A5041135887- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Polar Research and Ecology
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Aquaculture disease management and microbiota
- Chromosomal and Genetic Variations
- Viral gastroenteritis research and epidemiology
- Diatoms and Algae Research
- Marine and coastal ecosystems
- Plant Pathogens and Fungal Diseases
- Zoonotic diseases and public health
- Advanced Proteomics Techniques and Applications
- Marine Bivalve and Aquaculture Studies
- Microbial infections and disease research
- Microbial Metabolic Engineering and Bioproduction
- Biocrusts and Microbial Ecology
- Salmonella and Campylobacter epidemiology
- Genomic variations and chromosomal abnormalities
- Toxoplasma gondii Research Studies
- Advanced biosensing and bioanalysis techniques
Sorbonne Université
2017-2024
Centre National de la Recherche Scientifique
2015-2024
Laboratoire de Biodiversité et Biotechnologies Microbiennes
2024
Biologie Intégrative des Organismes Marins
2016-2023
Observatoire Océanologique de Banyuls-sur-Mer
2015-2021
Institut de Ciències del Mar
2019-2021
Pierre Fabre (France)
2018
UNSW Sydney
2010-2013
The University of Sydney
2010
Viruses are abundant ubiquitous members of microbial communities and in the marine environment affect population structure nutrient cycling by infecting lysing primary producers. Antarctic lakes microbially dominated ecosystems supporting truncated food webs which viruses exert a major influence on loop. Here we report discovery virophage (relative recently described Sputnik virophage) that preys phycodnaviruses infect prasinophytes (phototrophic algae). By performing metaproteogenomic...
Psychrophilic (cold-adapted) microorganisms make a major contribution to Earth's biomass and perform critical roles in global biogeochemical cycles. The vast extent environmental diversity of cold biosphere has selected for equally diverse microbial assemblages that can include archaea, bacteria, eucarya, viruses. Underpinning the important ecological psychrophiles are exquisite mechanisms physiological adaptation. Evolution also cold-active traits at level molecular adaptation, enzymes from...
Phytoplanktonic eukaryotes form huge panmictic populations and have evolved original hypervariability mechanisms to resist viral attack.
Abstract Predicting responses of plankton to variations in essential nutrients is hampered by limited situ measurements, a poor understanding community composition, and the lack reference gene catalogs for key taxa. Iron driver dynamics and, therefore, global biogeochemical cycles climate. To assess impact iron availability on communities, we explored comprehensive bio‐oceanographic bio‐omics data sets from Tara Oceans context products two state‐of‐the‐art scale models. We obtained novel...
Salmonella enterica serovar Typhimurium phage type 9 isolates resistant to streptomycin and sulfonamide have been recovered from both bovine human sources in Australia. This study aimed identify the resistance genes their location. Polymerase chain reaction was used screen for sul2 (sulphonamide resistance) strA strB (streptomycin were detected. A small plasmid carrying three these by transformation shown be essentially identical IncQ RSF1010. The sequences of one plasmid, pSRC15, RSF1010...
Virus-microbe interactions in the ocean are commonly described by "boom and bust" dynamics, whereby a numerically dominant microorganism is lysed replaced virus-resistant one. Here, we isolated microalga strain its infective dsDNA virus whose dynamics characterized instead parallel growth of both virus. Experimental evolution clonal lines revealed that this viral production originates from lysis minority virus-susceptible cells, which regenerated resistant cells. Whole-genome sequencing...
Mycobacterium strain NBB4 was isolated on ethene as part of a bioprospecting study searching for novel monooxygenase (MO) enzymes interest to biocatalysis and bioremediation. Previous work indicated that contained an unprecedented diversity MO genes, we hypothesized each type would support growth distinct hydrocarbon substrate. Here, attempted untangle the relationships between types substrates. Strain shown grow C2 -C4 alkenes -C16 alkanes. Complete gene clusters encoding six different...
While the molecular events involved in cell responses to heat stress have been extensively studied, our understanding of genetic basis basal thermotolerance, and particularly its evolution within green lineage, remains limited. Here, we present 13.3-Mb haploid genome transcriptomes a halotolerant thermotolerant unicellular alga, Picochlorum costavermella (Trebouxiophyceae) investigate genomic thermotolerance. Differential gene expression at high standard temperatures revealed that more...
