A5062444950- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- Head and Neck Cancer Studies
- Immune Cell Function and Interaction
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Treatments and Mutations
- Chemokine receptors and signaling
- Colorectal and Anal Carcinomas
- Lymphoma Diagnosis and Treatment
- AI in cancer detection
- Immune cells in cancer
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Biomedical Text Mining and Ontologies
- Nanoplatforms for cancer theranostics
Providence Portland Medical Center
2016-2025
Providence College
2013-2024
Providence Health & Services
2014-2024
ID Genomics (United States)
2024
Society for Immunotherapy of Cancer
2015-2024
Cancer Research Center
2013-2023
St. Joseph Health System
2020-2022
Cancer Institute (WIA)
2014-2021
American Association For Cancer Research
2021
University of Lisbon
2014-2018
Abstract Enzymatic and non‐enzymatic treatments for antigen unmasking on formalin‐fixed, paraffin‐embedded, dewaxed sections were optimized compared by the use of a panel antibodies diagnostic relevance (anti‐cytokeratins, vimentin. S‐100. T‐ B‐cell receptors, Ki‐67/MIB 1, muscle actin). Non‐enzymatic was obtained boiling slides in microwave oven 0.01 M salt solution (pH 6) or 6 urea. Trypsin pronase digestion used comparison found to be necessary some reagents. The investigation then...
Head and neck cancers, including those of the lip oral cavity, nasal paranasal sinuses, oropharynx, larynx nasopharynx represent nearly 700,000 new cases 380,000 deaths worldwide per annum, account for over 10,000 annual in United States alone. Improvement outcomes are needed patients with recurrent or metastatic squamous cell carcinoma head (HNSCC). In 2016, US Food Drug Administration (FDA) granted first immunotherapeutic approvals - anti-PD-1 immune checkpoint inhibitors nivolumab...
Abstract. Drought is a natural hazard that can cause wide range of impacts affecting the environment, society, and economy. Providing an impact assessment reducing vulnerability to these for regions beyond local scale, spanning political sectoral boundaries, requires systematic detailed data regarding impacts. This study presents diversity drought across Europe based on European Impact report Inventory (EDII), unique research database has collected close 5000 reports from 33 countries. The...
Objectives The interaction between the immune system and tumor cells is an important feature for prognosis treatment of cancer. Multiplex immunohistochemistry (mIHC) multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify cell subsets, their functional state, spatial arrangement within microenvironment. Methods Society Immunotherapy Cancer (SITC) convened a task force pathologists laboratory leaders from academic centers as well experts...
The purpose of this study was to evaluate the prognostic value Immunoscore in patients with stage III colon cancer (CC) and analyze its association effect chemotherapy on time recurrence (TTR).
Background Checkpoint inhibitors targeting programmed death receptor-1 (PD-1) have been tested in the neoadjuvant setting for treatment of locoregionally advanced head and neck squamous cell carcinoma (HNSCC); however, response rates are modest. We hypothesized that adding stereotactic body radiation therapy (SBRT) to anti-PD-1 would be safe prior definitive surgical resection enhance pathological compared with historical cohorts patients HNSCC treated checkpoint inhibitor alone. Methods The...
Pancreatic ductal adenocarcinoma (PDAC) has traditionally been thought of as an immunologically quiescent tumor type presumably because a relatively low mutational burden (TMB) and poor responses to checkpoint blockade therapy. However, many PDAC tumors exhibit T cell inflamed phenotypes. The presence tertiary lymphoid structures (TLS) recently shown be predictive response in melanomas sarcomas, are prognostic for survival PDAC. In order more comprehensively understand immunity patients with...
Despite the success of checkpoint blockade in some cancer patients, there is an unmet need to improve outcomes. Targeting alternative pathways, such as costimulatory molecules (e.g. OX40, GITR, and 4-1BB), can enhance T cell immunity tumor-bearing hosts. Here we describe results from a phase Ib clinical trial (NCT02274155) which 17 patients with locally advanced head neck squamous carcinoma (HNSCC) received murine anti-human OX40 agonist antibody (MEDI6469) prior definitive surgical...
