A5100604738- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Genetics and Neurodevelopmental Disorders
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
- Natural product bioactivities and synthesis
- Genomics and Phylogenetic Studies
- Traditional Chinese Medicine Studies
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Technology and Security Systems
- Essential Oils and Antimicrobial Activity
- Liver Disease Diagnosis and Treatment
- Protein Hydrolysis and Bioactive Peptides
- Immune cells in cancer
- Cell Image Analysis Techniques
- Ginseng Biological Effects and Applications
- Hemoglobinopathies and Related Disorders
- Academic Publishing and Open Access
- Salivary Gland Tumors Diagnosis and Treatment
- Polysaccharides Composition and Applications
- Cholinesterase and Neurodegenerative Diseases
- Research, Science, and Academia
Zunyi Medical University
2025
ShanghaiTech University
2020-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Dalian Polytechnic University
2024
Children's Hospital of Zhejiang University
2023
Shanghai Institute of Nutrition and Health
2023
University of Chinese Academy of Sciences
2023
Fujian University of Traditional Chinese Medicine
2013-2019
Emory University
2013-2018
Shanghai Institutes for Biological Sciences
2017
DNA methylation is a key epigenetic mechanism in brain aging and Alzheimer's disease (AD). The newly discovered 5-hydroxymethylcytosine mediates demethylation, highly abundant the brain, dynamically regulated by life experiences. However, little known about its genome-wide patterns potential role AD.Using capture followed high-throughput sequencing, we studied distribution of at specific genomic loci human AD identified differentially hydroxymethylated regions (DhMRs) associated with...
Gene–environment interactions mediated at the epigenetic level may provide an initial step in delivering appropriate response to environmental changes. 5-Hydroxymethylcytosine (5hmC), a DNA base derived from 5-methylcytosine (5mC), accounts for ~ 40% of modified cytosine brain and has been implicated methylation-related plasticity. To identify role 5hmC gene–environment interactions, we exposed both young (6-week-old) aged (18-month-old) mice enriched environment standard environment....
Alzheimer's disease (AD) is the most common form of neurodegenerative disorder that leads to a decline in cognitive function. In AD, aggregates amyloid β peptide precede accumulation neurofibrillary tangles, both which are hallmarks disease. The great majority (>90 %) AD cases not originated from genetic defects, therefore supporting central roles epigenetic modifications acquired progressively during life span. Strong evidences have indicated implication modifications, including histone...
Fragile X-associated tremor/ataxia syndrome (FXTAS) is a late-onset neurodegenerative disorder in which patients carry premutation alleles of 55–200 CGG repeats the FMR1 gene. To date, whether alterations epigenetic regulation modulate FXTAS has gone unexplored. 5-Hydroxymethylcytosine (5hmC) converted from 5-methylcytosine (5mC) by ten-eleven translocation (TET) family proteins been found recently to play key roles neuronal functions. Here, we undertook genome-wide profiling cerebellar 5hmC...
Curcuma longa (turmeric) is a folk medicine in South and Southeast Asia, which has been widely used to alleviate chronic inflammation. Aromatic-turmerone one of the main components abundant turmeric essential oil. However, little information available from controlled studies regarding its biological activities underlying molecular mechanisms against inflammation brain. In current study, we employed classical LPS model study effect mechanism aromatic-turmerone on neuroinflammation.The effects...
Autism spectrum disorders (ASDs) include a group of syndromes characterized by impaired language, social and communication skills, in addition to restrictive behaviors or stereotypes. However, with prevalence 1.5% developed countries high comorbidity rates, no clear underlying mechanism that unifies the heterogeneous phenotypes ASD exists. 5-hydroxymethylcytosine (5hmC) is highly enriched brain recognized as an essential epigenetic mark developmental disorders. To explore role 5hmC ASD, we...
Hematopoietic stem cells (HSC) maintain lifelong hematopoiesis. However, in current clonal analyses with unbiased “barcoding” approaches, steady-state hematopoietic clones young and middle-aged adults rarely have detectable HSCs, which precludes comprehensive interrogation of HSC behaviors. In the study, we used previously described Sleeping Beauty transposon model to investigate self-renewal differentiation at a level following chase that significantly enriched HSC-derived clones. From...
