A5068409181- Oral microbiology and periodontitis research
- Oral Health Pathology and Treatment
- Protease and Inhibitor Mechanisms
- Epigenetics and DNA Methylation
- Dental materials and restorations
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Bone and Dental Protein Studies
- Aquaculture disease management and microbiota
- Dental Implant Techniques and Outcomes
- dental development and anomalies
- Endodontics and Root Canal Treatments
- Oral and gingival health research
- Pediatric health and respiratory diseases
- Dental Erosion and Treatment
- SARS-CoV-2 and COVID-19 Research
- Cleft Lip and Palate Research
- HIV/AIDS oral health manifestations
- Periodontal Regeneration and Treatments
- Cancer-related gene regulation
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Hedgehog Signaling Pathway Studies
- DNA and Nucleic Acid Chemistry
Hospital Moinhos de Vento
2021-2025
Universidade Estadual de Campinas (UNICAMP)
2012-2023
Universidade Estadual Paulista (Unesp)
2016-2018
Manchester Metropolitan University
2015
Division of Chemistry
2015
Universidade do Estado do Pará
2015
Morpho (United States)
2013
MRC Laboratory of Molecular Biology
2009-2010
Universidade Sagrado Coração
2003-2007
Methodist University of Piracicaba
2000-2005
Abstract Background: Interleukin‐6 (IL‐6) is a multifunctional cytokine that mediates inflammatory tissue destruction. A G to C substitution at position −174 in the promoter of IL‐6 gene reduces vitro transcription IL‐6. This polymorphism has been associated with diseases like chronic arthritis. Objective: The aim this study was investigate association between and susceptibility periodontitis Brazilians. Material Methods: Eighty‐four nonsmoking subjects over 25 years (mean age 42.4) were...
Abstract Background: Chronic periodontitis (CP) is characterized by an inflammation in the supporting tissues of teeth caused primarily bacterial infection. Interleukin 10 (IL10) anti‐inflammatory cytokine whose genetic polymorphisms may influence expression protein. Objective: In this study we investigated hypothesis that single‐nucleotide (SNPs) promoter IL10 gene might be related to CP. Materials and Methods: DNA was obtained from n =67 CP patients =43 control subjects. All studied...
Abstract Background: A single nucleotide polymorphism was described in the promoter region of human MMP‐1 gene, and this has been associated with risk cancer metastasis inflammatory diseases. In paper, we studied possible relationship between severity chronic periodontitis. Methods: Genomic DNA from oral mucosa amplified by PCR analyzed restriction endonuclease. The alleles were separated polyacrylamide gel electrophoresis. significance differences observed frequencies moderate severe...
Background: Genetic polymorphisms in the vitamin D receptor (VDR) gene are associated with bone homeostasis and diseases which loss is a cardinal sign. The aim of this study was to determine whether chronic periodontal disease Brazilian population VDR gene. Methods: Clinical examination recordings probing depth clinical attachment level were performed 113 unrelated adults who divided into two groups: 44 healthy individuals (control group) 69 subjects periodontitis (CP). DNA obtained from...
This study analysed the status of DNA methylation in promoter region IL8 gene oral mucosa cells from healthy, smoker and non-smoker subjects with chronic periodontitis compared these findings among groups mRNA levels.Genomic epithelial 41 healthy subjects, 30 smokers 40 non-smokers were purified modified by sodium bisulphite. Genomic blood leucocytes gingival biopsies 13 each group also Modified was submitted methylation-specific polymerase chain reaction (PCR) (MSP), electrophoresed on 10%...
We question the utilisation of<italic>in situ</italic>metallic modified electrodes towards detection of lead(<sc>ii</sc>) and cadmium(<sc>ii</sc>) showing that comparable electroanalytical responses are achieved at bare/unmodified counterparts.
Abstract Background: Interleukin‐2 (IL‐2) is a pro‐inflammatory cytokine derived from Th1 cells. This involved in B‐cell activation and stimulates macrophages, natural killer cells, T‐cell proliferation osteoclast activity. IL‐2 has been also implicated the stimulation of activity bone resorption. Objective: In this study relationship between polymorphism − 330 (T→G) gene different levels chronic periodontal disease was investigated. Material Methods: DNA extracted buccal epithelial cells...
