A5074616459- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Immune cells in cancer
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Cancer Cells and Metastasis
- interferon and immune responses
- Mesenchymal stem cell research
- IL-33, ST2, and ILC Pathways
- RNA modifications and cancer
- Occupational and environmental lung diseases
- Lymphoma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Pleural and Pulmonary Diseases
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Cutaneous lymphoproliferative disorders research
- Adipokines, Inflammation, and Metabolic Diseases
- Histone Deacetylase Inhibitors Research
Inserm
2015-2025
Université de Rennes
2017-2024
Établissement Français du Sang
2019-2024
La Ligue Contre le Cancer
2023-2024
Centre Hospitalier Universitaire de Rennes
2022
StemCells (United States)
2020
Université Rennes 2
2020
Cancéropôle Grand Ouest
2019
Princess Margaret Cancer Centre
2015-2018
University Health Network
2015-2018
Significance TLS, which are clusters of lymphocytes and stromal cells observed at sites chronic inflammation, play a key role in sustaining disease progression autoimmune conditions. While the these structures has been studied extensively, fibroblasts, nonhematopoietic cells, formation maintenance TLS not demonstrated. Here, we establish that, establishment, resident fibroblasts expand acquire immunological features process that is dependent on IL13 IL22. Interference with this or depletion...
Plasmacytoid dendritic cells (pDC) are antigen-presenting specialized in antiviral response. The measles virus vaccine is proposed as an antitumor agent to target and specifically kill tumor without infecting healthy cells.Here, we investigated, vitro, the effects of vaccine-infected on phenotype functions human pDC. We studied maturation antigen cross-presentation by pDC, exposed either alone, or UV-irradiated cells.We found that only induced pDC with a strong production IFN-α, whereas were...
Abstract Clinical-grade mesenchymal stromal cells (MSCs) can be expanded from bone marrow and adipose tissue to treat inflammatory diseases degenerative disorders. However, the influence of their origin on functional properties, including immunosuppressive activity, remains unsolved. In this study, we produced paired marrow-derived cell (BM-MSC) adipose-derived (ASC) batches 14 healthy donors. We then compared them using transcriptomic, phenotypic, analyses validated our results purified...
// Carole Achard 1,2,3 , Nicolas Boisgerault Tiphaine Delaunay David Roulois Steven Nedellec 3,4 Pierre-Joseph Royer 3,5 Mallory Pain Chantal Combredet 6 Mariana Mesel-Lemoine Laurent Cellerin 7 Antoine Magnan 3,5,8 Frédéric Tangy Marc Grégoire and Jean-François Fonteneau 1 INSERM, UMR892, Institut de Recherche en Santé l'Université Nantes, France 2 CNRS, UMR6299, 3 Université 4 INSERM UMS016, SFR Santé, 5 UMRS1087, du Thorax, CNRS UMR3569, Unité Génomique Virale et Vaccination, Pasteur,...
Tertiary lymphoid structures and aggregates are reported in the meninges of patients with multiple sclerosis (MS), especially at progressive stage, strongly associated cortical lesions disability. Besides B cells, these comprise follicular helper T (Tfh) cells that crucial to support B-cell differentiation. Tfh play a pivotal role amplifying autoreactive promoting autoantibody production several autoimmune diseases, but very few known MS. In this study, we examined phenotype, frequency,...
Bone marrow (BM) involvement is a common feature of germinal center-derived B cell lymphomas and associated with poor prognosis. In particular, follicular lymphoma (FL) infiltrates the BM in 70% cases, analysis vitro-expanded FL mesenchymal stromal cells (MSCs) has revealed an extensive alteration phenotypic, transcriptomic, functional profiles. However, mechanisms underlying direct interplay between their permissive niche situ have not been yet identified. current work, we identified...
Recent studies have demonstrated that DNA demethylation agents can mimic a viral infection by induction of dsRNAs. This mimicry leads to an antiviral response mediated the cytosolic pattern recognition receptor MDA5, followed MAVS (IPS1) activation, IRF7 nuclear translocation and upregulation type III Interferon interferon-stimulated genes.
