Michael H. A. Roehrl
A5002327806- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Research Studies
- Colorectal Cancer Treatments and Studies
- Epigenetics and DNA Methylation
- Neuroendocrine Tumor Research Advances
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Colorectal and Anal Carcinomas
- Immunotherapy and Immune Responses
- Lung Cancer Treatments and Mutations
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- Systemic Lupus Erythematosus Research
- Diabetes and associated disorders
- Ferroptosis and cancer prognosis
- Renal cell carcinoma treatment
- Signaling Pathways in Disease
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Evolution and Genetic Dynamics
Harvard University
2003-2025
Beth Israel Deaconess Medical Center
2023-2025
Memorial Sloan Kettering Cancer Center
2016-2024
University Health Network
2014-2024
University of Toronto
2014-2024
Hadassah Medical Center
2024
Okayama University
2024
Kawasaki Medical School
2024
Princess Margaret Cancer Centre
2015-2024
Lemuel Shattuck Hospital
2024
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer with poor patient survival. Toward understanding the underlying molecular alterations that drive PDAC oncogenesis, we conducted comprehensive proteogenomic analysis of 140 pancreatic cancers, 67 normal adjacent tissues, and 9 tissues. Proteomic, phosphoproteomic, glycoproteomic analyses were used to characterize proteins their modifications. In addition, whole-genome sequencing, whole-exome methylation, RNA sequencing...
Avian Flu in Ferrets A recent outbreak of avian H7N9 influenza humans eastern China has been closely monitored for any evidence human-to-human transmission and its potential sparking a pandemic. Zhu et al. (p. 183 , published online 23 May) examined the behavior virus ferret, mammalian model human influenza. The was excreted by ferrets could be transmitted readily contact but displayed limited capacity airborne infectivity. pathology is similar to H1N1, it seems that factors other than...
The clinical utility of molecular profiling tumor tissue to guide treatment patients with advanced solid tumors is unknown. Our objectives were evaluate the frequency genomic alterations, "actionability" somatic variants, enrollment in mutation-targeted or other trials, and outcome for at Princess Margaret Cancer Centre (PM). Patients aged ≥18 years, good performance status, archival available prospectively consented. DNA from formalin-fixed paraffin-embedded was tested using a MALDI-TOF MS...
<h3>Importance</h3> Outcomes for patients with pancreatic ductal adenocarcinoma (PDAC) remain poor. Advances in next-generation sequencing provide a route to therapeutic approaches, and integrating DNA RNA analysis clinicopathologic data may be crucial step toward personalized treatment strategies this disease. <h3>Objective</h3> To classify PDAC according distinct mutational processes, explore their clinical significance. <h3>Design, Setting, Participants</h3> We performed retrospective...
The purpose of this study was to evaluate the prognostic value Immunoscore in patients with stage III colon cancer (CC) and analyze its association effect chemotherapy on time recurrence (TTR).
Equilibrium binding experiments are widely used for the accurate characterization of and competitive behavior in biological systems. Modern high-throughput discovery efforts chemical biology rely heavily upon this principle. Here, we derive exact analytical expressions general models which can also explain a commonly encountered phenomenon these types experiments, anticooperative incomplete displacement. We explore effects nonspecific parameter misestimation. All derived terms total...
The combinatorial action of separate cis-acting elements controls the cell-specific expression type I collagen genes. In particular, we have shown that two short located between -3.2 and -2.3 kb named TSE1 TSE2 are needed for mouse COL1a1 gene in tendon fibroblasts. this study, analyzed trans-acting factors binding to TSE2. Gel shift experiments showed scleraxis (SCX), which is a basic helix-loop-helix transcription factor expressed selectively fibroblasts, binds TSE2, preferentially as...
Lineage plasticity is implicated in treatment resistance multiple cancers. In lung adenocarcinomas (LUAD) amenable to targeted therapy, transformation small cell cancer (SCLC) a recognized mechanism. Defining molecular mechanisms of neuroendocrine (NE) has been limited by paucity pre/posttransformation clinical samples. Detailed genomic, epigenomic, transcriptomic, and protein characterization combined LUAD/SCLC tumors, as well samples, supports that NE primarily driven transcriptional...
Broad adoption of digital pathology (DP) is still lacking, and examples for DP connecting diagnostic, research, educational use cases are missing. We blueprint a holistic solution at large academic medical center ubiquitously integrated into clinical workflows; researchapplications including molecular, genetic, tissue databases; processes.
Abstract The utility of cancer whole genome and transcriptome sequencing (cWGTS) in oncology is increasingly recognized. However, implementation cWGTS challenged by the need to deliver results within clinically relevant timeframes, concerns about assay sensitivity, reporting prioritization findings. In a prospective research study we develop workflow that reports comprehensive 9 days. Comparison diagnostic panel assays demonstrates potential capture all reported mutations with comparable...
Since 2010, the Human Proteome Project (HPP), flagship initiative of Organization (HUPO), has pursued two goals: (1) to credibly identify protein parts list and (2) make proteomics an integral part multiomics studies human health disease. The HPP relies on international collaboration, data sharing, standardized reanalysis MS sets by PeptideAtlas MassIVE-KB using Guidelines for quality assurance, integration curation non-MS neXtProt, plus extensive use antibody profiling carried out Protein...
Transient or reversible protein–protein interactions are commonly used to ensure efficient targeting of signaling enzymes their cellular substrates. These include direct binding substrate, interaction with an accessory scaffold protein, and positioning at subcellular locations in proximity The existence specialized mechanisms raises the possibility designing inhibitors that do not block enzyme activity per se , but rather interfere one more its substrates within cell. Here, we identify small...
Discovery of tissue-specific biomarkers for human cancer is crucial early diagnosis and molecular understanding the disease. To overcome limitations posed by large dynamic concentration range compositional complexity tissue biomacromolecules, we applied heparin affinity fractionation proteomic enrichment. Comparing proteomes five paired samples normal lung pulmonary adenocarcinoma 2-D difference gel electrophoresis, 14 spots were found to be differentially expressed. From these candidate...
3500 Background: Mounting evidence indicates an enhanced lymphocytic reaction as informative prognostic indicator in colon cancer (CC). The IM methodology has been defined to quantify the situ immune infiltrate. Methods: A Society for Immunotherapy of Cancer-led international consortium 23 pathology expert centers from 17 countries was initiated evaluate standardized assay routine clinical settings. Patient (pt) eligibility criteria were CC Stages I/II/III and no neo-adjuvant treatment. 1336...
Immunotherapy with monoclonal antibodies targeting cytotoxic T-lymphocyte antigen 4 (CTLA-4) or programmed cell death 1 (PD-1) has improved the survival of patients metastatic melanoma. These agents carry a certain risk adverse immune-related events. We present patient widely melanoma who was initially treated ipilimumab and subsequently nivolumab. After four infusions nivolumab, he developed subacute multifocal central nervous system (CNS) demyelination. Nivolumab discontinued and, despite...