A5100425015- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic Syndromes and Imprinting
- DNA Repair Mechanisms
- Genetic Neurodegenerative Diseases
- RNA Interference and Gene Delivery
- Neurogenesis and neuroplasticity mechanisms
- Viral Infections and Vectors
- melanin and skin pigmentation
- Resilience and Mental Health
- Parkinson's Disease Mechanisms and Treatments
- Plant Disease Management Techniques
Emory University
2016-2025
Shandong Agricultural University
2024
Chongqing University of Arts and Sciences
2024
Chongqing Three Gorges University
2024
Sichuan Agricultural University
2023-2024
Nanjing Medical University
2024
Jiangsu Province Hospital
2024
Hospital of Hebei Province
2022
Washington Center
2022
Nankai University
2018
Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, hold tremendous promise for both basic research clinical applications. To date, a serious knowledge gap remains our understanding of DNA repair mechanisms early embryos, efficiency potential off-target effects using technologies CRISPR/Cas9 pre-implantation embryos. In this...
Both microRNAs (miRNAs) and epigenetic regulation have important functions in stem cell biology, although the interactions between these two pathways are not well understood. Here, we show that MeCP2, a DNA methyl-CpG–binding protein, can epigenetically regulate specific miRNAs adult neural cells (aNSCs). MeCP2-mediated of one such miRNA, miR-137, involves coregulation by Sox2, core transcription factor cells. miR-137 modulates proliferation differentiation aNSCs vitro vivo. Overexpression...
Covalent modification of DNA distinguishes cellular identities and is crucial for regulating the pluripotency differentiation embryonic stem (ES) cells. The recent demonstration that 5-methylcytosine (5-mC) may be further modified to 5-hydroxymethylcytosine (5-hmC) in ES cells has revealed a novel regulatory paradigm modulate epigenetic landscape pluripotency. To understand role 5-hmC epigenomic pluripotent cells, here we profile genome-wide distribution correlate it with genomic profiles 11...
Neuronal intranuclear inclusion disease (NIID) is a slowly progressing neurodegenerative characterized by eosinophilic inclusions in the nervous system and multiple visceral organs. The clinical manifestation of NIID varies widely, both familial sporadic cases have been reported. Here we performed genetic linkage analysis mapped locus to 1p13.3-q23.1; however, whole-exome sequencing revealed no potential disease-causing mutations. We then long-read genome identified large GGC repeat...
N6-methyladenosine (m6A) is the most prevalent internal modification of mammalian messenger RNAs (mRNAs) and long non-coding RNAs. The biological functions this reversible RNA can be interpreted by cytoplasmic nuclear 'm6A reader' proteins to fine-tune gene expression, such as mRNA degradation translation initiation. Here we profiled transcriptome-wide m6A sites in adult mouse cerebral cortex, underscoring that a widespread epitranscriptomic brain. Interestingly, targets fragile X mental...
Chemical modifications on DNA molecules, such as 5-methylcytosine and 5-hydroxymethylcytosine, play important roles in the mammalian brain. A novel adenine modification, N(6)-methyladenine (6mA), has recently been found cells. However, presence function(s) of 6mA brain remain unclear. Here we demonstrate dynamics mouse response to environmental stress. We find that overall levels are significantly elevated upon Genome-wide transcriptome profiling reveal an inverse association between dynamic...
Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs development function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed Asian ZIKVC, African ZIKVM, dengue (DENV). In contrast the robust global transcriptome changes induced by DENV, a more selective larger impact on expression genes involved in DNA replication repair. While overall profiles are similar, but not induces...
The nucleus is a unique organelle that contains essential genetic materials in chromosome territories. interchromatin space composed of nuclear subcompartments, which are defined by several distinctive bodies believed to be factories DNA or RNA processing and sites transcriptional and/or posttranscriptional regulation. In this paper, we performed genome-wide microscopy-based screening for proteins form foci characterized their localizations using markers known bodies. total, identified 325...
PARP inhibitors (PARPis) are being used in patients with BRCA1/2 mutations. However, doubly deficient BRCA1 −/− 53BP1 cells or tumors become resistant to PARPis. Since its known downstream effectors, PTIP and RIF1 (RAP1-interacting factor 1 homolog), lack enzymatic activities directly implicated DNA repair, we decided further explore the 53BP1-dependent pathway. In this study, uncovered a nuclease, Artemis, as PTIP-binding protein. Loss of Artemis restores PARPi resistance BRCA1-deficient...
Significance DNA methylation is essential for mammalian development. This modification nearly completely erased and reestablished in early embryos, but specific classes of elements escape such genome-wide changes via unknown mechanisms. In this study, we identified a likely factor responsible lack turnover on large fraction sequences. By focusing mouse embryonic stem cells depleted de novo methyltransferases, which exhibit widespread hypomethylation with the exception particular loci, show...
Abstract Emerging evidence suggests that active DNA demethylation machinery plays important epigenetic roles in mammalian adult neurogenesis; however, the precise molecular mechanisms and critical functional players of this process remain largely unexplored. Ten–eleven translocation (Tet) proteins convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) its downstream derivatives. Here we show 5hmC is elevated during differentiation neural stem cells (aNSCs), Tet2 primarily...
Abstract DICER1 is an enzyme that generates mature microRNAs (miRNAs), which regulate gene expression post-transcriptionally in brain and other tissues involved synaptic maturation plasticity. Here, through genome-wide differential survey of post-traumatic stress disorder (PTSD) with comorbid depression (PTSD&Dep), we find blood significantly reduced cases versus controls, replicate this two independent cohorts. Our follow-up studies lower associated increased amygdala activation to...
5-hydroxymethylcytosine (5hmC) is enriched in brain and has been recognized as an important DNA modification. However, the roles of 5hmC its writers, ten-eleven translocation (Tet) proteins, stress-induced response have yet to be elucidated. Here, we show that chronic restraint stress (CRS) induced depression-like behavior mice resulted a reduction prefrontal cortex (PFC). We found loss Tet1 (Tet1 KO) led resistance CRS, whereas Tet2 (Tet2 increased susceptibility CRS. Genome-wide profiling...
In Drosophila melanogaster the reciprocal “Ping-Pong” cycle of PIWI-interacting RNA (piRNA)–directed cleavage catalyzed by endonuclease (or “Slicer”) activities PIWI proteins Aubergine (Aub) and Argonaute3 (AGO3) has been proposed to expand secondary piRNA population. However, role AGO3/Aub Slicer activity in amplification remains be explored. We show that AGO3 is essential for inhibits homotypic Aub:Aub Ping-Pong process a Slicer-independent manner. also find expression an mutant causes...