Abstract Objectives: Matrix metalloproteinase‐1 and ‐3 (MMP‐1, MMP‐3) represent proteinases that degrade macromolecules of the extracellular matrix. These enzymes play a fundamental role during destruction periodontal tissues. Genetic polymorphisms were characterized in promoter region MMP‐1 MMP‐3 genes. The aim this study was to investigate relationship between these genetic variations with chronic periodontitis Brazilian population. Material Methods: Non‐smoking subjects ( n =114)...
Background: Studies evaluating the methylation status of cytokine genes may have relevance for inflammatory diseases in which expression some cytokines is altered, such as periodontitis. This study observes DNA interleukin‐8 (IL8) gene promoter cells oral epithelium subjects affected by generalized aggressive periodontitis (AgP) and compares it to those control subjects. Methods: Genomic from epithelial 37 AgP patients controls were purified modified sodium bisulphite. Modified was submitted...
Background: The proinflammatory chemokine interleukin (IL)‐8 is important in the regulation of inflammatory response. Analyses single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that A allele upregulated IL‐8 levels after stimulation with lipopolysaccharides. We investigated association SNP rs4073 chronic periodontitis. Methods: Genotyping was performed by a standard polymerase chain reaction–restriction fragment length assay 289 genomic DNA samples healthy control...
De Oliveira NFP, Andia DC, Planello AC, Pasetto S, Marques MR, Nociti FH, Line SRP, Souza, AP. TLR2 and TLR4 gene promoter methylation status during chronic periodontitis. J Clin Periodontol 2011; 38: 975–983. doi: 10.1111/j.1600‐051X.2011.01765.x Abstract Aim: The objective of this study was to analyse the DNA in region toll‐like receptor ( TLR ) 2 genes gingival tissue samples from healthy subjects, smokers non‐smokers affected by Material Methods: Genomic total RNA were purified using...
Matrix metalloproteinases (MMPs) play a key role in the tissue destruction characteristic of chronic periodontitis. The purpose this study was to investigate association MMP and TIMP polymorphisms with periodontitis two populations.A total 34 spanning 12 2 genes were genotyped 401 individuals from Brazil (99 cases 302 controls), 274 US (70 204 controls). Individuals considered if presenting at least three teeth exhibiting sites clinical attachment loss ≥ 5 mm different quadrants. Controls...
Abstract Background: Matrix metalloproteinases (MMP)‐9 is an important member of the matrix metalloproteinase family. A functional polymorphism has been described in promoter region human MMP‐9 gene. C‐to‐T base exchange at −1562 creates two different alleles, and C/T T/T genotypes promote high activity gene promoter, increasing risk for inflammatory diseases. The metalloproteinase‐2 tissue inhibitor (TIMP‐2) regulates MMPs extracellular matrix, a −418 position TIMP‐2 found Sp‐1 binding...
Chronic periodontitis represents a complex disease that is hard to control and not completely understood. Evidence from past studies suggests there key role for DNA methylation in the pathogenesis of periodontitis. However, all reports have applied technologies investigate genes low throughput. In order advance knowledge disease, we analyzed variations associated with gene transcription using high-throughput assay. Infinium® HumanMethylation450 (Illumina) was performed on gingival samples 12...
The development of vascular neurology as the primary specialty for stroke evaluation and treatment is a relatively recent advancement. Vascular well-established in United States, ensuring most patients receive specialized care, but many globally are managed by physicians without training cerebrovascular neurological medicine that partly manifested availability institutions incorporate dedicated training. Our goal to evaluate providers worldwide identify who leading care different regions,...
Access to acute stroke care varies widely worldwide, with significant gaps in low- and middle-income countries rural areas. Ensuring equitable access requires a standardized approach identify current coverage potential sites for new centers. In the U.S., center distribution is inconsistent across states, around 77% of counties deemed "medical deserts" due lack healthcare services. Methods: We conducted comprehensive survey hospitals Oklahoma assess accessibility therapies. gathered data on...
Abstract Background: Cytokines are key factors that mediate the inflammatory process during periodontal disease. Recent works have shown levels of cytokine expression regulated by genetic polymorphisms, and these variations can interfere with progression The−590 (C→T) polymorphism IL4 gene is associated high IgE in asthmatic families, frequency T allele was increased children. The concentration gingival tissue found to be elevated patients periodontitis. Objective: In this study relationship...