// Aline C. Planello 1,2,* , Rajat Singhania 1,* Ken J. Kron 1 Swneke D. Bailey David Roulois Mathieu Lupien 1,3 Sérgio R. Peres Line 2 Ana Paula de Souza and Daniel De Carvalho Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada Department of Morphology, Piracicaba Dental School, Campinas, Piracicaba, SP, Brazil 3 Medical Biophysics, * These authors have contributed equally to this work Correspondence to: Carvalho, email: Souza, Keywords : DNA methylation,...
// David Roulois 1, 2, 3 , Sophie Deshayes Marie-Noëlle Guilly 4 Joëlle S. Nader Charly Liddell Myriam Robard 5 Philippe Hulin Amal Ouacher Vanessa Le Martelot Jean-François Fonteneau Marc Grégoire Christophe Blanquart 3, * Daniel L. Pouliquen 1 CRCNA, Université d'Angers, de Nantes, France 2 INSERM, CNRS, CEA, DSV, iRCM, LCE, BP6, Fontenay aux Roses cedex, Cellular and Tissular Imaging Core Facility (MicroPICell), These authors contributed equally to this work Correspondence to: Pouliquen,...
Recent clinical investigations have demonstrated that T-cell-based immunotherapy of malignant pleural mesothelioma (MPM) could represent an alternative to the other therapeutic strategies. However, its development suffers from lack identified tumour antigenic targets. Mucin (MUC)1, which is expressed and recognised by cytotoxic T-cells in numerous cancer types, has not been investigated as a potential immune target MPM. Thus, objective this study was analyse MUC1 expression MPM cells...
Research into new treatments against malignant pleural mesothelioma (MPM) is of great interest, as this aggressive cancer often resistant to conventional therapies. One potential strategy the use epigenetic drugs, such 5-aza-2'-deoxycytidine (5-azaCdR), a DNA-hypomethylating drug, and valproate (VPA), histone deacetylase inhibitor (HDACi). Indeed, these drugs not only trigger MPM cell death, but also induce expression testis antigens recognized by CD8(+) T cells, New York-esophageal cancer-1...
Mature lymphoid stromal cells (LSCs) are key organizers of immune responses within secondary organs. Similarly, inflammation-driven tertiary structures depend on immunofibroblasts producing cytokines and chemokines. Recent studies have explored the origin heterogeneity LSC/immunofibroblasts, yet molecular epigenetic mechanisms involved in their commitment still unknown. This study transcriptomic reprogramming underlying LSC/immunofibroblast commitment. We identified induction lysine...
Abstract Tumor endothelial (TECs) cells play a critical role in regulating immune responses within the tumor microenvironment (TME). However, mechanisms by which TECs modulate cell population remain unclear, particularly non-small lung cancer (NSCLC). Here, we investigated how NSCLC tweak normal (NECs) into and subsequent effects on regulation. NECs were cocultured with various lines, using 2D 3D coculture models to evaluate TEC-mediated cells. We show that direct led significant...
Abstract Recent studies have shown that DNA methyltransferase inhibitors (DNMTi) can induce IRF7 activation and Type I/III interferon signaling through dsRNA-mediated viral mimicry in cancer cells. By performing a large pan-cancer analysis using TCGA data, we determined is associated with higher CD8+ T cell tumor infiltration cytolytic activity across multiple types. Accordingly, demonstrate DNMTi treatment results increased infiltration, enhanced dependent growth inhibition. Finally, show...
In B lymphocytes, class switch recombination (CSR) is an essential process that adapts immunoglobulin (Ig) subtypes to antigen response. Taking place within the Ig heavy chain (IgH) locus, CSR needs controlled transcription of targeted regions governed by IgH 3' regulatory region (3'RR). This super-enhancer composed four core enhancers surrounded inverted repeated sequences, forming a quasi-palindrome. addition transcription, nuclear organization appears be important level in regulation....