Objective Escherichia coli ( E. ) is the most common opportunistic clinical micro-organism with high drug resistance. This study aimed to analyse resistance pattern of according patient age and sample type. Design setting retrospective observational was conducted in a tertiary hospital southeastern China. Participants strains were isolated from blood, urine sputum infected inpatients. The patients divided into four groups: children (0–14 years old, including neonatal non-neonatal groups),...

Cavities are important in clinical diagnosis of pulmonary tuberculosis (TB) infected by Mycobacterium tuberculosis. Although microRNAs (miRNAs) play a vital role the regulation inflammation, relation between plasma miRNA and with cavity remains unknown. In this study, samples were derived from 89 cavitary (CP-TB) patients, non-cavitary (NCP-TB) patients 95 healthy controls. Groups matched for age gender. screening phase, Illumina high-throughput sequencing technology was employed to analyze...

LettersMarch 2023Second Identified Human Infection With the Avian Influenza Virus H10N3: A Case ReportFREEWeimin Zhang, MD, Zhongheng PhD, Maofeng Wang, Xinling Pan, Xuandong Jiang, MDWeimin MDIntensive Care Unit, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, China, PhDDepartment Emergency Medicine, Sir Run Zongyang Shaw Hospital, Zhejiang University School Hangzhou, Biomedical Sciences Laboratory, ChinaAuthor, Article, and Disclosure...

Background B lymphocytes play a key role in immunosuppression. This study investigated the prognostic value of cell subsets sepsis. Methods Flow cytometry was used to assess peripheral from patients with sepsis on first and seventh days following admission, as well 111 healthy controls. The were divided into survivors non-survivors, based 28-day prognosis. Results analysis showed abnormal distribution selective depletion cells its early stages On day 1, compared survivors, non-survivors...

MPT64 protein is an effective marker for detecting Mycobacterium tuberculosis (MTB) in liquid culture and clinical tissue samples. However, some MTB isolates test negative this antigen because of varied mutation types across different regions. DNA samples assay-negative strains were collected from a tertiary hospital January 2016 to 2024, mpt64 gene mutations detected by sequencing. Clinical records patients with results compared those positive results. The global distribution was analyzed...

Background A prediction model is hereby developed to identify poor treatment outcomes during the intensive phase in patients with initial of pulmonary tuberculosis (TB). Methods The data inpatients TB were collected from a tertiary hospital located Southeastern China July 2019 December 2023. included divided into modeling group and validation group. outcome indicator was based on comparison CT findings before after two-month anti-TB treatment. In group, independent risk factors obtained...

Mycobacterium seoulense (M. seoulense) is an emerging pathogen increasingly associated with infections; however, its resistance phenotypes and genomic characteristics remain largely unknown. Seven M. isolates were collected from clinical samples. Drug susceptibility testing was conducted using Sensititre™ SLOMYCO2 plates. Whole genome sequencing supporting bioinformatics analyses performed to analyze the features. All (n=7) exhibited growth on 7H10 agar medium containing thiophenecarboxylic...

Difficult-to-treat resistant Pseudomonas aeruginosa (DTR-PA) is an MDR subset to all first-line antipseudomonal agents. It particularly concerning in respiratory infections like bronchiectasis, leading poor outcomes, limited treatment options, and higher healthcare costs. This study aimed investigate the antimicrobial resistance profiles sequence types (STs) of isolates, providing insight into their patterns factors contributing resistance. In 2021, 38 multidrug-resistant P. isolates were...

Objectives: To establish an individualized nomogram to predict the probability of drug-induced liver injury (DILI) in tuberculosis patients receiving anti-tuberculosis treatment. Methods: The clinical information admitted a tertiary hospital between January 2010 and December 2022 was retrospectively reviewed from records. Patients with baseline diseases (hepatis B or C infection fatty liver) taking protective drugs were excluded. maximum values function test within 180 days after treatment...

Objective To establish and validate an individualized nomogram to predict mortality risk within 30 days in patients with sepsis from the emergency department. Methods Data of 1205 who were admitted department a tertiary hospital between Jun 2013 Sep 2021 collected divided into training group validation at ratio 7:3. The independent factors related 30-day identified by univariate multivariate analysis used construct nomogram. model was evaluated receiver operating characteristic (ROC) curve,...

Background Urinary tract infections (UTI) is a prevalent condition in those with diabetes, and severe cases, it may escalate to sepsis. Therefore, important analyze the risk variables associated sepsis diabetes individuals UTI. Methods This research was retrospective cross-sectional analysis. From January 2011 June 2022, group of were identified as having UTI at tertiary hospital situated Southeastern China. Patient data, including information on urine culture, collected retrospectively from...

