A5100388521- MicroRNA in disease regulation
- Food composition and properties
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Polysaccharides Composition and Applications
- Retinoids in leukemia and cellular processes
- Microbial Metabolites in Food Biotechnology
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- RNA Research and Splicing
- Antioxidant Activity and Oxidative Stress
- Renal Diseases and Glomerulopathies
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Proteins in Food Systems
- Phytase and its Applications
- Food Quality and Safety Studies
- RNA Interference and Gene Delivery
- Pregnancy and preeclampsia studies
- Inflammation biomarkers and pathways
- RNA modifications and cancer
- Cytokine Signaling Pathways and Interactions
- Freezing and Crystallization Processes
- CAR-T cell therapy research
Southern University of Science and Technology
2021-2025
Henan University of Technology
2011-2024
Harbin Medical University
2005-2024
First Affiliated Hospital of Harbin Medical University
2005-2024
China Pharmaceutical University
2015-2024
Changsha Normal University
2024
Hunan Normal University
2024
Zhongshan People's Hospital
2024
Nanjing Drum Tower Hospital
2024
Tianjin Medical University General Hospital
2024
Diagnosis of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), particularly HCC independent cirrhosis etiology, presents a great challenge because lack biomarkers. Here we test the hypothesis that expression profiles microRNAs (miRNAs) in serum can serve as biomarkers for diagnosis HBV infection and HBV-positive HCC. We recruited 513 subjects (210 controls 135 HBV-, 48 C (HCV)-, 120 HCC-affected individuals) employed strategy initial screening by Solexa sequencing followed...
BACKGROUND Although microRNAs (miRNAs) play essential roles in spermatogenesis, little is known about seminal plasma miRNAs infertile men. We investigated the profile of men to identify that are altered infertility; we then evaluated their diagnostic value. METHODS Seminal samples were obtained from 289 and 168 age-matched fertile control individuals. The stability was first assessed by time-course freeze–thaw cycle analyses. Solexa sequencing technology used for an initial screen pooled 45...
An increased population of CD4+CD25highFoxp3+ regulatory T cells (Tregs) in the tumor-associated microenvironment plays an important role cancer immune evasion. However, underlying mechanism remains unclear. Here we observed secretion miR-214 various types human cancers and mouse tumor models. Tumor-secreted was sufficiently delivered into recipient by microvesicles (MVs). In targeted peripheral CD4+ cells, tumor-derived efficiently downregulated phosphatase tensin homolog (PTEN) promoted...
Platelets play a significant role in atherosclerosis, stroke, and asthma through active interaction with neutrophils, monocytes, vascular endothelial cells. The mechanism underlying these intercellular interactions, however, is incompletely understood. In this study, we report that platelets can remotely modulate cell apoptosis releasing microRNA-223 (miR-223)-containing microvesicles (MVs). First, expressed abundant miRNAs, miR-223 had the highest level of expression. Platelet other miRNAs...
Anti-inflammatory polarized macrophages are reported to alleviate systemic lupus erythematosus (SLE). Our previous studies have demonstrated that exosomes from adipose-derived stem cells promote the anti-inflammatory polarization of macrophages. However, possible therapeutic effect on SLE remains unexplored.Exosomes were isolated conditioned medium bone marrow-derived mesenchymal using ultrafiltration and size-exclusion chromatography identified by nanoparticle tracking analysis...
Cell-secreted miRNAs are highly stable and can serve as biomarkers for various diseases signaling molecules in intercellular communication. The mechanism underlying the stability of circulating miRNAs, however, remains incompletely understood. Here we show that Argonaute 2 (Ago2) complexes microvesicles (MVs) provide specific non-specific protection miRNA cell-secreted MVs, respectively. First, resistance MV-encapsulated to RNaseA was both depended on intact vesicular structure MVs...
The involvement of circulating microRNAs (miRNAs) in cancer and their potential as biomarkers diagnosis prognosis are becoming increasingly appreciated; however, little is known about miRNA profiles astrocytomas. In our study, we performed genome-wide serum analysis by the Solexa sequencing followed validation conducted training verification sets with a stem-loop quantitative reverse-transcription PCR (RT-qPCR) assay from samples 122 untreated astrocytomas patients (WHO grades III-IV) 123...
