A5100655179- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Thermal and Kinetic Analysis
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Energetic Materials and Combustion
- Chemical Thermodynamics and Molecular Structure
- Anodic Oxide Films and Nanostructures
- Metal Extraction and Bioleaching
- Crystal Structures and Properties
- Nuclear materials and radiation effects
- Luminescence Properties of Advanced Materials
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Iron and Steelmaking Processes
- Metal Alloys Wear and Properties
- Minerals Flotation and Separation Techniques
- Cancer-related molecular mechanisms research
- SARS-CoV-2 detection and testing
- Nanoparticles: synthesis and applications
- SARS-CoV-2 and COVID-19 Research
Shaanxi Normal University
2017-2025
Nanjing University
2023-2025
University of Science and Technology Beijing
2011-2024
Institute of Process Engineering
2011-2024
Ningxia Hui Autonomous Region Peoples Hospital
2024
China Jiliang University
2023-2024
Harbin Medical University
2023
Second Affiliated Hospital of Harbin Medical University
2023
Shanghai Jiao Tong University
2012-2022
Shanghai First People's Hospital
2022
Simultaneous profiling of multiple molecules trafficking at a single organelle and the surrounding cytosol within living cell is crucial for elucidating their functions, necessitating advanced techniques that provide high spatial–temporal resolution molecule specificity. In this study, we present an electrochemical nanodevice based on θ-nanopipette designed to coanalyze calcium ions (Ca 2+ ) reactive oxygen species (ROS) mitochondrion its cytosol, thereby enhancing our understanding...
Staphylococcus aureus can adhere to most foreign materials and form biofilm on the surface of medical devices. Biofilm infections are difficult resolve. The goal this in vitro study was explore use chitosan-coated nanoparticles prevent formation. For purpose, S. seeded 96-well plates incubate with iron oxide order efficiency formation inhibition. bacteria count determined using spread plate method; biomass measured crystal violet staining method. Confocal laser scanning microscopy electron...
Abstract Despite the successful application of upconversion nanoparticles (UCNPs), their low energy transfer efficiency is still a bottleneck to further applications. Here we design UCNPs with multilayer structure, including an inert NaYF 4 :Gd core and energy‐concentrating zone (ECZ), for efficient concentration. The ECZ composed emitting layer :Yb,Er absorption :Nd,Yb antenna IRDye 800CW manipulate transfer. stable tight packing linked originally bisphosphonate ligand improves greatly...
Chimeric peptide-DNAzyme (CPDzyme) is a novel artificial peroxidase that relies on the covalent assembly of DNA, peptides, and an enzyme cofactor in single scaffold. An accurate control these different partners allows for design CPDzyme prototype G4-Hemin-KHRRH, found to be >2000-fold more active (in terms conversion number kcat) than corresponding but non-covalent G4/Hemin complex and, importantly, >1.5-fold native (horseradish peroxidase) when considering catalytic center. This unique...
In the deoxyribonucleic acid (DNA) repair pathways, DNA enzymes have great significance for genomic integrity. As one important initiator of base-excision pathway, aberrant activity uracil-DNA glycosylase (UDG) is closely associated with many diseases. Herein, we developed a simple distance-based device visual detection UDG using load-free hydrogel. The hydrogel consists polyacrylamide–DNA chains being bridged by single-stranded crosslinker containing responsive uracil base site. can...
The target-dependent endonuclease activity (also known as the trans-cleavage activity) of CRISPR-Cas systems has stimulated great interest in development nascent sensing strategies for nucleic acid diagnostics. Despite many attempts, majority sensitive diagnostics mainly rely on preamplification, which generally needs complex probes/primers designs, multiple experimental steps, and a longer testing time, well introducing risk false-positive results. In this work, we propose CRISPR-Cas-Driven...
An ultrasensitive flow cytometric immunoassay (TdT-FCI) is developed based on bead surface-initiated template-free DNA extension.
G-quadruplex (G4)/hemin DNAzyme is a promising candidate to substitute horseradish peroxidase in biosensing systems, especially for the detection of nucleic acids. However, relatively suboptimal catalytic capacity limits its potential applications. This makes it imperative develop an ideal signal construction highly sensitive platforms. Herein, we integrated novel chimeric peptide-DNAzyme (CPDzyme) with ligase chain reaction (LCR) cost-efficient and By employing microRNA (miRNA)...
Disease diagnostics and surveillance increasingly highlight the importance of portable, cost-effective, sensitive point-of-care (POC) detection nucleic acids. Here, we report a CRISPR/Cas13a-responsive RNA-bridged DNA hydrogel capillary sensor for direct visual specific RNA with high sensitivity. The was simply prepared by loading RNA-cross-linking film (∼0.2 mm ± 0.02 mm) at end capillary. When CRISPR/Cas13a specifically recognizes target RNA, bridge in is cleaved trans-cleavage activity...
Nanomaterials excel in mimicking the structure and function of natural enzymes while being far more interesting terms structural stability, functional versatility, recyclability, large-scale preparation. Herein, story assembles hemin, histidine analogs, G-quadruplex DNA a catalytically competent supramolecular assembly referred to as assembly-activated hemin enzyme (AA-heminzyme). The catalytic properties AA-heminzyme are investigated both silico (by molecular docking quantum chemical...
Despite the unique advantage of isothermal exponential amplification reaction (EXPAR) for rapid detection short nucleic acids, it severely suffers from drawback sequence-dependent bias, mainly arising secondary structures EXPAR template under commonly used temperature (55 °C). As such, limits (LOD) different target sequences may vary considerably aM to nM. Here we report a sequence-generic (SG-EXPAR) that eliminates bias and achieves similar performance targets with generally sub-fM LODs....
Abstract Despite the successful application of upconversion nanoparticles (UCNPs), their low energy transfer efficiency is still a bottleneck to further applications. Here we design UCNPs with multilayer structure, including an inert NaYF 4 :Gd core and energy‐concentrating zone (ECZ), for efficient concentration. The ECZ composed emitting layer :Yb,Er absorption :Nd,Yb antenna IRDye 800CW manipulate transfer. stable tight packing linked originally bisphosphonate ligand improves greatly...
A modular single bead enrich-amplify-amplify strategy is proposed for simultaneous detection of multiple proteins at the aM level.
Strip shape control is a hotspot and challenge in strip rolling, where the development trend of rolling technology towards high strength, toughness, large width-to-thickness ratio. The influence material microstructure evolution on being increasingly emphasized. In this paper, Nb-Ti microalloyed steel taken as research object. Thermodynamic kinetic models focusing precipitation austenite phase are established to quantify process. A coupled model rolls strips built using ABAQUS 2022 software,...
Abstract Carbon monoxide (CO) functions as a significant endogenous cell signaling molecule and is strongly associated with many physiological pathological processes. However, conventional fluorescence imaging in the visible near‐infrared (NIR) I regions suffers autofluorescence background photon scattering, hindering accurate detection of CO vivo. In addition, complexity environments leads to fluctuating emission. To solve these problems, herein, NIR‐II fluorescent nanoprobe NP‐Pd for vivo...
Amplification-free and ultrasensitive detection of telomerase activity at the single-cell level via Cas12a-lighting-up single microbeads (Cas12a-LSMBs).