The increased prevalence of Klebsiella pneumoniae infections and resistance rates are a current cause for concern. However, data in K. strains from primary hospitals the distribution among different isolate sample sources scarce.All were isolated patients who visited health care center located Central Zhejiang Province January 2011 to December 2017. specimens included blood, sputum, cervical secretions urine. species identified by Vitek 2 Compact Bacterial Identification Monitoring System or...

The spread of multidrug-resistant tuberculosis (MDR-TB) Mycobacterium (M. tuberculosis) strains has been a big challenge to the TB control and prevention in China. Knowledge about patterns drug resistance high-burden areas China is crucial develop appropriate strategies. We conducted comprehensive investigation pattern M. Heilongjiang Province.1427 clinical were isolated from pulmonary patients hospitalized between 2007 2012. susceptibility isolates first-line anti-TB drugs MDR...

Abstract To achieve a comprehensive understanding of the characteristics patients with non-tuberculous mycobacteria (NTM), NTM between January 2016 and June 2019 were recruited from primary hospital. identified based on MBP64 protein assay. The clinical records laboratory assay results retrospectively reviewed. A total 204 included in final analysis. multiple isolations more likely accompanied chronic obstructive pulmonary disease (COPD) (p = 0.029) arthritis 0.049), but showed lower...

We attempted to establish a model for predicting the mortality risk of sepsis patients during hospitalization.Data on with were collected from clinical record mining database, who hospitalized at Affiliated Dongyang Hospital Wenzhou Medical University between January 2013 and August 2022. These included divided into modeling validation groups. In group, independent factors death hospitalization determined using univariate multi-variate regression analyses. After stepwise analysis (both...

Rheumatoid arthritis (RA) and osteoarthritis (OA) share some similar arthritic symptoms, but different mechanisms underlie the pathogenesis of these two diseases. Analysis differentially expressed molecules in rheumatoid may assist improving diagnosis treatment strategies clinical practice.Microarray RNA-seq data were acquired from gene expression omnibus database. Differentially genes (DEGs) identified using Bioconductor packages. Receiver operating characteristic curves plotted to assess...

Acute cholangitis is a clinical syndrome caused by bacterial infection in the biliary system. The bacteria could exist bile before drainage despite empirical antibiotic treatment.

Herein, we report a case of lymphadenitis caused by Mycobacterium chimaera . A 54-year-old woman with chronic myeloproliferative neoplasm was admitted to the hospital cervical lymphadenopathy. After preliminary exclusion various diseases such as lymphoma, Epstein–Barr virus infection, and autoimmune disease, lymph node biopsy specimen showed epithelioid granulomatous caseous necrosis, epithelial-like cells, multinucleated giant cells seen in tuberculosis (TB). Although never isolated,...

Although several optimal mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) loci have been suggested for genotyping homogenous Mycobacterium tuberculosis, including the Beijing genotype, a more efficient and convenient selection strategy identifying VNTR is needed. Here 281 M. tuberculosis isolates were analyzed. genotype non-Beijing genotypes identified, as well sublineages, according to single nucleotide polymorphisms. A total of 22 MIRU-VNTR used...

Introduction: Non-tuberculous mycobacterium (NTM) can colonize the human body, leading to opportunistic infection. This study was conducted analyze NTM species composition in a primary hospital and investigate potential features of patients with different species.
 Methodology: Mycobacterial strains were collected from admitted at January 2016 May 2019. MPB64 assay used screen confirmed by Rv0577 amplification. The identified hsp65 sequencing. clinical records retrospectively...

Abstract Background Escherichia hermannii (E. hermanni) is always accompanied by other bacterial infections in humans. In previous reports, most E. -related were caused sensitive strains. Here, for the first time, we report case of a patient with New Delhi metallo-β-lactamase (NDM)-positive bloodstream infection. Case presentation The was 70-year-old male admitted to our hospital due 4-day fever, history malignant tumor, liver cirrhosis, and chronic obstructive pulmonary disease. After...

Objective Behavioral intentions to care for patients with infectious diseases are crucial improving quality of care. However, there have been few studies the behavioral and factors influencing patient by clinical nurses during COVID-19 pandemic. This study aims explore cognition, attitudes, subjective norms, self-efficacy, while caring any factors. Method A cross-sectional survey was conducted through convenience sampling in southeast China from February 2020 March 2020. The questionnaire...

We intend to develop a nomogram for predicting the mortality risk of hospitalized septic shock patients.Data were collected from patients with in Affiliated Dongyang Hospital Wenzhou Medical University China, over 10 years between January 2013 and 2023. The eligible study participants divided into modeling validation groups. Factors independently related group obtained by stepwise regression analysis. A logistic model built. was evaluated based on discrimination power (the area under curve...