A5060971312- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Microbial Community Ecology and Physiology
- Immunotherapy and Immune Responses
- Hearing, Cochlea, Tinnitus, Genetics
- Heavy Metal Exposure and Toxicity
- Bone Metabolism and Diseases
- Influenza Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Signaling Pathways in Disease
- Research in Cotton Cultivation
- Genetic Syndromes and Imprinting
- Ear Surgery and Otitis Media
- HIV Research and Treatment
- Effects and risks of endocrine disrupting chemicals
- Bioinformatics and Genomic Networks
- Aortic Disease and Treatment Approaches
- Aortic aneurysm repair treatments
- Neurological Disease Mechanisms and Treatments
- Hearing Loss and Rehabilitation
- Microbial Metabolism and Applications
Case Western Reserve University
2019-2025
Shandong Academy of Agricultural Sciences
2023-2025
University of Connecticut
2025
Shaanxi Normal University
2025
Wuhan University
2025
Hohai University
2023-2024
Jiaxing University
2017-2024
UConn Health
2021-2024
Second Xiangya Hospital of Central South University
2024
Central South University
2021-2024
DNA methylation is an important epigenetic modification that has essential roles in cellular processes including gene regulation, development and disease widely dysregulated most types of cancer. Recent advances sequencing technology have enabled the measurement at single nucleotide resolution through methods such as whole-genome bisulfite reduced representation sequencing. In studies, a key task to identify differences under distinct biological contexts, for example, between tumor normal...
DNA methylation is an important epigenetic modification involved in many biological processes and diseases. Recent developments whole genome bisulfite sequencing (WGBS) technology have enabled genome-wide measurements of at single base pair resolution. Many experiments been conducted to compare profiles under different contexts, with the goal identifying differentially methylated regions (DMRs). Due high cost WGBS experiments, studies are still without replicates. Methods tools available for...
Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs development function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed Asian ZIKVC, African ZIKVM, dengue (DENV). In contrast the robust global transcriptome changes induced by DENV, a more selective larger impact on expression genes involved in DNA replication repair. While overall profiles are similar, but not induces...
Cardiac failure is a major cause of death in patients with type 2 diabetes, but the molecular mechanism that links diabetes to heart remains unclear. Insulin resistance hallmark and insulin receptor substrates 1 (IRS1 IRS2) are insulin-signaling components regulating cellular metabolism survival. To determine role IRS1 IRS2 examine whether hyperinsulinemia causes myocardial dysfunction via IRS2, we generated heart-specific gene double-knockout (H-DKO) mice liver-specific (L-DKO) mice. H-DKO...
To evaluate the incidence and prevalence of cataract formation, progression, extraction in patients that underwent vitreoretinal procedures to factors can potentially predispose postoperative cataracts.The medical records consecutive who surgery at Yale Eye Center with least 6 months follow-up no prior intraocular were obtained. Preoperative, intraoperative, data recorded analyzed this retrospective observational study. The main outcome measures defined as extraction, progression after...
Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor (PAC1) play a critical role in biological processes that mediate stress response have been implicated psychological outcome following trauma. Our previous work [Ressler et al. (2011); Nature 470:492-497] demonstrated variant, rs2267735, the gene encoding PAC1 (ADCYAP1R1) is associated with post-traumatic disorder (PTSD) primarily African-American cohort of highly traumatized females. We sought to extend replicate our...
Drought stress seriously affects crop yield, and the mechanism underlying plant resistance to drought via macroautophagy/autophagy is not clear. Here, we show that a dehydrin, Medicago truncatula MtCAS31 (cold acclimation-specific 31), positive regulator of response, plays key role in autophagic degradation. A GFP cleavage assay treatment with an autophagy-specific inhibitor indicated participates degradation pathway overexpressing promotes autophagy under stress. Furthermore, discovered...
Abstract The pathogenesis of ulcerative colitis (UC), a major type inflammatory bowel disease, remains unknown. No model exists that adequately recapitulates the complexity clinical UC. Here, we take advantage induced pluripotent stem cells (iPSCs) to develop an human UC-derived organoid (iHUCO) and compared it with normal (iHNO). Notably, iHUCOs recapitulated histological functional features primary colitic tissues, including absence acidic mucus secretion aberrant adherens junctions in...
With the abundant emergence of remote sensing data sources, multimodal observation has become an active field. Extracting valuable information from multi-modal potential to make a significant contribution applications such as urban planning and monitoring. However, existing studies are deficient in extracting spectral spatial features hyperspectral data. Meanwhile, method fusing limitations poses challenge convergence model loss function, which increases complexity network optimisation...
