A5087187918- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Trace Elements in Health
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Selenium in Biological Systems
- Cancer Immunotherapy and Biomarkers
- Transgenic Plants and Applications
- Aging and Gerontology Research
- Insect Resistance and Genetics
- Systemic Sclerosis and Related Diseases
University of Science and Technology of China
2014-2025
Anhui Medical University
2025
Capital Medical University
2024
Ministry of Education
2024
Physical Sciences (United States)
2024
Hefei National Center for Physical Sciences at Nanoscale
2018-2020
The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms. Here, we report the use dual sgRNA-guided to generate knockout mutants protein coding genes, noncoding genes and repetitive sequences C. elegans. Co-injection elegans with sgRNAs results removal interval between two loss-of-function phenotype targeted genes. We sought determine how large an can be eliminated found that at least 24 kb chromosome segment deleted...
Germ granules are biomolecular condensates present in most animal germ cells. One function of is to help maintain cell totipotency by organizing mRNA regulatory machinery, including small RNA-based gene pathways. The C. elegans granule compartmentalized into multiple subcompartments whose biological functions largely unknown. Here, we identify an uncharted subcompartment the granule, which term E granule. nonrandomly positioned within We five proteins that localize RNA-dependent RNA...
Abstract Biomolecular condensates are nonmembrane-enclosed organelles that play important roles in distinct biological processes. Germ granules RNA-rich often docked on the cytoplasmic surface of germline nuclei and exist a variety organisms. The C. elegans germ granule is condensate subcompartmentalized into at least 6 regions, including P granule, Z E Mutator foci, SIMR foci P-body. Although number perinuclear proteins have been found to be enriched granules, their precise localization...
Ribosome biogenesis is a multistep process, during which mistakes can occur at any step of pre-rRNA processing, modification, and ribosome assembly. Misprocessed rRNAs are usually detected degraded by surveillance machineries. Recently, we identified class antisense ribosomal siRNAs (risiRNAs) that down-regulate pre-rRNAs through the nuclear RNAi pathway. To further understand biological roles risiRNAs, conducted both forward reverse genetic screens to search for more suppressor siRNA ( susi...
The nucleolus is the most prominent membraneless organelle within nucleus. How nucleolar structure regulated poorly understood. Here, we identified two types of nucleoli in C. elegans. Type I are spherical and do not have visible vacuoles (NoVs), rRNA transcription processing factors evenly distributed throughout nucleolus. II contain vacuoles, exclusively accumulate periphery rim. NoV contains nucleoplasmic proteins capable exchanging contents with nucleoplasm. high-order dynamically...
Eukaryotic cells express a wide variety of endogenous small regulatory RNAs that function in the nucleus. We previously found erroneous rRNAs induce generation antisense ribosomal siRNAs (risiRNAs) which silence expression via nuclear RNAi defective (Nrde) pathway. To further understand biological roles and mechanisms this class RNAs, we conducted forward genetic screening to identify factors involved risiRNA Caenorhabditis elegans. risiRNAs accumulated RNA exosome mutants. directed...
Histone methylation plays crucial roles in the development, gene regulation, and maintenance of stem cell pluripotency mammals. Recent work shows that histone is associated with aging, yet underlying mechanism remains unclear. In this work, we identified a class putative 3 lysine 9 mono/dimethyltransferase genes (met-2, set-6, set-19, set-20, set-21, set-32, set-33), mutations which induce synergistic lifespan extension long-lived DAF-2 (insulin growth factor 1 [IGF-1] receptor) mutant...
Metazoans utilize the small RNA pathway to regulate gene expression and maintain genome integrity. This directs histone H3 lysine 9 tri-methylation (H3K9me3) or 27 (H3K27me3) at target loci induce transcriptional silencing. Interestingly, some RNAs are generated from genomic enriched in H3K9me3 H3K27me3. However, transcription mechanism of precursors these heterochromatic regions remains unclear. In C. elegans, piRNAs originate two clusters with H3K27me3 marks, which recruit reader UAD-2...
Significance PIWI-interacting RNAs (piRNAs) are highly conserved among metazoan and act to distinguish self nonself nucleic acids, silence transposable elements, defend against viral infections, protect germline integrity, mediate transgenerational inheritance. How piRNA source loci efficiently transcribed is poorly understood. In this work, we used both forward reverse genetic approaches decipher the molecular mechanisms of transcription in Caenorhabditis elegans germline. We identified a...
