A5100362915- Extracellular vesicles in disease
- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- thermodynamics and calorimetric analyses
- Hedgehog Signaling Pathway Studies
- Field-Flow Fractionation Techniques
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Molecular Sensors and Ion Detection
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Multiple Myeloma Research and Treatments
- Acute Kidney Injury Research
- Chromatin Remodeling and Cancer
- Mass Spectrometry Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Dielectric materials and actuators
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
National Center for Nanoscience and Technology
2017-2024
University of Chinese Academy of Sciences
2018-2024
Chinese PLA General Hospital
2020-2024
Nanjing University
2023
Hubei University of Medicine
2022
Taihe Hospital
2022
First Affiliated Hospital of Xi'an Jiaotong University
2022
Sir Run Run Shaw Hospital
2022
Zhejiang University
2022
State Key Laboratory of Kidney Diseases
2020
Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30-200 nm) poses serious challenges in their isolation from complex media containing a variety extracellular vesicles (EVs) different sizes, especially sample volumes. Here we present viscoelasticity-based microfluidic system directly separate cell culture or serum continuous, size-dependent, and label-free...
Exosomal microRNAs (miRNAs) are reliable and noninvasive biomarkers for the early diagnosis of cancer. Yet, accurate feasible detection exosomal miRNAs is often hampered by low abundance in exosomes requirement RNA extraction large sample volumes. Here we show a thermophoretic sensor implemented with nanoflares situ miRNAs, without resorting to either or target amplification. Thermophoretic accumulation nanoflare-treated leads an amplified fluorescence signal upon binding nanoflares,...
Extracellular vesicles (EVs) are heavily implicated in diverse pathological processes. Due to their small size, distinct biogenesis, and heterogeneous marker expression, isolation detection of single EV subpopulations difficult. Here, we develop a λ-DNA- aptamer-mediated approach allowing for simultaneous size-selective separation surface protein analysis individual EVs. Using machine learning algorithm signature based on size demonstrate that the isolated microvesicles more efficient than...
Using natural membranes to coat nanoparticles (NPs) provides an efficient means reduce the immune clearance of NPs and improve their tumor-specific targeting. However, fabrication these drug-loaded biomimetic NPs, such as exosome membrane (EM)- or cancer cell (CCM)-coated poly(lactic-co-glycolic acid) (PLGA) remains a challenging task owing heterogeneous nature biomembranes labor-intensive procedures. Herein, we report microfluidic sonication approach produce EM-, CCM-, lipid-coated PLGA...
Abstract Molecular profiling of circulating extracellular vesicles (EVs) provides a promising noninvasive means to diagnose, monitor, and predict the course metastatic breast cancer (MBC). However, analysis EV protein markers has been confounded by presence soluble counterparts in peripheral blood. Here we use rapid, sensitive, low-cost thermophoretic aptasensor (TAS) profile cancer-associated profiles plasma EVs without interference proteins. We show that signature (a weighted sum eight...
Molecular profiling of tumor-derived extracellular vesicles (tEVs) holds great promise for non-invasive cancer diagnosis. However, sensitive and accurate identification tEVs is challenged by the heterogeneity EV phenotypes which reflect different cell origins. Here we present a DNA computation device mediated thermophoresis detection tEVs. The strategy leverages aptamer-based logic gate using multiple protein biomarkers on single EVs as input thermophoretic accumulation to amplify output...
Abstract Triple-negative breast cancer (TNBC) is a highly metastatic and heterogeneous type of with poor outcomes. Precise, non-invasive methods for diagnosis, monitoring prognosis TNBC are particularly challenging due to paucity biomarkers. Glycans on extracellular vesicles (EVs) hold the promise as valuable biomarkers, but conventional glycan analysis not feasible in clinical practice. Here, we report that lectin-based thermophoretic assay (EVLET) streamlines vibrating membrane filtration...
In response to environmental variations, living cells need arrange the conformational changes of macromolecules achieve specific biofunctions. Inspired by natural molecular machines, artificial macromolecular assemblies with controllable nanostructures and environmentally responsive functions can be designed. By assembling noble metal nanoparticles, information could significantly amplified modulated. However, manufacturing dynamic plasmonic that are efficiently different stimuli is still a...
Abstract The origin of homochirality in life is a fundamental mystery. Symmetry breaking and subsequent amplification chiral bias are regarded as one the underlying mechanisms. However, selection control initial spontaneous mirror symmetry process remains great challenge. Here we show experimental evidences that laminar microvortices generated within asymmetric microchambers can lead to hydrodynamic supramolecular systems composed exclusively achiral molecules milliseconds. self-assembled...
