A5100390972- Extracellular vesicles in disease
- Immune cells in cancer
- MicroRNA in disease regulation
- Invertebrate Immune Response Mechanisms
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Aquaculture disease management and microbiota
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- Schizophrenia research and treatment
- Traditional Chinese Medicine Analysis
- Inflammation biomarkers and pathways
- Aquaculture Nutrition and Growth
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Macrophage Migration Inhibitory Factor
- RNA modifications and cancer
- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Galectins and Cancer Biology
West China Hospital of Sichuan University
2020-2025
Sichuan University
2020-2025
Central South University
2024-2025
Florida A&M University - Florida State University College of Engineering
2021-2025
Emory University
2012-2025
Jiangnan University
2025
Wuxi Fourth People's Hospital
2025
Nanjing University
2002-2024
Second Xiangya Hospital of Central South University
2024
National Clinical Research
2024
Abstract Molecular profiling of circulating extracellular vesicles (EVs) provides a promising noninvasive means to diagnose, monitor, and predict the course metastatic breast cancer (MBC). However, analysis EV protein markers has been confounded by presence soluble counterparts in peripheral blood. Here we use rapid, sensitive, low-cost thermophoretic aptasensor (TAS) profile cancer-associated profiles plasma EVs without interference proteins. We show that signature (a weighted sum eight...
Human mesenchymal stem cell (hMSC) derived extracellular vesicles (EVs) have shown therapeutic potential in recent studies. However, the corresponding components are largely unknown, and scale-up production of hMSC EVs is a major challenge for translational applications. In current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion. Results demonstrate that activation endosomal sorting complexes required transport (ESCRT)-dependent -independent pathways. mRNA...
Knee osteoarthritis (KOA) is one of the most common chronic musculoskeletal disorders worldwide, for which exosomes derived from stem cells may provide an effective treatment.To assess effect human urine-derived (hUSCs) overexpressing miR-140-5p (miR means microRNA) on KOA in vitro interleukin 1β (IL-1β)-induced (OA) model and vivo rat model.Controlled laboratory study.Exosomes hUSCs (hUSC-Exos) were isolated validated. The transfected with miR-140s using lentivirus, secreted such...
Patients with hematogenous metastatic lung cancer displayed significantly increased platelet count and aggregation compared to patients without metastasis. The mechanism underlying the correlation between metastasis activation remains unknown. In present study, we explored role of microRNA-223 (miR-223) derived from platelets in modulating cell invasion. Our results demonstrated that NSCLC contain higher level miR-223 than healthy subjects. concentration platelet-secreted microvesicles...
Arterial calcification is a key pathologic component of vascular diseases such as atherosclerosis, coronary artery disease, and peripheral disease. A hallmark this pathological process the phenotypic transition smooth muscle cells (VSMCs) to osteoblast-like cells. Several studies have demonstrated that microRNAs (miRNAs) regulate osteoblast differentiation, but it unclear whether miRNAs also VSMC-mediated arterial calcification. In present study, we sought characterize role miR-133a in...
Abstract Recently, therapies utilizing extracellular vesicles (EVs) derived from mesenchymal stromal/stem cells (MSCs) have begun to show promise in clinical trials. However, EV therapeutic potential varies with MSC tissue source and vitro expansion through passaging. To find the optimal for clinically translatable EV‐derived therapies, this study aims compare angiogenic immunomodulatory potentials protein miRNA cargo compositions of EVs isolated two most common sources adult MSCs, bone...
Abstract Small RNAs (microRNAs [miRNAs] or small interfering [siRNAs]) are effective tools for cancer therapy, but many of the existing carriers their delivery limited by low bioavailability, insufficient loading, impaired transport across biological barriers, and into tumor microenvironment. Extracellular vesicle (EV)–based communication in mammalian plant systems is important physiological pathological processes, EVs show promise as RNA interference molecules. However, some fundamental...
