
2011
Abstract Chalcogenide glasses are widely applied in the fields of infrared optics and nonlinear optics, which has spurred extensive research into their processing fabrication, particularly for grating structures. We propose a novel method fabricating structures chalcogenide glass thin films utilizing photobleaching effect via direct exposure. Two kinds structure were fabricated perform well diffraction effect. This is easy to implement cost-effective, making it ideal large-scale production...
Clarification of the cytotoxic function T cells is crucial for understanding human immune responses and immunotherapy procedures. Here, we report a high-throughput Bessel oblique plane microscopy (HBOPM) platform capable 3D live imaging phenotyping chimeric antigen receptor (CAR)-modified T-cell cytotoxicity against cancer cells. The HBOPM has following characteristics: an isotropic subcellular resolution 320 nm, large-scale scouting over 400 interacting cell pairs, long-term observation...
With the rapid development in advanced imaging techniques, massive image data have been acquired for various biomedical applications, posing significant challenges to their efficient storage, transmission, and sharing. Classical model- or learning-based compression algorithms are optimized specific dimensional neglect semantic redundancy multidimensional data, resulting limited performance. Here, we propose a Semantic based Implicit Neural Compression guided with Saliency map (SINCS)...
<title>Abstract</title> Despite offering rapid and gentle 3D imaging of biological samples, the revolution by light-sheet fluorescence microscopy faces challenges due to optical constraints limited geometry flexibility, hindering its widespread application. Here we present Two-beam Interference Microscopy (TIM), which sweeps interference pattern two symmetric laser sheets achieve horizontal plane illumination orthogonal wide-field detection for first time using a single objective. The...
To explore the mechanisms of a novel potassium channel gene named KCTD9 (potassium tetramerization domain containing 9) in model fulminant viral hepatitis induced by murine virus 3 (MHV-3).78 BALB/cJ mice(6 male) were randomly and equally assigned to two groups, group MHV3 its control. 75 C3H/HeJ female mice done into 39 for chronic MHV3, 36 Various samples including spleen, liver lymphocytes from groups controls examined expression real time quantitative PCR Immunohistochemistry....
Rapid 3D imaging of meso- or macro-scale organs and organisms at sub-micron resolution is challenging in life sciences. We present a static light-sheet microscopy that can achieve sequences high speed up to 100 fps. Our method utilizes double-ring light modulation module the illumination optics path produce low sidelobe Bessel light-sheet, providing hardware benefits for high-throughput imaging. In our study tumor microenvironment human lymphoma tissue, we achieved triple-color achromatic...
The tradeoff between spatial-temporal resolutions and phototoxicity prevents fast 3D super-resolution imaging from living cells, hindering the reveal of subcellular interactions. Key challenges lie in quests to optimal excitation efficient super-resolution. Here, we present a double-ring modulated SPIM design toward an ultrathin uniform light sheet with low side lobes. combination IDDR our isotropic divide-stages-to-process (ID) networks allows live-cell at 100 nm resolution volume rate up...