A5100623265- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Erythrocyte Function and Pathophysiology
- Genomics and Phylogenetic Studies
- Hemoglobin structure and function
- Adipose Tissue and Metabolism
- Circular RNAs in diseases
- Blood properties and coagulation
- Genetic diversity and population structure
- Identification and Quantification in Food
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Natural product bioactivities and synthesis
- Heat shock proteins research
- Cholinesterase and Neurodegenerative Diseases
- Vagus Nerve Stimulation Research
- Colorectal Cancer Surgical Treatments
- Characterization and Applications of Magnetic Nanoparticles
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Adhesion Molecules Research
- Biochemical and Structural Characterization
Taian City Central Hospital
2024
Qingdao University
2024
Nanjing University
2011-2023
Beijing Academy of Agricultural and Forestry Sciences
2023
Poultry Research Institute
2023
State Key Laboratory of Pharmaceutical Biotechnology
2014-2023
Nanjing Tech University
2023
Yulin Normal University
2023
Chinese Academy of Sciences
2020
Agricultural Genomics Institute at Shenzhen
2020
Platelets play a significant role in atherosclerosis, stroke, and asthma through active interaction with neutrophils, monocytes, vascular endothelial cells. The mechanism underlying these intercellular interactions, however, is incompletely understood. In this study, we report that platelets can remotely modulate cell apoptosis releasing microRNA-223 (miR-223)-containing microvesicles (MVs). First, expressed abundant miRNAs, miR-223 had the highest level of expression. Platelet other miRNAs...
Alzheimer’s disease (AD) is the most common type of dementia, and promptly diagnosis AD crucial for delaying development improving patient quality life. However, detection, particularly in early stages, remains a substantial challenge due to lack specific biomarkers. The present study was undertaken identify validate potential circulating miRNAs as novel biomarkers AD. Solexa sequencing employed screen expression profile serum controls. RT-qPCR used confirm altered at individual level....
The steadily increasing incidence of kidney injury is a significant threat to human health. current tools available for the early detection injury, however, have limited sensitivity or specificity. Thus, development novel biomarkers detect high importance. Employing mouse renal ischemia-reperfusion and streptozotocin (STZ)-induced as acute chronic model, respectively, we assessed alteration microRNA (miRNA) in urine, serum tissue by TaqMan probe-based qRT-PCR assay. Our results demonstrated...
The ATP-dependent Clp protease (ClpP) plays an essential role not only in the control of protein quality but also regulation bacterial pathogen virulence, making it attractive target for antibacterial treatment. We have previously determined crystal structures Staphylococcus aureus ClpP (SaClpP) two different states, extended and compressed. To investigate dynamic switching between these we performed a series molecular dynamics simulations. During structural transition, long straight helix E...
Cavities are important in clinical diagnosis of pulmonary tuberculosis (TB) infected by Mycobacterium tuberculosis. Although microRNAs (miRNAs) play a vital role the regulation inflammation, relation between plasma miRNA and with cavity remains unknown. In this study, samples were derived from 89 cavitary (CP-TB) patients, non-cavitary (NCP-TB) patients 95 healthy controls. Groups matched for age gender. screening phase, Illumina high-throughput sequencing technology was employed to analyze...
Background. The mechanism underlying the ability of virulent Salmonella organisms to escape clearance by macrophages is incompletely understood. Here, we report a novel which escapes macrophages. Methods. Microarray and quantitative real-time polymerase chain reaction analyses were used screen key microRNAs regulating Salmonella–host cell interactions. Target gene was tested using luciferase reporter Western blot assays. role microRNA 128 (miR-128) assayed intestinal epithelial cells mouse...
Lysine-specific demethylase 1 (LSD1), the first identified histone demethylase, is a flavin-dependent amine oxidase which specifically demethylates mono- or dimethylated H3K4 and H3K9 via redox process. It participates in broad spectrum of biological processes high importance cell proliferation, adipogenesis, spermatogenesis, chromosome segregation embryonic development. To date, as potential drug target for discovering anti-tumor drugs, medical significance LSD1 has been greatly...
