Michal Vočka
A5076719276- BRCA gene mutations in cancer
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- Ovarian cancer diagnosis and treatment
- Nutrition, Genetics, and Disease
- Cancer Treatment and Pharmacology
- Lung Cancer Treatments and Mutations
- DNA Repair Mechanisms
- Colorectal Cancer Surgical Treatments
- Multiple and Secondary Primary Cancers
- Cancer Cells and Metastasis
- Intraperitoneal and Appendiceal Malignancies
- Colorectal and Anal Carcinomas
- Sarcoma Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Genomics and Rare Diseases
- PARP inhibition in cancer therapy
- Immune Cell Function and Interaction
- Hepatocellular Carcinoma Treatment and Prognosis
- Genomic variations and chromosomal abnormalities
- Metastasis and carcinoma case studies
Charles University
2015-2024
General University Hospital in Prague
2015-2024
Ocní klinika
2015-2021
Onkologikoa
2021
Czech Academy of Sciences, Institute of Molecular Genetics
2019
Centrum kardiovaskulární a transplantační chirurgie
2019
University Hospital Plzen
2017
Military University Hospital Prague
2015
The purpose of this study was to evaluate the prognostic value Immunoscore in patients with stage III colon cancer (CC) and analyze its association effect chemotherapy on time recurrence (TTR).
Pancreatic ductal adenocarcinoma (PDAC), the most deadly solid malignancy, is typically detected late and at an inoperable stage. Early or incidental detection associated with prolonged survival, but screening asymptomatic individuals for PDAC using a single test remains unfeasible due to low prevalence potential harms of false positives. Non-contrast computed tomography (CT), routinely performed clinical indications, offers large-scale screening, however, identification non-contrast CT has...
Germline mutations in checkpoint kinase 2 ( CHEK2 ), a multiple cancer‐predisposing gene, increase breast cancer (BC) risk; however, risk estimates differ substantially published studies. We analyzed germline variants 1,928 high‐risk Czech breast/ovarian (BC/OC) patients and 3,360 population‐matched controls (PMCs). For functional classification of VUS, we developed complementation assay human nontransformed RPE1‐ ‐knockout cells quantifying CHK2‐specific phosphorylation endogenous protein...
Background: The prognostic value of Immunoscore was evaluated in Stage II/III colon cancer (CC) patients, but it remains unclear I/II, and early-stage subgroups at risk. An international Society for Immunotherapy Cancer (SITC) study the pre-defined consensus tumors from 1885 AJCC/UICC-TNM I/II CC patients Canada/USA (Cohort 1) Europe/Asia 2). METHODS: Digital-pathology is used to quantify densities CD3+ CD8+ T-lymphocyte center tumor (CT) invasive margin (IM). time recurrence (TTR) primary...
Abstract RAD18 is an E3 ubiquitin ligase that prevents replication fork collapse by promoting DNA translesion synthesis and template switching. Besides this classical role, has been implicated in homologous recombination; however, function incompletely understood. Here, we show recruited to lesions monoubiquitination of histone H2A at K15 counteracts accumulation 53BP1. Super-resolution microscopy revealed localizes the proximity double strand breaks limits distribution 53BP1 peripheral...
Ovarian cancer (OC) is the deadliest gynecologic malignancy with a substantial proportion of hereditary cases and frequent association breast (BC). Genetic testing facilitates treatment preventive strategies reducing OC mortality in mutation carriers. However, prevalence germline mutations varies among populations many rarely mutated predisposition genes remain to be identified. We aimed analyze 219 1333 Czech patients 2278 population-matched controls using next-generation sequencing....
Tissue inhibitor of metalloproteinases 1 (TIMP-1) and matrix metalloproteinase 7 (MMP-7) were reported to have potent growth promoting activity. Lack balance between MMPs TIMPs is an important factor in the development gastrointestinal malignancies.We collected serum samples from 97 patients with metastatic colorectal cancer 79 healthy controls. Serum levels TIMP-1 MMP-7 measured immunochemically compared standard tumor markers carcinoembryonic antigen CA19-9.Serum significantly higher...
Germline CHEK2 pathogenic variants confer an increased risk of female breast cancer (FBC). Here we describe a recurrent germline intronic variant c.1009-118_1009-87delinsC, which showed splice acceptor shift in RNA analysis, introducing premature stop codon (p.Tyr337PhefsTer37). The was found 21/10,204 (0.21%) Czech FBC patients compared to 1/3250 (0.03%) controls (p = 0.04) and 4/3639 (0.11%) from independent German dataset. In addition, this 5/2966 (0.17%) (but none the 443 German) ovarian...
Abstract The subset of ovarian cancer (OC) diagnosed ≤ 30yo represents a distinct subgroup exhibiting disparities from late-onset OC in many aspects, including indefinite germline predisposition. We performed DNA/RNA-WES with HLA-typing, PRS assessment and survival analysis 123 early-onset OC-patients compared to histology/stage-matched unselected OC-patients, population-matched controls. Only 6/123(4.9%) carried pathogenic variant (GPV) high-penetrance OC-predisposition genes. Nevertheless,...