Hematopoietic stem cells (HSC) maintain lifelong hematopoiesis. However, in current clonal analyses with unbiased “barcoding” approaches, steady-state hematopoietic clones young and middle-aged adults rarely have detectable HSCs, which precludes comprehensive interrogation of HSC behaviors. In the study, we used previously described Sleeping Beauty transposon model to investigate self-renewal differentiation at a level following chase that significantly enriched HSC-derived clones. From...
Nephrotic syndrome is common in children and adults worldwide, steroid-sensitive nephrotic (SSNS) accounts for 80%. Aberrant metabolism involvement early SSNS sparsely studied, its pathogenesis remains unclear. Therefore, the goal of this study was to investigate changes initiated patients-related metabolites through serum urine metabolomics discover novel potential metabolic pathways.Serum samples (27 56 controls) (17 24 were collected. Meanwhile, non-targeted analyses performed by...
ABSTRACT Recently, combination therapy with acupuncture and medicine as a practical strategy to treat diseases has gained increasing attention. The present study aimed investigate whether stimulation at ST.36 had potential impact on the pharmacokinetics tissue distribution of lignans. An HPLC‐ESI/MS analytical method was established successfully applied comparative drug concentration in plasma tissues three parameters area under concentration–time curve from time zero final measurable point...
Abstract Normalization with respect to sequencing depth is a crucial step in single-cell RNA preprocessing. Most methods normalize data using the whole transcriptome based on assumption that majority of remains constant and are unable detect drastic changes transcriptome. Here, we develop an algorithm small fraction constantly expressed genes as internal spike-ins data. We demonstrate single cells may undergo several case study datasets accounting for such heterogeneity by ISnorm (Internal...
Angiotensin-I converting enzyme (ACE) has an important function in blood pressure regulation. ACEinhibitory peptides can lower by inhibiting ACE activity. In this investigation, water-soluble proteins were extracted from yeast (Saccharomyces cerevisiae) and hydrolyzed protein extraction to isolate peptides. Peptides with ACE-inhibitory activity further separated purified ultrafiltration fast liquid chromatography (FPLC). A hexapeptide, Thr-Pro-Thr-Gln-Gln-Ser, a calculated molecular weight...
Abstract Background Technical improvement in ATAC-seq makes it possible for high throughput profiling the chromatin states of single cells. However, data from multiple sources frequently show strong technical variations, which is referred to as batch effects. In order perform joint analysis across datasets, specialized method required remove variations between datasets while keep biological information. Results Here we present an algorithm named epiConv analyses on scATAC-seq datasets. We...
Cedrus deodara is a cedar plant from the Pinaceae family. Because widely used in health care products, cosmetics, medicine, and insecticides, essential oils of (CDEOs) are gaining increasing levels attention. However, there have only been few studies on anti-inflammatory effects CDEOs. This study aimed to systematically composition biological CDEOs, line with this, assessed chemical CDEOs evaluated their mechanisms mice. Gas chromatography-mass spectrometry (GC-MS) was determine analyze...
Hematopoietic stem cells (HSC) maintain lifelong hematopoiesis. However, in current clonal analyses with unbiased "barcoding" approaches, steady-state hematopoietic clones young and middle-aged adults rarely have detectable HSCs, which precludes comprehensive interrogation of HSC behaviors. In the study, we used previously described Sleeping Beauty transposon model to investigate self-renewal differentiation at a level following chase that significantly enriched HSC-derived clones. From...
Objective . The aim of this study was to evaluate the effectiveness traditional Chinese medicine- (TCM-) guided dietary interventions in improving yang-qi deficiency and yin-blood TCM syndromes according principles syndrome differentiation theory male youths undergoing drug detoxification during rehabilitation period who stayed a compulsory isolation center. Methods Male met criteria be included were randomly divided into intervention group ( n = 62) control 61) random number table 1 :...