Buccal cells provide a convenient source of DNA for epidemiological studies. The goal this study was to develop method obtain buccal from mouthwash samples be used as DNA, and evaluate the stability in solution over time. procedures described paper avoid use any organic solvents. This is achieved by salting out cellular proteins dehydration precipitation with saturated ammonium acetate solution. protocol here fast, simple perform, sensitive, economical several can processed at same analyses...

Abstract Background: Interleukin‐6 (IL‐6) is a multifunctional cytokine that mediates inflammatory tissue destruction. A G to C substitution at position −174 in the promoter of IL‐6 gene reduces vitro transcription IL‐6. This polymorphism has been associated with diseases like chronic arthritis. Objective: The aim this study was investigate association between and susceptibility periodontitis Brazilians. Material Methods: Eighty‐four nonsmoking subjects over 25 years (mean age 42.4) were...

Abstract Background: Chronic periodontitis (CP) is characterized by an inflammation in the supporting tissues of teeth caused primarily bacterial infection. Interleukin 10 (IL10) anti‐inflammatory cytokine whose genetic polymorphisms may influence expression protein. Objective: In this study we investigated hypothesis that single‐nucleotide (SNPs) promoter IL10 gene might be related to CP. Materials and Methods: DNA was obtained from n =67 CP patients =43 control subjects. All studied...

Tooth enamel is the hardest, densest and most mineralized tissue in vertebrates.

Abstract Background: A single nucleotide polymorphism was described in the promoter region of human MMP‐1 gene, and this has been associated with risk cancer metastasis inflammatory diseases. In paper, we studied possible relationship between severity chronic periodontitis. Methods: Genomic DNA from oral mucosa amplified by PCR analyzed restriction endonuclease. The alleles were separated polyacrylamide gel electrophoresis. significance differences observed frequencies moderate severe...

Abstract The interaction of the murine monoclonal anti‐DNA antibody H241 with extracellular glomerular antigens was found to be due binding this laminin, major non‐collagenous protein constituent basement membrane. This is specific, since it inhibited by double‐stranded DNA and single‐stranded in solution. Furthermore, mouse laminin mediated conformational properties antigen because mild denaturation strongly decreases capacity H241, while exposure sodium dodecyl sulfate, completely...

This study analysed the status of DNA methylation in promoter region IL8 gene oral mucosa cells from healthy, smoker and non-smoker subjects with chronic periodontitis compared these findings among groups mRNA levels.Genomic epithelial 41 healthy subjects, 30 smokers 40 non-smokers were purified modified by sodium bisulphite. Genomic blood leucocytes gingival biopsies 13 each group also Modified was submitted methylation-specific polymerase chain reaction (PCR) (MSP), electrophoresed on 10%...

<b><i>Objective:</i></b> To analyze macroscopic, microscopic, and ultrastructural aspects of enamel from head-and-neck cancer patients submitted to radiotherapy. <b><i>Materials Methods:</i></b> Twenty sound extracted permanent molars were used divided into 2 groups. The experimental group consisted 10 radiotherapy with total doses that ranged 50 70 Gy. Ten who did not receive matched experimental-group samples by anatomic tooth comprised the...

SUMMARY A major question in modern biology is how gene mutations affect development and are translated into macroevolutionary changes morphology. Variations tooth number, a strategy used by many mammals to develop specialized dentitions, has been an important factor for species diversification. Changes the number of teeth tend occur reverse order formed during development, which also characterizes general pattern loss observed evolution placental mammals. To understand at molecular level...

Abstract Background: Interleukin‐2 (IL‐2) is a pro‐inflammatory cytokine derived from Th1 cells. This involved in B‐cell activation and stimulates macrophages, natural killer cells, T‐cell proliferation osteoclast activity. IL‐2 has been also implicated the stimulation of activity bone resorption. Objective: In this study relationship between polymorphism − 330 (T→G) gene different levels chronic periodontal disease was investigated. Material Methods: DNA extracted buccal epithelial cells...

Abstract Objectives: Matrix metalloproteinase‐1 and ‐3 (MMP‐1, MMP‐3) represent proteinases that degrade macromolecules of the extracellular matrix. These enzymes play a fundamental role during destruction periodontal tissues. Genetic polymorphisms were characterized in promoter region MMP‐1 MMP‐3 genes. The aim this study was to investigate relationship between these genetic variations with chronic periodontitis Brazilian population. Material Methods: Non‐smoking subjects ( n =114)...

Background: Studies evaluating the methylation status of cytokine genes may have relevance for inflammatory diseases in which expression some cytokines is altered, such as periodontitis. This study observes DNA interleukin‐8 (IL8) gene promoter cells oral epithelium subjects affected by generalized aggressive periodontitis (AgP) and compares it to those control subjects. Methods: Genomic from epithelial 37 AgP patients controls were purified modified sodium bisulphite. Modified was submitted...

Background: The proinflammatory chemokine interleukin (IL)‐8 is important in the regulation of inflammatory response. Analyses single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that A allele upregulated IL‐8 levels after stimulation with lipopolysaccharides. We investigated association SNP rs4073 chronic periodontitis. Methods: Genotyping was performed by a standard polymerase chain reaction–restriction fragment length assay 289 genomic DNA samples healthy control...

De Oliveira NFP, Andia DC, Planello AC, Pasetto S, Marques MR, Nociti FH, Line SRP, Souza, AP. TLR2 and TLR4 gene promoter methylation status during chronic periodontitis. J Clin Periodontol 2011; 38: 975–983. doi: 10.1111/j.1600‐051X.2011.01765.x Abstract Aim: The objective of this study was to analyse the DNA in region toll‐like receptor ( TLR ) 2 genes gingival tissue samples from healthy subjects, smokers non‐smokers affected by Material Methods: Genomic total RNA were purified using...

CpG islands (CGI) are genomic regions associated with gene promoters and involved in expression regulation. Despite their high content unlike bulk DNA methylation pattern, these usually hypomethylated. So far, the mechanisms controlling CGI patterning remain unclear. G-quadruplex (G4) structures can inhibit methyltransferases 1 enzymatic activity, leading to hypomethylation. Our aim was analyse association of G4 forming sequences (G4FS) as well determine intrinsic extrinsic characteristics...