Abstract Digital pathology poses unique computational challenges, as a standard gigapixel slide may comprise tens of thousands image tiles 1–3 . Prior models have often resorted to subsampling small portion for each slide, thus missing the important slide-level context 4 Here we present Prov-GigaPath, whole-slide foundation model pretrained on 1.3 billion 256 × in 171,189 whole slides from Providence, large US health network comprising 28 cancer centres. The originated more than 30,000...
Evaluation of T lymphocyte frequency provides prognostic information for patients with oral squamous cell cancer (OSCC). However, the effect simultaneously evaluating and assessing suppressive elements defects in antigen-processing machinery (APM) has not been clarified. Simultaneous characterization CD3+, CD8+, FoxP3+, CD163+, PD-L1+ cells using multispectral imaging was performed on sections from 119 HPV- OSCC. Expression β2-microglobulin, MHC class I heavy chain, large multifunctional...
Basal cell carcinoma (BCC) and cutaneous squamous (CSCC) share exposure to UV light as the dominant risk factor, these tumors therefore harbor high mutation burdens. In other malignancies, burden has been associated with clinical benefit from therapy antibodies directed against Programmed Death 1 (PD-1) immune checkpoint receptor. Highly mutated are more likely express immunogenic tumor neoantigens that attract effector T cells, which can be unleashed by blockade of PD-1 checkpoint.This...
The fifth "Melanoma Bridge Meeting" took place in Naples, December 1–5th, 2015. main topics discussed at this meeting were: Molecular and Immuno advances, Immunotherapies Combination Therapies, Tumor Microenvironment Biomarkers Immunoscore. natural history of cancer involves interactions between the tumor immune system host. infiltration site may be indicative host response. Significant correlations were shown levels cell tumors patient's clinical outcome. Moreover, incredible progress comes...
Adoptive T cell therapy (ACT) has shown great promise in melanoma, with over 50 % response rate patients where autologous tumor-reactive tumor-infiltrating lymphocytes (TIL) can be cultured and expanded. A major limitation of ACT is the inability to generate or expand TIL 25-45 tested. Methods that successfully identify tumors are not suitable for generation by standard methods would eliminate costs fruitless expansion enable these receive alternate immediately.Multispectral fluorescent...
Interleukin 12 (IL-12) is a pivotal regulator of innate and adaptive immunity. We conducted prospective open-label, phase II clinical trial electroporated plasmid IL-12 in advanced melanoma patients (NCT01502293).Patients with stage III/IV were treated intratumorally encoding (tavokinogene telseplasmid; tavo), 0.5 mg/ml followed by electroporation (six pulses, 1500 V/cm) on days 1, 5, 8 every 90 the main study additional two alternative schedule exploration cohorts. Correlative analyses for...
(1) Background: Digital pathology (DP) is transforming the landscape of clinical practice, offering a revolutionary approach to traditional analysis and diagnosis. (2) Methods: This innovative technology involves digitization glass slides which enables pathologists access, analyze, share high-resolution whole-slide images (WSI) tissue specimens in digital format. By integrating cutting-edge imaging with advanced software, DP promises enhance practice numerous ways. not only improves quality...
BackgroundBiomedical data are inherently multimodal, comprising physical measurements and natural-language narratives. A generalist biomedical artificial intelligence (AI) model needs to simultaneously process different modalities of data, including text images. Therefore, training an effective requires high-quality multimodal such as parallel image–text pairs.MethodsHere, we present PMC-15M, a novel dataset that is two orders magnitude larger than existing datasets, MIMIC-CXR, spans diverse...
Objectives Multiplex immunohistochemistry and immunofluorescence (mIHC/IF) are emerging technologies that can be used to help define complex immunophenotypes in tissue, quantify immune cell subsets, assess the spatial arrangement of marker expression. mIHC/IF assays require concerted efforts optimize validate multiplex staining protocols prior their application on slides. The best practice guidelines for validation across platforms were previously published by this task force. current effort...
Immunotherapy of cancer is now an essential pillar treatment for patients with many individual tumor types. Novel immune targets and technical advances are driving a rapid exploration new strategies incorporating agents in clinical practice. Immunotherapies perturb complex system interactions among genomically unstable cells, diverse cells within the microenvironment including systemic adaptive innate cells. The drive to develop increasingly effective immunotherapy regimens tempered by risk...