We aim to explore whether the effect of oral celecoxib (OC) preoperatively in elderly patients with femoral neck fracture (FNF) receiving surgery on pain control, complications, functional outcomes, and mortality. collected data FNF two hospitals between Jan. 2020 May. 2024. According OC use or not, were divided into group non-OC group. performed propensity score matching (PSM) analysis minimize potential confounding selection bias. observed factors that may influence control by Spearman...
G-quadruplex/hemin (G4/hemin) DNAzymes are biosensing systems, but their application remains limited by an overall low activity and a rather high level of unwarranted background reactions. Here, these issues were addressed through the rational design F3T-azaC-hemin, G4-based construct in which hemin is covalently linked to G4 core its binding site flanked with nucleotide activator, here d(T-azaC). This led G4-DNAzyme whose performances have been ca. 150-fold increased compared parent system....
Chimeric peptide-DNAzyme (CPDzyme) is a novel artificial peroxidase that relies on the covalent assembly of DNA, peptides, and an enzyme cofactor in single scaffold. An accurate control these different partners allows for design CPDzyme prototype G4-Hemin-KHRRH, found to be >2000-fold more active (in terms conversion number kcat) than corresponding but non-covalent G4/Hemin complex and, importantly, >1.5-fold native (horseradish peroxidase) when considering catalytic center. This unique...
Abstract Background Following spinal cord injury (SCI), the inflammatory storm initiated by microglia/macrophages poses a significant impediment to recovery process. Exosomes play crucial role in transport of miRNAs, facilitating essential cellular communication through transfer genetic material. However, miRNAs from iPSC-NSCs-Exos and their potential mechanisms leading repair after SCI remain unclear. This study aims explore microglia/macrophage pyroptosis reveal mechanisms. Methods were...
Tumor‐associated macrophages ( TAM ) play a critical role in promoting tumor development and metastasis. In the present study, we found that legumain, an asparaginyl endopeptidase, was highly expressed on surface of . A doxorubicin‐based prodrug specifically activated by legumain selectively ablated resulted significant reduction angiogenic factors related vessel growth. Treatment with also suppressed circulating cells myeloid immune suppressor Gr‐1+/CD11b+ tumor‐bearing animals. After...
The protein tyrosine phosphatase PTP 1B, which is encoded by PTPN 1, a ubiquitously expressed nonreceptor phosphatase. 1B has long been known to negatively regulate insulin and leptin receptor signalling. Recently, it was reported be aberrantly in cancer cells function as an important oncogene. In this study, we found that levels are dramatically increased breast (BC) tissues promotes the proliferation, suppresses apoptosis, of both HER 2‐positive triple‐negative BC cell lines....
Abstract Chronic diseases are often associated with altered inflammatory response, leading to increased host vulnerability new challenges. Employing streptozotocin (STZ)-induced diabetes as a model, we further investigate mechanisms enhanced neutrophil (polymorphonuclear leukocytes [PMN]) responses under hyperglycemia and compare them those chronic colitis. We show that, different from colitis which the PMN response is significantly potentiated, existence of proinflammatory state broad...
Extracellular vesicles (EVs) are enclosed by a nanoscale phospholipid bilayer membrane and typically range in size from 30 to 200 nm.They contain high concentration of specific proteins, nucleic acids, lipids, reflecting but not identical the composition parent cell.The inherent characteristics variety EVs give them extensive unique advantages field cancer identification treatment.Recently, have been recognized as potential tumor markers for detection cancer.Aptamers, which molecules...
Emerging evidence indicates that IGF2 plays an important role in various human malignancies, including colorectal cancer (CRC). Hsa-miR-483 is located within intron 7 of the locus. However, mechanism by which increased induces carcinogenesis remains largely elusive. DLC-1 has been identified as a candidate tumor suppressor. In this study, we aimed at investigating whether miR-483 transcription IGF2-dependent, identifying functional target miR-483, and evaluating tissue serum miR-483-3p or...