A5100658760- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Cervical Cancer and HPV Research
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Catalytic Processes in Materials Science
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Catalysts for Methane Reforming
- Catalysis and Hydrodesulfurization Studies
- RNA Research and Splicing
- Pancreatic function and diabetes
- Lung Cancer Treatments and Mutations
- Advanced Proteomics Techniques and Applications
- Cardiac and Coronary Surgery Techniques
- Reproductive tract infections research
- Single-cell and spatial transcriptomics
- Cancer, Stress, Anesthesia, and Immune Response
- Metabolism, Diabetes, and Cancer
- Viral-associated cancers and disorders
- Antibiotic Resistance in Bacteria
- Macrophage Migration Inhibitory Factor
- Inflammatory Biomarkers in Disease Prognosis
Princess Margaret Cancer Centre
2018-2025
University Health Network
2018-2025
Fourth Hospital of Hebei Medical University
2020-2021
Hebei Medical University
2020-2021
Fudan University
2020
Cornell University
2019
Kingmed Diagnostics
2019
Guangzhou Medical University
2019
Southern Medical University
2014-2016
Nanfang Hospital
2016
Abstract Purpose: Circulating tumor DNA (ctDNA) enables personalized treatment strategies in oncology by providing a noninvasive source of clinical biomarkers. In patients with low ctDNA abundance, tumor-naïve methods are needed to facilitate implementation. Here, using locoregionally confined head and neck squamous cell carcinoma (HNSCC) as an example, we demonstrate detection simultaneous profiling mutations methylation. Experimental Design: We conducted CAncer Personalized Profiling deep...
Most cervical cancers are caused by human papilloma virus (HPV), and HPV circulating tumor DNA (ctDNA) may identify patients at highest risk of relapse. Our pilot study using digital polymerase chain reaction (dPCR) showed that detectable ctDNA the end chemoradiation (CRT) is associated with inferior progression-free survival (PFS) a next-generation sequencing approach (HPV-seq) outperform dPCR. We aimed to prospectively validate as tool for early detection residual disease.
Abstract Purpose: Early detection and intervention can decrease the mortality of breast cancer significantly. Assessments genetic/genomic variants in circulating tumor DNA (ctDNA) have generated great enthusiasm for their potential application as clinically actionable biomarkers management early-stage cancer. Experimental Design: In this study, 861 serial plasma matched tissue specimens from 102 patients with who need chemotherapy 50 individuals benign tumors were deeply sequenced via...
Despite intense multi-modal treatment, the prognosis for advanced cervical cancer remains poor. The recent increase in cases worldwide highlights an urgent need clinically validated biomarkers to guide patient management. Our pilot study investigates utility of human papillomavirus (HPV) circulating tumor deoxy ribonucleic acid (ctDNA) metastatic and recurrent cancer. We investigated association HPV ctDNA levels, early kinetics, detection viral-host integration sites with response outcomes....
Background: Human papillomavirus (HPV) DNA is detectable in the peripheral blood plasma from patients with locally advanced and metastatic cervical cancer. Levels of HPV circulating tumor (ctDNA) pretreatment have weak associations prognosis, but significance detecting integration into host genome or fragmentation features within ctDNA has not been explored. We hypothesized that these molecular may serve as prognostic biomarkers reflect biology, independent total abundance. Methods: Plasma...
Abstract Introduction: The incidence of human papillomavirus-positive (HPV+) oropharyngeal cancer (OPC) has increased rapidly, and HPV early antigen serology been proposed as a scalable cost-effective detection test. seropositivity can precede clinical presentation OPC by several years, so additional surveillance procedures may be necessary to optimize detection. potential for circulating tumor DNA (ctDNA) confirm diagnosis in seropositive individuals is poorly understood. Here, we assess...
Rationale: Interleukin 22 (IL-22) is an epithelial survival cytokine that at present being explored as therapeutic agents for acute and chronic liver injury. However, its molecular basis of protective activities remains poorly understood. Methods: Here we demonstrate IL-22 inhibits the deteriorating metabolic states induced by stimuli in hepatocytes. Utilizing cell biological, molecular, biochemical approaches, provide evidence promotes oxidative phosphorylation (OXPHOS) glycolysis regulates...
Pin2/TRF1 binding protein X1 (PinX1) has been identified as an endogenous telomerase inhibitor and a major haploinsufficient tumor suppressor gene. Increasing evidence suggests that reduced expression of PinX1 plays key role in tumorigenesis. However, the status its correlation with clinicopathological features prostate cancer (PCa) have not investigated.PinX1 mRNA PCa adjacent normal tissues were evaluated by real-time quantitative RT-PCR (qRT-PCR) western blotting. The significance was...