Hereditary breast cancer comprises a minor but clinically meaningful ( BC ) subgroup. Mutations in the major ‐susceptibility genes are important prognostic and predictive markers; however, their carriers represent only 25% of high‐risk patients. To further characterize variants influencing risk, we performed SOLiD sequencing 581 325 patients (negatively tested previous BRCA1 / BRCA2 PALB2 analyses). In 105 (32%) patients, identified confirmed 127 truncating (89 unique; nonsense, frameshift...
BACKGROUND: GDF-15 is a protein belonging to the transforming growth factor beta superfamily that has role in regulating inflammatory and apoptotic pathways. High level tumor tissues plasma correlate with an increased risk of recurrence reduced overall survival. OBJECTIVE: The im this study was screen capacity detecting metastatic CRC compare it standard markers CEA CA19-9. METHODS: We collected serum samples from 97 patients colorectal cancer 79 healthy controls. Serum levels GDF-15, CA19-9...
Background Carriers of mutations in hereditary cancer predisposition genes represent a small but clinically important subgroup oncology patients. The identification causal germline determines follow-up management, treatment options and genetic counselling patients' families. Targeted next-generation sequencing-based analyses using cancer-specific panels high-risk individuals have been rapidly adopted by diagnostic laboratories. While the use diagnosis-specific is straightforward typical...
Colorectal cancer (CRC) is the second leading tumor diagnosis in women and men Czech Republic. Patient outcome depends on stage at time of and, metastatic disease, localization extent distant metastases. The early detection liver disease an important indication for surgery. Therefore, novel biomarkers are urgently required. Serum samples were collected from 97 patients with histologically confirmed CRC or progression during palliative treatment, 79 healthy controls. All exhibited adequate...
Breast cancer (BC) prognosis in BRCA1 and BRCA2 mutation carriers has been reported contradictorily, the significance of variables influencing sporadic BC is not established patients with hereditary BRCA1/BRCA2 mutations. In this retrospective cohort study, we analyzed effect clinicopathological characteristics on (disease-free survival [DFS] disease-specific [DSS]) carriers. We enrolled 234 899 non-carriers, whom 191 680 complete data, were available for analyses. found that ER-positive...
Abstract Purpose Immune checkpoint inhibitors (ICIs) dramatically changed the prognosis of patients with NSCLC. Unfortunately, a reliable predictive biomarker is still missing. Commonly used biomarkers, such as PD-L1, MSI, or TMB, are not quite accurate in predicting ICI efficacy. Methods In this prospective observational cohort study, we investigated role erythrocytes, thrombocytes, innate and adaptive immune cells, complement proteins (C3, C4), cytokines from peripheral blood 224 stage...
Endometrial cancer (EC) is the most common gynecological malignancy in developed countries. The present study aimed to determine frequency of germline pathogenic variants (PV) patients with EC. In this multicenter retrospective cohort study, genetic testing (GGT) was performed 527 EC using a next generation sequencing panel targeting 226 genes, including 5 Lynch syndrome (LS) and 14 hereditary breast ovarian (HBOC) predisposition 207 candidate genes. Gene-level risks were calculated 1,662...
The Guidelines for Clinical Practice carriers of pathogenic variants in clinically relevant cancer predisposition genes define the steps primary and secondary prevention that should be provided to these individuals at high risk developing hereditary Czech Republic. drafting guidelines was organized by Oncogenetics Working Group Society Medical Genetics Genomics J. E. Purkyně (SLG ČLS JEP) cooperation with representatives oncology oncogynecology. are based on current recommendations National...
Background: The objective of this study was to investigate serum levels immunosuppressive cytokines TGF beta 1 and VEGF count immune cells in peripheral blood stage II III colorectal cancer patients.Methods: Blood samples were collected from 22 patients 25 healthy controls before the start treatment. All examined by a clinical immunologist exclude with disorders autoimmune diseases. measured ELISA, anti-tumor cellular immunity (CD4, CD8, B cells, NK cells) flow cytometry.Results: plasma...
Trefoil factor family (TFF) is composed of three secretory proteins (TFF1, TFF2 and TFF3) that play an important role in mucosal protection gastrointestinal tract. Their overexpression colorectal tumors seems to be associated with more aggressive disease. We collected serum samples from 79 healthy controls 97 patients metastatic cancer at the time diagnosis or progression. Serum levels TTF1-3, CEA CA19-9 were measured by ELISA. TFF1 TFF3 significantly higher compared (p < 0.0001). Moreover,...
The distribution of ovarian tumour characteristics differs between germline BRCA1 and BRCA2 pathogenic variant carriers non-carriers. In this study, we assessed the utility as predictors pathogenicity, for application using American College Medical Genetics Association Molecular Pathology (ACMG/AMP) classification system.