Patients with hematogenous metastatic lung cancer displayed significantly increased platelet count and aggregation compared to patients without metastasis. The mechanism underlying the correlation between metastasis activation remains unknown. In present study, we explored role of microRNA-223 (miR-223) derived from platelets in modulating cell invasion. Our results demonstrated that NSCLC contain higher level miR-223 than healthy subjects. concentration platelet-secreted microvesicles...
Shift metabolism profile from mitochondrial oxidative phosphorylation to aerobic glycolysis (Warburg effect) is a key for tumor cell growth and metastasis. Therefore, suppressing the shows great promise in anti-tumor therapy. In present study, we study role of shikonin, naphthoquinone isolated traditional Chinese medicine Lithospermum, inhibiting thus growth. We found that shikonin dose-dependently inhibited glucose uptake lactate production Lewis lung carcinoma (LLC) B16 melanoma cells,...
Chronic inflammatory bowel diseases (IBDs) are associated with differential expression of genes involved in inflammation and tissue remodelling. We surveyed the profile apoptosis-related microRNAs by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) a dextran sulphate sodium (DSS) murine model colitis. found that miR-150 was strongly elevated, whereas c-Myb, transcription factor target gene miR-150, significantly reduced colon after DSS treatment....
Shikonin is a naphthoquinone isolated from the traditional Chinese medicine Lithospermum. It has been used in treatment of various tumors. However, effects shikonin on such diseases have not fully elucidated. In present study, we detected exosome release breast cancer cell line (MCF-7) with and found positive relationship between level secreted exosomes proliferation. We next analyzed miRNA profiles MCF-7 cells that some miRNAs are specifically sorted abundant exosomes. Knockdown most...
Abstract Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning diameter range 500 to 3000 nm. They are emitted by migrating cells and harbour diverse array RNAs proteins. can be readily identified in bodily fluids like serum urine, rendering them valuable non‐invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce streamlined effective approach the capture quantitative assessment migrasomes, employing wheat germ...
This study aimed to identify urinary microRNAs (miRNAs) as biomarkers for FSGS disease activity.Candidate miRNAs were identified in pooled urine samples from patients with active (FSGS-A) and remission (FSGS-CR), then validated using individual samples. Their levels compared both under different treatment responses a prospective of membranous nephropathy (MN) diabetic (DN) activity. The prediction these was further analyzed retrospective cohorts FSGS.All 54 included candidate biomarkers,...
Abstract Rapid corrosion of magnesium alloys is undesirable in structural and biomedical applications a general way to control form surface barrier layer isolating the bulk materials from external environment. Herein, based on insights gained anticorrosion behavior products, special mitigate aqueous described. The concept pre-corrosion by hydrothermal treatment Al-enriched Mg water. A uniform composed an inner compact top Mg-Al layered double hydroxide (LDH) microsheet produced large area...
Hepatic miR-122 can serve as a pro-apoptotic factor to suppress tumorigenesis. The underlying mechanism, however, remains incompletely understood. Here we present the first evidence that promotes hepatocellular carcinoma cell apoptosis through directly silencing biogenesis of survival oncomiR miR-21 at posttranscriptional level. We find is strongly expressed in primary liver nucleus but its nuclear localization markedly decreased transformed cells particularly chemoresistant tumor cells....
Infection of H5N1 influenza virus causes the highest mortality among all viruses. The mechanisms underlying such high viral pathogenicity are incompletely understood. Here, we report that encodes a microRNA-like small RNA, miR-HA-3p, which is processed from stem loop-containing RNA precursor by Argonaute 2, and plays role in enhancing cytokine production during infection. Mechanistic study shows miR-HA-3p targets poly(rC)-binding protein 2 (PCBP2) suppresses its expression. Consistent with...
Organ-specific microRNAs have essential roles in maintaining normal organ function. However, the microRNA profile of kidney and role modulating renal function remain undefined. We performed an unbiased assessment genome-wide expression 14 mouse organs using Solexa deep sequencing found that microRNA-196a (miR-196a) miR-196b are selectively expressed kidney, with 74.37% total miR-196a 73.19% distributed kidneys. confirmed predominant miR-196a/b human particularly glomeruli tubular epithelium,...