A5100709184- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Autism Spectrum Disorder Research
- MicroRNA in disease regulation
- Marine and coastal plant biology
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Marine and coastal ecosystems
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- RNA regulation and disease
- Cell death mechanisms and regulation
- Single-cell and spatial transcriptomics
- Neural dynamics and brain function
- Nerve injury and regeneration
- Meat and Animal Product Quality
University of Wisconsin–Madison
2016-2025
Chinese Academy of Sciences
2004-2025
Sichuan University
2014-2025
State Key Laboratory of Biotherapy
2012-2025
Tongji University
2023-2025
Dalian Medical University
2011-2025
Second Affiliated Hospital of Dalian Medical University
2017-2025
Tongji Hospital
2024-2025
Yantai University
2023-2025
Sun Yat-sen University
2025
Phosphorylation of CREB (cyclic AMP [cAMP]- response element [CRE]-binding protein) by cAMP-dependent protein kinase (PKA) leads to the activation many promoters containing CREs. In neurons and other cell types, phosphorylation CRE-containing can occur in elevated intracellular Ca2+. cultured cells that normally lack this Ca2+ responsiveness, we confer Ca(2+)-mediated a promoter introducing an expression vector for Ca2+/calmodulin-dependent type IV (CaMKIV). Activation could also be mediated...
Both microRNAs (miRNAs) and epigenetic regulation have important functions in stem cell biology, although the interactions between these two pathways are not well understood. Here, we show that MeCP2, a DNA methyl-CpG–binding protein, can epigenetically regulate specific miRNAs adult neural cells (aNSCs). MeCP2-mediated of one such miRNA, miR-137, involves coregulation by Sox2, core transcription factor cells. miR-137 modulates proliferation differentiation aNSCs vitro vivo. Overexpression...
Abstract The maturation of young neurons is regulated by complex mechanisms and dysregulation this process frequently found in neurodevepmental disorders. MicroRNAs have been implicated several steps neuronal including dendritic axonal growth, spine development, synaptogenesis. We demonstrate that one brain-enriched microRNA, miR-137, has a significant role regulating maturation. Overexpression miR-137 inhibits morphogenesis, phenotypic maturation, development both brain cultured primary...
The protective role of sodium glucose cotransporter 2 (SGLT2) inhibitors in renal outcomes has been revealed by large cardiovascular outcome trials among patients with type diabetes. However, the effect SGLT2 on lupus nephritis (LN) and its underlying mechanisms remain unknown.We applied empagliflozin treatment to lupus-prone MRL/lpr mice explore potential inhibitors. An knockout monoclonal podocyte cell line was generated using CRISPR/Cas9 system examine cellular molecular mechanisms.In...
Probing how human neural networks operate is hindered by the lack of reliable tissues amenable to dynamic functional assessment circuits. We developed a 3D bioprinting platform assemble with defined cell types in desired dimension using commercial bioprinter. The printed neuronal progenitors differentiate into neurons and form circuits within between tissue layers specificity weeks, evidenced cortical-to-striatal projection, spontaneous synaptic currents, response excitation. Printed...
DNA methylation-mediated epigenetic regulation plays critical roles in regulating mammalian gene expression, but its role normal brain function is not clear. Methyl-CpG binding protein 1 (MBD1), a member of the methylated DNA-binding family, has been shown to bind promoters and facilitate transcriptional repression vitro. Here we report generation analysis MBD1-/- mice. mice had no detectable developmental defects appeared healthy throughout life. However, found that neural stem cells...
Multipotent neural stem/progenitor cells (NSPCs) can be isolated from many regions of the adult central nervous system (CNS), yet neurogenesis is restricted to hippocampus and subventricular zone in vivo. Identification molecular cues that modulate NSPC fate choice a prerequisite for their therapeutic applications. Previously, we demonstrated primary astrocytes with higher neuroplasticity, such as newborn spinal cord, promoted neuronal differentiation NSPCs, whereas nonneurogenic region cord...
The hippocampus consists of a series cytoarchitecturally discrete subregions that can be distinguished from one another on the basis morphology, connectivity, and electrophysiological properties. To understand molecular underpinnings for these differences, DNA microarrays were used to find genes predicted enriched in CA1, CA3, dentate gyrus, >100 subsequently analyzed using situ hybridization obtain cellular-level localization their transcripts. most striking commonality among resulting...
Fragile X syndrome (FXS), the most common form of inherited mental retardation, is caused by loss functional fragile retardation protein (FMRP). FMRP an RNA–binding that can regulate translation specific mRNAs. Adult neurogenesis, a process considered important for neuroplasticity and memory, regulated at multiple molecular levels. In this study, we investigated whether Fmrp deficiency affects adult neurogenesis. We show in mouse model syndrome, neurogenesis indeed altered. The increases...
Abstract Adult neurogenesis is regulated by both intrinsic programs and extrinsic stimuli. The enhanced proliferation of adult neural stem/progenitor cells (aNPCs) in the subventricular zone migration neuroblasts toward ischemic region brains present a unique challenge as well an opportunity to understand molecular mechanisms underlying cue-induced neurogenic responses. Matrix metalloproteinases (MMPs) are family proteinases known play role extracellular matrix remodeling cell migration....
Abstract The hippocampus consists of distinct anatomic regions that have been demonstrated to different biological functions. To explore the molecular differences between hippocampal subregions, we performed transcriptional profiling analysis by using DNA microarray technology. cRNA derived from CA1, CA3, and dentate gyrus spinal cord was hybridized Affymetrix high‐density oligo arrays. This systematic approach revealed sets genes were expressed specifically in subregions corresponding...
Fragile X syndrome (FXS), a common inherited form of intellectual disability with learning deficits, results from loss fragile mental retardation protein (FMRP). Despite extensive research, treatment options for FXS remain limited. Since FMRP is known to play an important role in adult hippocampal neurogenesis and hippocampus-dependent regulates the neural stem cell fate through translational regulation glycogen synthase kinase 3β (GSK3β), we investigated effects GSK3β inhibitor, SB216763,...