Viroids are small, non-protein-coding RNAs which can induce disease symptoms in a variety of plant species. Potato (Solanum tuberosum L.) is the natural host spindle tuber viroid (PSTVd) where infection results stunting, distortion leaves and tubers yield loss. Replication PSTVd accompanied by accumulation viroid-derived small (sRNAs) proposed to play central role symptom development. Here we report that sRNAs direct RNA silencing potato against StTCP23, member TCP (teosinte...
Abstract Cells of the immune system are present in adult cochlea and respond to damage caused by noise exposure. However, types cells involved their locations within unclear. We used flow cytometry immunostaining reveal heterogeneity validated presence cell gene expression analyzing existing single-cell RNA-sequencing (scRNAseq) data. demonstrate that both innate adaptive cochlea. In response damage, increase number. B, T, NK, myeloid (macrophages neutrophils) predominant present....
Summary The HUB 2 gene encoding histone H2B monoubiquitination E3 ligase is involved in seed dormancy, flowering timing, defence response and salt stress regulation Arabidopsis thaliana . In this study, we used the cauliflower mosaic virus (Ca MV ) 35S promoter to drive At overexpression cotton found that it can significantly improve agricultural traits of transgenic plants under drought conditions, including increasing fruit branch number, boll boll‐setting rate decreasing abscission rate....
Silver nanoparticles (Ag NPs) displayed strong activities in anti-bacterial, anti-viral, and anti-fungal studies were reportedly efficient treating otitis media. Information on distribution of AgNPs different compartments the ear is lacking.To detect Ag NPs middle inner transportation pathways after transtympanic injection.Contrast effect micro CT imaging was assessed a phantom. at various concentrations (1.85 mM, 37.1 370.7 mM) administered to rat using injection cadaver heads imaged...
The proposition of cancer cells in a tumor sample, named as purity, is an intrinsic factor samples and has potentially great influence variety analyses including differential methylation, subclonal deconvolution subtype clustering. InfiniumPurify integrated R package for estimating accounting purity based on DNA methylation Infinium 450 k array data. three main functions getPurity, InfiniumDMC InfiniumClust, which could infer analysis sample cluster estimated or user-provided purities,...
Green manure (GM) is a crop commonly grown during fallow periods, which has been applied in agriculture as strategy to regulate nutrient cycling, improve organic matter, and enhance soil microbial biodiversity, but date, few studies have examined the effects of GM treatments on rhizosphere bacterial community metabolites from continuous cropping peanut field.In this study, we found that abundances several functionally significant groups containing Actinobacteria, Acidobacteria, genus...
A polycyclic aromatic hydrocarbons-degrading and acylhomoserine-lactone-producing marine bacterium, designated strain PQ-2 T , was isolated from biofilm collected a boat shell at harbour of Zhoushan island in Zhejiang Province, PR China. Strain is Gram-stain-negative, yellow-pigmented, non-motile short rod-shaped. Optimal growth observed 32 °C, pH 7.0 2 % (w/v) NaCl. The 16S rRNA gene sequence showed highest similarity to Croceicoccus marinus E4A9 (96.3 %) followed by Novosphingobium...
Bisulfite sequencing (BS‐seq) technology measures DNA methylation at single nucleotide resolution. A key task in BS‐seq data analysis is to identify differentially (DM) under different conditions. Here we provide a tutorial for DM using Bioconductor package DSS. DSS uses beta‐binomial model characterize the sequence counts from BS‐seq, and implements rigorous statistical method hypothesis testing. It provides flexible functionalities variety of analyses.
Loeys-Dietz syndrome (LDS) is an inherited disorder predisposing individuals to thoracic aortic aneurysm and dissection. Currently, there are no medical treatments except surgical resection. Although the genetic basis of LDS well-understood, molecular mechanisms underlying disease remain elusive, impeding development a therapeutic strategy. In addition, smooth muscle cells (SMCs) have heterogenous embryonic origins, depending on their spatial location, lineage-specific effects pathogenic...
Thoracic aortic aneurysm (TAA) is a life-threatening vascular disease frequently associated with underlying genetic causes. An inadequate understanding of human TAA pathogenesis highlights the need for better models. Here, we established functional model in an animal host by combining induced pluripotent stem cells (hiPSCs), bioengineered grafts (BVGs), and gene editing. We generated BVGs from isogenic control hiPSC-derived smooth muscle (SMCs) mutant SMCs gene-edited to carry Loeys-Dietz...