As a major tumor suppressor gene, the role of PinX1 in breast cancer and its molecular mechanism remain unclear. In this study, overexpression was generated 3 cell lines, knockdown performed nontumorigenic line. The regulation on proliferation cycle observed. A microarray-based lncRNA mRNA expression profile screening also performed. We found lower growth rate, G0/G1 phase arrest, S inhibition overexpressed cells, while higher decreased phase, increased rate knocked-down cell. total 977...
Aim: To explore the potential mechanism of synergetic effect α-lipoic acid with keratinocyte growth factor (KGF) on protecting alveolar epithelial type II cells (ATIICs) from hyperoxia-induced injury. Methods: Primary culture ATIICs Sprague-Dawley rat fetuses was examined under room air and 95% O2. Various KGF concentrations (0 to 100 ng/mL) 0.5 mM were added into cell culture. Levels intracellular reactive oxygen species, necrosis, proliferation measured using flow cytometry, ELISA, MTT...
Oral squamous cell carcinoma (OSCC) is one of the most common types cancer worldwide. Accumulating evidence has shown that long noncoding RNAs (lncRNAs) serve important roles in development OSCC. The purpose this study was to investigate biological function and underlying regulatory mechanism lncRNA homeobox A cluster antisense RNA2 (HOXA-AS2) RT-qPCR performed analyze HOXA-AS2 expressions human immortalized oral epithelial (HIOEC) line, OSCC lines, plasma. expression enhancer zeste 2...
Long non‑coding RNAs (lncRNAs) serve important roles in the tumorigenesis of a diverse range cancer types. The lung cancer‑associated transcript 1 (LUCAT1), has been reported to promote proliferation, migration and invasion oral squamous cell carcinoma cells. However, exact role LUCAT1 laryngeal (LSCC) remains fully understood. present study aimed interrogate modulatory mechanism LSCC. Reverse transcription‑quantitative PCR western blotting were used investigate expression miR‑493, as well...
Abstract Background: Human papillomavirus (HPV)-associated cancers often present with locoregionally confined disease and are treated curative intent. An emerging treatment paradigm includes radical therapy (i.e., chemoradiotherapy or surgery) followed by adjuvant treatment. Accurate detection of minimal residual (MRD) following could enable personalized use The HPV genome offers a convenient circulating tumor (ct)DNA marker for HPV-associated cancers, but current methods such as digital...
6065 Background: Despite intensive therapy, 30% of patients (pts) with LA-HNSCC relapse. Published data shows that MRD detection during follow up (FU) may predict relapse, e.g. human papillomavirus (HPV) DNA in p16+ pts post radiation (RT) or chemoradiation (CRT); circulating tumor (ctDNA) surgery. using multiple assays after definitive RT/CRT has not been reported. Methods: We enrolled high risk (stage III HPV+, III-IVB HPV-) treated curative intent: surgery ± adjuvant RT CRT. Plasma was...
(Abstracted from J Clin Oncol 2023;42:431–440 The majority of cervical cancers are caused by human papillomavirus (HPV), and despite treatment with chemoradiation (CRT), up to 40% patients locally advanced cancer will relapse. Current prognostic factors poor predictors relapse; however, detection HPV circulating tumor DNA (ctDNA) after CRT may identify those at higher risk
ABSTRACT β-Lactams are the most widely used antibiotics for treatment of bacterial infections because their proven track record safety and efficacy. However, susceptibility to β-lactam is continually eroded by resistance mechanisms. Emerging multidrug-resistant (MDR) Neisseria gonorrhoeae strains possessing altered penA alleles (encoding PBP2) pose a global health emergency as they threaten utility ceftriaxone, last remaining outpatient antibiotic. Here we disclose novel...
Objective To investigate the regulatory function of microRNA-330-3p (miR-330-3p) on cell growth and apoptosis in oral squamous carcinoma (OSCC) , to screen verify revelant target genes. Methods The expressions miR-330-3p were measured OSCC tissues, adjacent normal tissues lines by quantitative real-time PCR. Then inhibitor mimics transfected into line HN13 cells, their transfection efficiency was detected And then, proliferation assay, cloning experiments flow cytometry conducted...
Abstract Head and neck squamous cell carcinoma (HNSCC) comprises 3% of all cancer cases worldwide. Despite intensive multimodal therapies, HNSCC patient outcomes remain heterogeneous with minimal improvements in survival. Utilization fluid-based biomarkers for prognostication, risk stratification, disease surveillance may improve by enabling more effective treatment decisions. Here, we describe the performance comprehensive mutation methylome analysis highly sensitive detection circulating...