A5102864879- Chemical Synthesis and Analysis
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- RNA and protein synthesis mechanisms
- Cancer Mechanisms and Therapy
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- Genomics, phytochemicals, and oxidative stress
- Acute Lymphoblastic Leukemia research
- Bioactive Compounds and Antitumor Agents
- Traditional Chinese Medicine Analysis
- Ocular Oncology and Treatments
- Cancer therapeutics and mechanisms
- Antimicrobial Peptides and Activities
- Carcinogens and Genotoxicity Assessment
- Click Chemistry and Applications
- Computational Drug Discovery Methods
- Biopolymer Synthesis and Applications
- Cancer Treatment and Pharmacology
- MicroRNA in disease regulation
- Magnetic Field Sensors Techniques
Shanghai East Hospital
2025
Second Affiliated Hospital of Jilin University
2008-2024
China Medical University
2024
First Hospital of China Medical University
2024
Shanghai Tenth People's Hospital
2021-2022
Tongji University
2011-2022
Kunming Medical University
2021
Beijing University of Chinese Medicine
2017-2018
Army Medical University
2014-2015
Daping Hospital
2014-2015
Pancreatic ductal adenocarcinoma (PDA) is the fourth leading cause of cancer-related death in United States, with a 95% five-year mortality rate. For over decade, gemcitabine (GEM) has been established first-line treatment for this disease despite suboptimal response rates. The development PARP inhibitors that target DNA damage repair (DDR) system PDA cells generated encouraging results. Ubiquitin-specific peptidase 11 (USP11), an enzyme interacts DDR protein BRCA2, was recently discovered...
Polymorphism of DNA base excision repair (BER) genes affects capacity and may alter sensitivity to platinum‐based chemotherapy regimens. This study investigated polymorphisms OGG1 Ser326Cys, APE1 Asp148Glu ‐141T/G XRCC1 Arg399Gln for association with clinical outcome in 235 advanced inoperable nonsmall‐cell lung cancer (NSCLC) patients after treatment chemotherapy. The multivariate analysis showed that 326 GC was associated poor PFS [hazard ratio (HR) 1.730, p = 0.005], while 399 GA, or...
Resveratrol, a natural compound extracted from the skins of grapes, berries, or other fruits, has been shown to have anti-tumor effects against multiple myeloma (MM) via promoting apoptosis and inhibiting cell viability. In addition apoptosis, autophagy also plays significant role in effects. However, whether is involved anti-MM activity resveratrol remains unclear. this study, human MM lines U266, RPMI-8226, NCI-H929 were treated with resveratrol. Cell Counting Kit-8 assay colony formation...
Abstract Background Protein arginine methyltransferase 6 (PRMT6) plays a crucial role in various pathophysiological processes and diseases. Glioblastoma (GBM; WHO Grade 4 glioma) is the most common lethal primary brain tumor adults, with prognosis that extremely poor, despite being less than other systemic malignancies. Our current research finds PRMT6 upregulated GBM, enhancing malignancy. Yet, specifics of PRMT6’s regulatory potential molecular mechanisms GBM remain largely unexplored....
Abstract Background Enolase 1 (ENO1) is a conserved glycolytic enzyme that regulates glycolysis metabolism. However, its role beyond in the pathophysiology of multiple myeloma (MM) remains largely elusive. Herein, this study aimed to elucidate function ENO1 MM, particularly impact on mitophagy under bortezomib-induced apoptosis. Methods The bone marrow clinical MM patients and healthy normal donors was used compare expression level ENO1. Using online databases, we conducted an analysis...
Do you copy? A cell-permeable synthetic transcription factor mimic composed of a DNA-binding hairpin polyamide and an activation-domain-like peptoid can turn on gene in living cells. Supporting information for this article is available the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z604485_s.pdf or from author. Please note: The publisher not responsible content functionality any supporting supplied by authors. Any queries (other than missing content) should be directed to...
Spinocerebellar ataxia is a phenotypically and genetically heterogeneous group of autosomal dominant-inherited degenerative disorders. The gene mutation spectrum includes dynamic expansions, point mutations, duplications, insertions, deletions varying lengths. Dynamic expansion the most common form mutation. Mutations often result in indistinguishable clinical phenotypes, thus requiring validation using multiple genetic testing techniques. Depending on type mutation, pathogenesis may involve...
Transcriptional coactivator-binding peptoids were isolated from a large combinatorial library. One of these molecules is shown to function as potent activation domain surrogate in mammalian cells. Up 900-fold increase expression Gal4-responsive reporter gene observed when steroid conjugate the peptoid incubated with HeLa cells expressing Gal4 DNA-binding (DBD)−glucocorticoid receptor ligand-binding (GRLBD) fusion protein.
Synthetic molecules capable of activating the expression specific genes are great interest as tools for biological research and, potentially, a novel class pharmaceutical agents. It has been demonstrated previously that such synthetic transcription factor mimics (STFMs) can be constructed by connecting sequence-specific DNA-binding module to molecule binding transcriptional machinery via suitable linker. These chimeras mimic two basic properties native factors, which able recognize promoter...
Background: Numerous carcinogens and reactive oxygen species (ROS) may cause DNA damage including oxidative base lesions that lead to risk of nasopharyngeal carcinoma. Genetic susceptibility has been reported play a key role in the development this disease. The excision repair (BER) pathway can effectively remove lesions, maintaining genomic stability normal expression, with X-ray crosscomplementing1 (XRCC1), 8-oxoguanine glycosylase-1 (OGG1) apurinic/apyimidinic endonuclease 1 (APE1)...
