Abstract Background Glandular trichomes produce a wide variety of commercially important secondary metabolites in many plant species. The most prominent anti-malarial drug artemisinin, sesquiterpene lactone, is produced glandular Artemisia annua . However, only limited genomic information currently available this non-model Results We present global characterization A. trichome transcriptome using 454 pyrosequencing. Sequencing runs two normalized cDNA collections from yielded 406,044...

The solvothermal reaction of Mn(ClO4)2, NaN3, and a rigid viologen-tethered tetracarboxylic acid (1,1′-bis(3,5-dicarboxyphenyl)-4,4′-bipyridinium chloride, [H4L]Cl2) led to coordination polymer formula [Mn4(L)(N3)6(H2O)2]n. X-ray analysis revealed 3D structure. Mn(II) ions are connected by mixed azide carboxylate bridges give 2D layers, which pillared the viologen tether zwitterionic ligand. Magnetic analyses suggested that compound features antiferromagnetism field-induced metamagnetism....

RNAi-elicited gene silencing is heritable and can persist for multiple generations after its initial induction in C. elegans. However, the mechanism by which parental-acquired trait-specific information from RNAi inherited progenies not fully understood. Here, we identified a cytoplasmic Argonaute protein, WAGO-4, necessary inheritance of RNAi. WAGO-4 exhibits asymmetrical translocation to germline during early embryogenesis, accumulates at perinuclear foci germline, required exogenous...

Mn(II) and Fe(II) compounds derived from azide the zwitterionic 1-carboxylatomethylpyridinium-4-carboxylate ligand are isomorphous three-dimensional metal–organic frameworks (MOFs) with sra net, in which metal ions connected into anionic chains by mixed (μ-1,1-azide)bis(μ-carboxylate) triple bridges cross-linked cationic backbones of ligands. The MOFs display typical one-dimensional antiferromagnetic behavior. In contrast, one more d electron per center, counterpart shows intrachain...

Two Cd(ii) metal-organic frameworks were synthesized from the NH-functionalized dicarboxylate ligand 9H-carbazole-2,7-dicarboxylic acid (2,7-H2CDC). Compound , [Cd4(CDC)4(DMF)4]·4DMF·4H2O, displays 2D square grid networks based on novel tetranuclear [Cd4(COO)8] secondary building units (SBUs) and pairwise CDC(2-) linkers. (H3O)2[Cd3(2,7-CDC)4]·3DMF·4H2O, is also 4-connected SBUs CDC linkers, but unusual trinuclear [Cd3(COO)8] lead to 2-fold interpenetrated 3D diamond-type with guest...

Ribosome biogenesis is a multistep process, during which mistakes can occur at any step of pre-rRNA processing, modification, and ribosome assembly. Misprocessed rRNAs are usually detected degraded by surveillance machineries. Recently, we identified class antisense ribosomal siRNAs (risiRNAs) that down-regulate pre-rRNAs through the nuclear RNAi pathway. To further understand biological roles risiRNAs, conducted both forward reverse genetic screens to search for more suppressor siRNA ( susi...

Gene segments from other organisms, such as viruses, are detected foreign and targeted for silencing by RNAi pathways. A deep-sequencing map of the small RNA response to repeated transgenes introduced Caenorhabditis elegans revealed that specific siRNAs. Silencing gene depends on an antiviral involves changes in active silent chromatin modifications altered levels antisense Distinct Argonaute proteins target genes or protection against silencing. We used a transgene genome-wide screen...

An ultrasensitive flow cytometric immunoassay (TdT-FCI) is developed based on bead surface-initiated template-free DNA extension.

A new azido-functionalized Zr(<sc>iv</sc>) MOF was synthesized and further functionalized<italic>via</italic>post-synthetic click reactions, the amino-tagged is a base catalyst for Knoevenagel condensation.

A versatile single magnetic nanoparticle (MNP)-confined, click chemistry-actuated digital DNA walker (ddWalker) machine is devised for the absolute quantification of microRNA (miRNA). This delicate ddWalker allows one target molecule to fix walking leg on a MNP, following Poisson statistics through specific chemical ligation, which will initiate stepwise cleave molecular beacon tracks strictly constrained leg-hold MNP without cross-particle reaction, fluorescently "lighting up" exact MNP....

MicroRNAs (miRNAs) have been considered as promising cancer biomarkers. However, the simple but sensitive detection of low levels miRNAs in biological samples still remains challenging. Herein, we wish to report an entirely enzyme-free, simple, and highly miRNA assay based on counting cycling click chemical ligation (3CL)-illuminated fluorescent magnetic nanoparticles (MNPs) with a total internal reflection fluorescence microscopy (TIRFM). In this strategy, each molecule can trigger many...

Vacancy defects caused by doping are beneficial to optimizing the regional electronic structure and increasing exposed active sites, it is vital improve efficiency of hydrogen evolution reaction (HER). Herein, we constructed Fe-doped molybdenum sulfide encapsulated with N-doped carbon (FeMoSN@NC), which ultrathin edge-curled layer structure. Fe-doping realized Prussian blue nanocages as precursors, plays a crucial role in formation assembled microspheres. Experimental tests theoretical...

A versatile flow cytometric strategy is developed for the sensitive detection of plant microRNA (miRNA) by coupling target-templated click nucleic acid ligation (CNAL) with on-bead terminal enzymatic DNA polymerization (TEP). Unlike ligase-catalyzed reaction, miRNA-templated enzyme-free CNAL between two single-stranded (ssDNA) probes, respectively modified Aza-dibenzocyclooctyne (Aza-DBCO) and N3, can not only simplify operation, but also achieve a much higher efficiency. More importantly,...

Cytosolic Ca2+ ([Ca2+]i) is an important signal that regulates cardiomyocyte differentiation during cardiogenesis. TRPV1 a Ca2+-permeable channel expressed in cardiomyocytes. In the present study, we utilized mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs) as model to investigate functional role of differentiation. Induction cells into was achieved using embryoid body (EB)-based method. Quantitative PCRs showed increased expression process. [Ca2+]i measurement application...

Two Mn(ii) complexes with azide and a new zwitterionic tetracarboxylate ligand 1,2,4,5-tetrakis(4-carboxylatopyridinium-1-methylene)benzene (L(1)), {[Mn5(L(1))2(N3)8(OH)2]·12H2O}n () {[Mn5(L(1))2(N3)8(H2O)2](ClO4)2·6H2O}n (), have been synthesized characterized crystallographically magnetically. contain similar alternating chains constructed by carboxylate bridges. The independent sets of bridges alternate in an ABCCB sequence between adjacent ions: (EO-N3)2 double (EO = end-on) (denoted as...