The present study aimed to investigate the functional components from Bushen Yijing Formula and their inhibition of endothelial-mesenchymal transition (EndMT) fibrosis in human umbilical vascular endothelial cells (HUVECs). HUVEC was induced by treatment transforming growth factor β (TGF-β) as cellular model. Expression EndMT biomarker gene cofactors were determined quantitative real-time-PCR, western blotting, immunofluorescence. Angiogenesis capacity vein evaluated using tube formation...
Avibacterium paragallinarum is the pathogen that causes infectious coryza, a highly contagious respiratory disease brings serious threat to chickens. Heme utilization systems play an important role in bacterial adversity adaptation and pathogenicity, our previous report found presence of heme (HutZ) Av. paragallinarum. However, little known about function HutZ In this study, mutant strain was successfully developed identified by PCR western blot analysis. Mutation significantly retards...
The complete mitochondrial genome sequence of Great tit Parus major was sequenced used polymerase chain reaction (PCR), long-and-accurate PCR and directly sequencing by primer walking. Genbank accession KP137624. entire P. is a circular molecule 16,776 bp in length the content A, T, C G were 29.68%, 22.63%, 33.56% 14.13%, respectively. contains 13 protein-coding genes, 2 rRNA 22 tRNA plus 1 control regions similar to most other Aves birds gene arrangement composition. could provide useful...
Abstract Background Cyperus rotundus L. is a notorious weed that harms agricultural ecosystems worldwide. Although the chloroplast genome (cpgenome) of C. has been studied, there have no reports on mitochondrial (mitogenome) . Results The mitogenome and cpgenome XFZ01 four circular DNA moleculesand one molecule, respectively. Their total lengths were 1,491,358 bp 186,119 bp, contained 75 genes, including 40 protein-coding genes (PCGs), 9 rRNA 26 tRNA genes. 121 69 PCGs, 8 44 Analysis...
Uncoupling protein 1 (UCP1) located at the mitochondrial inner membrane serves an important role in adaptive non-shivering thermogenesis. Previous data has demonstrated that lipids regulate biological functions of proteins. However, how interact with UCP1 still remains elusive. In this study, interactions between and were investigated, using bioinformatic approaches due to limitations associated experimental techniques. A total 8 peptide regions α-helices identified related functional sites...
Magnetic levitation (MagLev) is a promising density-based analytical technique with numerous applications. Several MagLev structures different levels of sensitivity and range have been studied. However, these can seldom satisfy the performance requirements simultaneously, such as high sensitivity, wide measurement range, easy operation, which prevented them from being widely used. In this work, tunable system was developed. It confirmed by numerical simulation experiments that possesses...
This study aimed to investigate the correlation between baseline MRI features and carcinoembryonic antigen (CEA) expression status in rectal cancer patients. A training cohort of 168 patients from Center 1 an external validation 75 2 were collected. nomogram was constructed based on validated using predict high CEA The nomogram's discriminative ability clinical utility tested receiver operating characteristic (ROC) curve, decision curve analysis (DCA). high-expression group had significantly...
In this study, the complete mitochondrial genome of T. kessleri was sequenced and characterized. The overall base composition mitogenome is 29.3% for A, 32.3% C, 15.0% G, 23.5% percentage G + C content 47.2%. a circular DNA molecule 16,754 bp in length, including D-loop region, two rRNA genes (12SrRNA 16SrRNA), 13 protein-coding (PCGs), 22 tRNA genes. sequence information can contribute to enrich molecular data resources about birds could also enable phylogenetic research help resolve...
Abstract Human cytomegalovirus (HCMV), infects a majority of population and can undergo life-long, latent infection, is leading cause opportunistic congenital disease. HCMV encode 17 microRNAs (miRNAs) but the roles these viral miRNAs during infection are unclear. Here, we infected human bone marrow CD34+ hematopoietic progenitor Kasumi-3 cells with (strain NR-1) determined kinetics cellular levels all hcmv by qRT-PCR. We found that, different from most other which had high level at early...
Abstract Wild castor grows in the high-altitude tropical desert of African Plateau, a region that has high ultraviolet radiation, strong light and extremely dry conditions. Some genes or alleles persist wild genome to prevent species being vanished at extreme condition, but usually be decayed under domestication due relaxation natural selection. To investigate potential genetic basis adaptation both highland deserts, we generated chromosome-level sequence accession WT05 with scaffold contig...