A5100392340- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Renal and related cancers
- Zebrafish Biomedical Research Applications
- Genetics and Neurodevelopmental Disorders
- Marine Ecology and Invasive Species
- RNA modifications and cancer
- Tracheal and airway disorders
- Animal Genetics and Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Pharmacological Effects of Natural Compounds
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Plant biochemistry and biosynthesis
- Proteoglycans and glycosaminoglycans research
- Pluripotent Stem Cells Research
- Psidium guajava Extracts and Applications
- Vestibular and auditory disorders
- Environmental DNA in Biodiversity Studies
- Congenital Heart Disease Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hippo pathway signaling and YAP/TAZ
- Marine and coastal ecosystems
- Tissue Engineering and Regenerative Medicine
Shanghai Institute of Technology
2025
New York University
2012-2024
Ocean University of China
2024
National Engineering Research Center of Human Stem Cells
2023
Central South University
2023
Texas Children's Hospital
2017-2020
Baylor College of Medicine
2020
Neurological Research Institute
2017-2020
Wuhan No.1 Hospital
2020
Second Affiliated Hospital of Xi'an Jiaotong University
2019
Abstract Background Glandular trichomes produce a wide variety of commercially important secondary metabolites in many plant species. The most prominent anti-malarial drug artemisinin, sesquiterpene lactone, is produced glandular Artemisia annua . However, only limited genomic information currently available this non-model Results We present global characterization A. trichome transcriptome using 454 pyrosequencing. Sequencing runs two normalized cDNA collections from yielded 406,044...
Cross inhibition between NK4 and TBX1 transcription factors specifies heart versus pharyngeal muscle fates by promoting the activation of tissue-specific regulators in distinct precursors within cardiopharyngeal lineage ascidian, Ciona intestinalis.
The poles of the heart and branchiomeric muscles face neck are formed from cardiopharyngeal mesoderm within pharyngeal apparatus. They disrupted in patients with 22q11.2 deletion syndrome, due to haploinsufficiency TBX1, encoding a T-box transcription factor. Here, using single cell RNA-sequencing, we now identify multilineage primed population mesoderm, marked by Tbx1, which has bipotent properties form cardiac muscle cells. cells localized nascent caudal lateral apparatus provide...
Abstract The composition of the ship's ballast water is complex and contains a large number microalgae cells, bacteria, microplastics, other microparticles. To increase accuracy efficiency detection cells in water, new microfluidic chip for continuous separation based on alternating current dielectrophoresis was proposed. In this chip, one piece 3‐dimensional electrode embedded side eight discrete electrodes are arranged microchannel. An insulated triangular structure between designed...
Integrated analyses of regulated effector genes, cellular processes, and extrinsic signals are required to understand how transcriptional networks coordinate fate specification cell behavior during embryogenesis. Ciona cardiopharyngeal progenitors, the trunk ventral cells (TVCs), polarize as leader trailer that migrate between epidermis endoderm. We show TVC-specific collagen-binding Discoidin-domain receptor (Ddr) cooperates with Integrin-β1 promote cell-matrix adhesion. find endodermal...
Neurogenesis is a complex sequence of cellular processes and behaviors driven by the coordinated expression conserved effectors. The bipolar tail neurons (BTNs) Ciona develop according to highly dynamic, yet stereotyped developmental program thus could serve as an accessible model system for neurogenesis, including underlying cell like neuronal delamination, migration, polarized axon outgrowth. Here we investigate both upstream events that shape BTN neurogenesis through spatiotemporal...
Considerable difference in artemisinin and its direct precursors, artemisinic acid dihydroartemisinic acid, was detected between two chemotypes within the species Artemisia annua (A. annua). These showed differential metabolic response to methyl jasmonate (MeJA) elicitation. Exogenous application of MeJA resulted an accumulation Type I plants. In II plants, however, level decreased dramatically under Squalene other sesquiterpenes, (e.g., caryophyllene, germacrene D), were stimulated by both...
Fast on-site monitoring of foreign microalgae species carried by ship ballast water has drawn more and attention. In this paper, we presented a new method compact device classification cells simultaneous detection three kinds signals single in disposable microfluidic chip. The advantages fast detection, low cost, portability. cell can be identified chlorophyll fluorescence (CF), side light scattering (SLS), resistance pulse sensing (RPS) the cell. These represent different characteristics A...
Abstract In vertebrates, multipotent progenitors located in the pharyngeal mesoderm form cardiomyocytes and branchiomeric head muscles, but dynamic gene expression programs mechanisms underlying cardiopharyngeal multipotency heart vs. muscle fate choices remain elusive. Here, we used single cell genomics simple chordate model Ciona, to reconstruct developmental trajectories forming first second lineages, precursors, characterize molecular underpinnings of choices. We show that FGF-MAPK...
A large number of genes have been implicated in neurodevelopmental disorders (NDDs), but their contributions to NDD pathology are difficult decipher without understanding diverse roles different brain cell types. Here, we integrated genetics with single-cell RNA sequencing data assess coexpression enrichment patterns various gene sets. We identified midfetal cortical neural progenitor development—more specifically, the ventricular radial glia-to-intermediate transition at gestational week...
Purpose: This study aimed to assess the clinical value of ocular vestibular evoked myogenic potential (oVEMP) and cervical (cVEMP) for monitoring rehabilitation function in patients treated peripheral vertigo.Materials methods: Fifteen who had been diagnosed with vertigo showed no VEMP response on affected side but exhibited symptom alleviation responses after therapies were retrospectively enrolled. We analyzed restoration parameters their response.Results: After treatment, six sudden...
Abstract The tadpole-type larva of Ciona has emerged as an intriguing model system for the study neurodevelopment. intestinalis connectome been recently mapped, revealing smallest central nervous (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent larger vertebrates, sharing many conserved developmental processes, anatomical compartments, neuron subtypes, and even specific neural circuits. Thus, tadpole offers a unique opportunity to understand...
Abstract During development, stem and progenitor cells divide transition through germ layer- lineage-specific multipotent states to generate the diverse cell types that compose an animal. Defined changes in biomolecular composition underlie progressive loss of potency acquisition characteristics. For example, cardiopharyngeal progenitors display multilineage transcriptional priming, whereby both cardiac pharyngeal muscle programs are partially active coexist same cells, while their daughter...