Amos Kirilovsky
A5053292654- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Surgical Treatments
- Colorectal Cancer Treatments and Studies
- Colorectal and Anal Carcinomas
- Inflammatory mediators and NSAID effects
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Genetic factors in colorectal cancer
- Immune cells in cancer
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Microbial Community Ecology and Physiology
- Colorectal Cancer Screening and Detection
- Protist diversity and phylogeny
- Diagnosis and treatment of tuberculosis
- Ferroptosis and cancer prognosis
- Bioinformatics and Genomic Networks
- Microscopic Colitis
- Cancer, Stress, Anesthesia, and Immune Response
- COVID-19 Clinical Research Studies
- Gastric Cancer Management and Outcomes
- SARS-CoV-2 and COVID-19 Research
- Gene expression and cancer classification
- Historical Medical Research and Treatments
Université Paris Cité
2011-2024
Laboratoire d’immunologie intégrative du cancer
2013-2024
Hôpital Européen Georges-Pompidou
2014-2024
Inserm
2015-2024
Sorbonne Université
2011-2024
Assistance Publique – Hôpitaux de Paris
2011-2024
La Ligue Contre le Cancer
2020-2023
Centre de Recherche des Cordeliers
2009-2023
Délégation Paris 5
2009-2023
Hôpital Européen
2016-2022
The role of the adaptive immune response in controlling growth and recurrence human tumors has been controversial. We characterized tumor-infiltrating cells large cohorts colorectal cancers by gene expression profiling situ immunohistochemical staining. Collectively, immunological data (the type, density, location within tumor samples) were found to be a better predictor patient survival than histopathological methods currently used stage cancer. results validated two additional populations....
Abstract Summary: We have developed ClueGO, an easy to use Cytoscape plug-in that strongly improves biological interpretation of large lists genes. ClueGO integrates Gene Ontology (GO) terms as well KEGG/BioCarta pathways and creates a functionally organized GO/pathway term network. It can analyze one or compare two genes comprehensively visualizes grouped terms. A one-click update option allows automatically download the most recent GO/KEGG release at any time. provides intuitive...
The role of tumor-infiltrating immune cells in the early metastatic invasion colorectal cancer is unknown.
The tumor microenvironment includes a complex network of immune T-cell subpopulations. In this study, we systematically analyzed the balance between cytotoxic T cells and different subsets helper in human colorectal cancers correlated their impact on disease-free survival. A panel related genes were 125 frozen specimens. Infiltrating cells, Treg, Th1, Th17 also quantified center invasive margin tumors. By hierarchical clustering correlation matrix identified functional clusters associated...
Purpose The prognosis of patients with colorectal cancer has sometimes proved uncertain; thus, the prognostic significance immune criteria was compared that tumor extension using American Joint Committee on Cancer/International Union Against Cancer–TNM (AJCC/UICC-TNM) staging system. Patients and Methods We studied intratumoral infiltrates in center invasive margin 599 specimens stage I to IV cancers from two independent cohorts. analyzed these findings relation degree frequency recurrence....
Many patients who present with early-stage colorectal cancer (International Union Against Cancer TNM stages I and II) are nevertheless at high risk of relapse. We hypothesized that intratumoral immune reaction could influence their prognosis.The was investigated in 29 tumors by large-scale real-time polymerase chain reaction. Cytotoxic (CD8) memory (CD45RO) T cells were quantified immunohistochemical analyses tissue microarrays from the center (CT) invasive margin (IM) 602 two independent...
Local lymphatic vessel density and immune cytotoxicity prevent metastatic invasion.
While our knowledge about the roles of microbes and viruses in ocean has increased tremendously due to recent advances genomics metagenomics, research on marine microbial eukaryotes zooplankton benefited much less from these new technologies because their larger genomes, enormous diversity, largely unexplored physiologies. Here, we use a metatranscriptomics approach capture expressed genes open Tara Oceans stations across four organismal size fractions. The individual sequence reads cluster...
To determine whether the tumor immune infiltrate, as recently evaluated with Immunoscore methodology, could be a useful prognostic marker in patients rectal cancers.The influence of infiltrate on patient's outcome was investigated or without preoperative chemoradiation therapy (pCRT). The density total (CD3(+)) and cytotoxic (CD8(+)) T lymphocytes by immunohistochemistry quantified dedicated image analysis software surgical specimens cancer (n = 111) who did not receive pCRT biopsies...
A role for the immune system in controlling progression of solid tumors has been established several mouse models. However, effect responses and tumor escape on patient prognosis context human cancer is poorly understood. Here, we investigate cellular molecular parameters that could describe situ colorectal according to clinical metastatic lymph node or distant organ invasion (META- META+ patients). Primary samples carcinoma were analyzed by integrating large-scale phenotypic (flow...
Genomic alterations result in changes of immune cell densities within the tumor.
The purpose of this study was to evaluate the prognostic value Immunoscore in patients with stage III colon cancer (CC) and analyze its association effect chemotherapy on time recurrence (TTR).
Abstract Predicting responses of plankton to variations in essential nutrients is hampered by limited situ measurements, a poor understanding community composition, and the lack reference gene catalogs for key taxa. Iron driver dynamics and, therefore, global biogeochemical cycles climate. To assess impact iron availability on communities, we explored comprehensive bio‐oceanographic bio‐omics data sets from Tara Oceans context products two state‐of‐the‐art scale models. We obtained novel...
Abstract Purpose: No biomarker to personalize treatment in locally advanced rectal cancer (LARC) is currently available. We assessed LARC whether a diagnostic biopsy-adapted immunoscore (ISB) could predict response neoadjuvant (nT) and better define patients eligible an organ preservation strategy (“Watch-and-Wait”). Experimental Design: Biopsies from two independent cohorts (n1 = 131, n2 118) of with treated nT followed by radical surgery were immunostained for CD3+ CD8+ T cells quantified...
Background: The biological diagnostics of APS takes into account the persistent positivity for anticardiolipin and/or anti-β2GP1 antibodies presence lupus anticoagulant (LA). However, some new non-conventional antiphospholipid have emerged that could help in diagnosis APS. Objectives: To study potential usefulness clinical practice. Methods: 87 patients, aged from 15 to 92 years were included and classified following groups: 41 patients. positive conventional with criterion (31 primary 10...
Background New and fully validated tests need to be brought into clinical practice improve the estimation of recurrence risk in patients with colon cancer. The aim this study was assess analytical performances Immunoscore (IS) show its contribution prognosis prediction. Methods Immunohistochemical staining CD3+ CD8+ T cells on adjacent sections cancer tissues were quantified core tumor invasive margin dedicated IS modules integrated digital pathology software. Staining intensity across...
PURPOSE No biomarker capable of improving selection and monitoring patients with rectal cancer managed by watch-and-wait (W&W) strategy is currently available. Prognostic performance the Immunoscore biopsy (IS B ) was recently suggested in a preliminary study. METHODS This international validation study included 249 clinical complete response (cCR) W&W strategy. Intratumoral CD3+ CD8+ T cells were quantified on pretreatment biopsies digital pathology converted to IS . The primary end...
Background: The prognostic value of Immunoscore was evaluated in Stage II/III colon cancer (CC) patients, but it remains unclear I/II, and early-stage subgroups at risk. An international Society for Immunotherapy Cancer (SITC) study the pre-defined consensus tumors from 1885 AJCC/UICC-TNM I/II CC patients Canada/USA (Cohort 1) Europe/Asia 2). METHODS: Digital-pathology is used to quantify densities CD3+ CD8+ T-lymphocyte center tumor (CT) invasive margin (IM). time recurrence (TTR) primary...