Abstract Circulating extracellular vesicles (EVs) have emerged as a valuable source of cancer biomarkers. However, the high degree EV heterogeneity and complexity clinical samples pose challenge in sensitive identification tumor‐derived EVs. Here we introduce one‐step thermophoretic AND gate operation (Tango) assay that integrates polyethylene glycol (PEG)‐enhanced accumulation EVs simultaneous on membranes by dual‐aptamers recognition. By using Tango to detect with co‐presence EpCAM PSMA...
Emerging evidence has revealed that gut microbiota plays a pivotal role in the pathogenesis of type 2 diabetes mellitus (T2DM) and diabetic kidney disease (DKD). However, few studies have used metagenomic sequencing to analyze alterations community patients with early-stage DKD.We carried out fecal samples 10 DKD (DKD group) T2DM who appeared be less prone (non-DKD group), aiming compare composition function between non-DKD groups.The microbial group was significantly different from group,...
ConspectusExtracellular vesicles (EVs) are cell-derived submicron bioparticles composed of lipid bilayer membrane and molecular cargos, acting as important mediators physiopathological cellular processes. The analysis engineering EVs hold significant therapeutic potential in noninvasive cancer diagnostics innovative drug delivery systems. Despite improvements technologies for EV investigation, the clinical use has been hampered by several challenges including requirement expensive equipment...
Tumor-derived extracellular vesicles (EVs) hold the potential to substantially improve noninvasive early diagnosis of cancer. However, analysis nanosized EVs in blood samples has been hampered by lack effective, rapid, and standardized methods for isolating detecting EVs. To address this difficulty, here we use electric-hydraulic analogy design cascaded microfluidic circuits pulsatile filtration via integration a cell-removal circuit an EV-isolation circuit. The device is solely driven...
The coating of natural cellular or exosomal membranes (EMs) onto polymeric nanoparticles has become essential in extending the circulation half-time by escaping from immune surveillance. Here we report on surface modification EM-coated poly(lactic-co-glycolic acid) (PLGA) AS1411 aptamers (AS-EP) for improved tumor targeting. combination microfluidic sonication and cholesterol-modified aptamer functionalization allows assembly AS-EP within 10 min. resulting shows a prolonged vivo time...
Abstract Chiral analysis of bioactive molecules is increasing significance in chemical and life sciences. However, the quantitative detection a racemic mixture enantiomers challenging task, which relies on complicated time‐consuming multiple steps chiral derivatization, separation, spectroscopic measurement. Herein, we show that, without use or pretreatment steps, co‐assembly amino acids with achiral TPPS 4 monomers controlled by enantiomorphic microvortices allows enantiomeric acids,...
The reconstruction of alveolar bone defect is essential for periodontal regenerative therapy or subsequent prosthetic/implant therapy, particularly in individuals with systemic diseases, such as osteoporosis. ROS accumulation, coupled aberrant macrophage polarization, pivotal the pathogenesis osteoporosis and contributes to disequilibrium between immune system. Consequently, therapeutic strategies targeting microenvironment present a promising approach treatment situ regeneration...
Purpose:Functional variants in the peroxisome proliferator‑activated receptor gamma (PPARG) and PPARGco-activator 1 (PPARGC1) family (e.g. PPARGC1A PPARGC1B) genes were predicted to confer susceptibility colorectal cancer (CRC).The aim of present study was explore relationship between PPARG polymorphism risk CRC. Patients methods:we conducted a case-control with 1,003 CRC cases 1,303 controls. We selected thePPARG rs3856806 C>T, rs2970847 rs8192678 rs3736265 G>A PPARGC1B rs7732671 G>C...
Background: NPM1 is highly expressed in a variety of solid tumors and promotes tumor development. However, there are few comprehensive studies on analysis gastrointestinal cancer. Methods: We used bioinformatics tools to study the expression difference between cancer control group, analyzed relationship its level diagnosis, prognosis, functional signaling pathway, immune infiltration, m6A cuproptosis related genes At same time, esophageal carcinoma (ESCA) samples was verified by vitro...
Abstract Enzymes encapsulated in extracellular vesicles (EVs) hold promise as biomarkers for early cancer diagnosis. However, precise measurement of their catalytic activities within EVs remains a notable challenge. Here, we report an enzymatically triggered spherical DNA nanomotor (EDM) that enables one‐pot, cascaded, and highly sensitive analysis the activity EV‐associated or free apurinic/apyrimidinic endonuclease 1 (APE1, key enzyme base excision repair) across various biological...