Abstract: AT-101 is a BH3 mimetic and pan-Bcl-2 inhibitor that has shown potent anticancer activity in non-small-cell lung cancer (NSCLC) murine models, but failed to show clinical efficacy when used combination with docetaxel NSCLC patients. Our recent study demonstrated enhanced the antitumor effect of cisplatin (CDDP) model via inhibition interleukin-6/signal transducer activator transcription 3 (STAT3) pathway. This explored underlying mechanisms for CDDP by AT-101. results that,...
Pharmacologic agents capable of activating the expression specific genes would be valuable tools in biological research and could potentially useful therapeutically. Efforts to develop a general solution this problem have focused on discovery cell permeable mimics native transcription factors comprised linked DNA-binding activation domain surrogates. Recently, we reported isolation peptoid, called KBPo2, that binds fragment mammalian coactivator CREB-binding protein (CBP). When delivered...
Altered DNA repair capacity can result in increased susceptibility to cancer. The base excision (BER) pathway effectively removes damage caused by ionizing radiation and reactive oxidative species (ROS). In the current study, we analyzed possible relation of polymorphisms BER genes, including 8-oxoguanine glycosylase (OGG1), apurinic/apyrimidinic endonuclease 1 (APE1), X-ray cross-complementing group protein (XRCC1), with breast cancer risk Chinese Han women. This case-control study examined...
Growing evidence has shown that long non-coding RNAs (lncRNAs) play some roles in the progression of osteoarthritis. In this study, we investigated functions and mechanisms lncRNA NKILA (NKILA) chondrocytes human osteoarthritis (OA).RT-PCR was used to detect expressions miR-145 OA tissues. After transfection overexpression lentivirus (LV-NKILA) downregulation (LV-shNKILA) into primary chondrocytes, MTT assay carried out measure cell proliferation chondrocytes. The SP1, Bcl-2, Bax, cleaved...
To evaluate the anticancer effects of lupeol in retinoblastoma cells.WERI-Rb-1 and Y-79 cell lines were used to effect lupeol. After treatment, viability, proliferation, apoptosis, cancer stem-like properties, autophagy vivo tumour xenograft formation detected.In this study, decreased viability both WERI-Rb-1 lines. Lupeol could also inhibit proliferation induce apoptosis RB cells, with increased Bax level Ki67, survivin Bcl-2 levels. Furthermore, suppress spheroid properties cells....
Chromophore-assisted light inactivation (CALI) of proteins is a potentially powerful tool in biological research for the triggered disruption protein function. It involves creation chimeric molecules that can bind specifically to target and also sensitize photo-generation singlet oxygen, which inactivates protein. There remains need more efficient chromophores oxygen generation. Here we report general convenient system with evaluate efficiency CALI both crude extracts living cells. We employ...
In this study, the maximum tolerated dose (MTD) of lobaplatin (LBP) when it was combined with docetaxel (TXT) for treatment solid tumours that had progressed following chemotherapy determined, and toxicities to regimen were evaluated. A modified Fibonacci method used escalation LBP. The patients received TXT (at a fixed 60 mg/m2) on day one (d1) LBP an initial tested 30 two (d2) cycle repeated every 21 days. Each group consisted at least three cases. absence dose‑limiting toxicity (DLT), we...
Angelica sinensis root (ASR) extract was obtained to investigate its effects on colorectal carcinogenesis in different stages of an Azoxymethane/Dextran sodium sulphate (AOM/DSS) model. In this study, we showed that ASR administration the initial stage AOM/DSS model had cancer preventive with decreasing tumor incidence and a high-grade intraepithelial neoplasia incidence. With respect DNA damage, amounts 8-oxoguanine γ-H2AX were suppressed colon tissue. The balance apoptosis proliferation...
Abstract Background Aberrant DNA repair pathways contribute to malignant transformation or disease progression and the acquisition of drug resistance in multiple myeloma (MM); therefore, these could be therapeutically exploited. Ribonucleotide reductase (RNR) is rate-limiting enzyme for biosynthesis deoxyribonucleotides (dNTPs), which are essential replication damage repair. In this study, we explored efficacy novel RNR inhibitor, 4-hydroxysalicylanilide (HDS), cells xenograft model....
AIM: To explore the effect of parthenolide (PTL) on human uveal melanoma (UM) cells (C918 and SP6.5 cells) its molecular mechanism. METHODS: Carboxyfluorescein succinimidyl amino ester (CFSE) assays cell counting kit-8 (CCK-8) were performed to detect viability. Flow cytometry was used analyze cycle apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) Western blot measure proliferation-related apoptosis-related factors. RESULTS: Firstly, PTL decreased viability C918 in a...
Rearrangements of the mixed-lineage leukemia (MLL) gene occur predominately in pediatric cases and are generally predictors a poor prognosis. These chromosomal rearrangements result fusion protein MLL to one more than 60 partners. fusions potent inducers through activation oncogene expression; therefore, targeting this transcriptional function may arrest MLL-rearranged (MLL-R) leukemia. Leukemic cell lines harboring most common protein, MLL-AF4, require direct interaction AF4 with...