Micro-algae of the genus Ostreococcus and related species order Mamiellales are globally distributed in photic zone world's oceans where they contribute to fixation atmospheric carbon production oxygen, besides providing a primary source nutrition food web. Their tiny size, simple cells, ease culture, compact genomes susceptibility most abundant large DNA viruses sea render them attractive as models for integrative marine biology. In spontaneous resistance occurs frequently. Here, we show...
Prasinoviruses are large DNA viruses that infect diverse genera of green microalgae worldwide in aquatic ecosystems, but molecular knowledge their life cycles is lacking. Several complete genomes both these and marine algal hosts now available have been used to show the pervasive presence species microbial metagenomes. We analyzed cycle Ostreococcus tauri virus 5 (OtV5), a lytic virus, using transcriptome sequencing (RNA-Seq) from 12 time points healthy or infected cells over day/night...
Members of the class Mamiellophyceae comprise species that can dominate picophytoplankton diversity in polar waters. Yet, are often morphologically indistinguishable from temperate species, although clearly separated by molecular features. Here we examine four strains Canadian Arctic. The 18S rRNA and Internal Transcribed Spacer 2 (ITS2) gene phylogeny place these within family Mamiellaceae (Mamiellales, Mamiellophyceae) two separate clades genus Mantoniella. ITS2 synapomorphies support...
Ostreococcustauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13-22 Mb genomes phylogenetically divergent species within this class are available, and their DNA sequences nearly always present metagenomic data produced from marine samples. Here we describe a simplified robust transformation protocol for the smallest these (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than...
One of the major challenges in viral ecology is to assess impact viruses controlling abundance specific hosts environment. To this end, techniques that enable detection and quantification virus–host interactions at single-cell level are essential. With goal mind, we implemented VirusFISH (Virus Fluorescence situ Hybridization) using as a model marine picoeukaryote Ostreococcus tauri its virus OtV5. allowed visualization fraction infected cells during an infection cycle experimental...
Ostreococcus is a cosmopolitan marine genus of phytoplankton found in mesotrophic and oligotrophic waters, the smallest free-living eukaryotes known to date, with cell diameter close 1 μm. has been extensively studied as model system investigate viral-host dynamics culture, yet impact viruses naturally occurring populations largely unknown. Here, we used Virus Fluorescence situ Hybridization (VirusFISH) visualize quantify natural during seasonal cycle central Cantabrian Sea (Southern Bay...
The genomic revolution has bridged a gap in our knowledge about the diversity, biology and evolution of unicellular photosynthetic eukaryotes, which bear very few discriminating morphological features among species from same genus. high-quality genome resources available class Mamiellophyceae (Chlorophyta) have been paramount to estimate diversity screen metagenomic data assess biogeography ecological niches different on global scale. Here we review current ecology large double-stranded DNA...
Viruses are a highly abundant, dynamic, and diverse component of planktonic communities that have key roles in marine ecosystems. We aimed to reveal the diversity dynamics large dsDNA viruses infecting algae Northern Skagerrak, South Norway through year by metabarcoding, targeting major capsid protein (MCP) its correlation protist dynamics. Metabarcoding results demonstrated high algal compared previous metabarcoding surveys Norwegian coastal waters. obtained 313 putative virus operational...
Prasinoviruses are large dsDNA viruses commonly found in aquatic systems worldwide, where they can infect and lyse unicellular prasinophyte algae such as Ostreococcus. Host susceptibility is virus strain-specific, but resistance of susceptible Ostreococcus tauri strains to a virulent arises frequently. In clonal resistant lines that re-grow, usually present for many generations, genes clustered on chromosome 19 show physical rearrangements differential expression. Here, we investigated...
Ostreococcus tauri is a simple unicellular green alga representing an ecologically important group of phytoplankton in oceans worldwide. Modern molecular techniques must be developed order to understand the mechanisms that permit adaptation microalgae their environment. We present for first time O. detailed characterization individual genomic integration events foreign DNA plasmid origin after PEG-mediated transformation. Vector occurred randomly at single locus genome and mainly as copy....
Driven by advances in DNA sequencing technologies, an astounding amount of data is being generated from genetic material sourced directly the environment, and this exponential growth set to continue. By surmounting challenges working with such vast datasets, a whole new level understanding gained about microbial diversity, evolution ecosystem function. For precious, pristine logistically difficult obtain Antarctic samples, metagenomic metaproteomic approaches are providing basis for...