Temperature greatly affects numerous biological processes in all organisms. How multicellular organisms respond to and are impacted by hypothermic stress remains elusive. Here, we found that cold-warm stimuli induced depletion of the RNA exosome complex nucleoli but enriched it nucleoplasm. To further understand function mechanism stimuli, conducted forward genetic screening identified ZTF-7, which is required for from upon transient exposure C . elegans ZTF-7 a putative ortholog human...
Cadmium ion (Cd2+) is a common environmental pollutant with high biotoxicity. Interestingly, the Cd2+ biotoxicity can be alleviated by coexisting selenite (SeO32–), which induces formation of cadmium selenide-rich nanoparticles (CdSe NPs) under function thiol-capping peptides. However, detailed biochemical mechanisms Cd and Se are synergistically transformed into CdSe NPs in living organisms remain unclear so far. Here, we shed light on molecular basis such biotransformation processes...
Abstract The chromatin organization modifier domain (chromodomain) is an evolutionally conserved motif across eukaryotic species. chromodomain mainly functions as a histone methyl-lysine reader to modulate gene expression, spatial conformation and genome stability. Mutations or aberrant expression of proteins can result in cancer other human diseases. Here, we systematically tag with green fluorescent protein (GFP) using CRISPR/Cas9 technology C. elegans . By combining ChIP-seq analysis...
Abstract The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs (cnRNAs) have been reported to enable precise spatiotemporal control gene expression during division in many species. Whether how cnRNAs exist C. elegans are unclear. Here, using nuclear RNAi Argonaute protein NRDE-3 as reporter, we observed potential peri-centrosome localized small interfering (si)RNAs . was previously shown associate with pre-mRNAs pre-rRNAs via process...
Modification of RNAs, particularly at the terminals, is critical for various essential cell processes; example, uridylation implicated in tumorigenesis, proliferation, stem maintenance, and immune defense against viruses retrotransposons. Ribosomal RNAs can be regulated by antisense ribosomal siRNAs (risiRNAs), which downregulate pre-rRNAs through nuclear RNAi pathway Caenorhabditis elegans. However, biogenesis regulation risiRNAs remain obscure. Previously, we showed that 26S rRNAs are...
Abstract Environmental stimuli not only alter gene expression profiles but also induce structural changes in cells. How distinct nuclear bodies respond to cellular stress is poorly understood. Here, we identified a new subnuclear organelle named the nucleolar body (NoSB), formation of which was induced by inhibition rRNA transcription or inactivation processing and maturation C. elegans . NoSB did colocalize with other previously described organelles. We conducted forward genetic screening...
Environmental stimuli not only alter gene expression profiles but also induce structural changes in cells. How distinct nuclear bodies respond to cellular stress is poorly understood. Here, we identify a subnuclear organelle named the nucleolar body (NoSB), formation of which induced by inhibition rRNA transcription or inactivation processing and maturation C. elegans. NoSB does colocalize with other previously described organelles. We conduct forward genetic screening bZIP factor,...
Pairing center (PC) on each chromosome of Caenorhabditis elegans is crucial for homolog pairing and initiating synapsis. Within PC, clusters 11/12 bp DNA motif recruit one four paralogous meiosis-specific proteins: ZIM-1, ZIM-2, ZIM-3, or HIM-8. However, the mechanistic basis underlying specificity ZIM/HIM-8-PC interaction remains elusive. Here, we describe crystal structures HIM-8, ZIM-1 ZIM-2 binding domains (ZF1, ZF2 CTD) in complex with their cognate PC motifs, respectively. These...
Abstract PIWI-interacting RNAs (piRNAs) are essential for maintaining genome integrity and fertility in various organisms. In flies nematodes, piRNA genes encoded heterochromatinized genomic clusters. The molecular mechanisms of transcription remain intriguing. Through unique indexed-small RNA sequencing chromosome editing, we discovered that spatial aggregation enhances their nematodes. recruits the complex USTC (including PRDE-1, SNPC-4, TOFU-4, TOFU-5) H3K27me3 reader UAD-2, which phase...
Abstract Germ granules are biomolecular condensates present in most animal germ cells. One function of is to help maintain cell totipotency by organizing mRNA regulatory machinery, including small RNA-based gene pathways. The C. elegans granule compartmentalized into multiple subcompartments whose biological functions largely unknown. Here, we identify a new subcompartment the granule, which term E compartment. compartment nonrandomly positioned within granule. We identified five proteins...