A5005324730- Chromatin Remodeling and Cancer
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Cancer-related gene regulation
- Mechanisms of cancer metastasis
- Cancer-related molecular mechanisms research
- Cardiac Fibrosis and Remodeling
- Sirtuins and Resveratrol in Medicine
- Liver physiology and pathology
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- interferon and immune responses
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Peroxisome Proliferator-Activated Receptors
- Immune cells in cancer
- Endoplasmic Reticulum Stress and Disease
- NF-κB Signaling Pathways
- Atherosclerosis and Cardiovascular Diseases
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
Chinese PLA General Hospital
2025
Guangxi Medical University
2025
Nanjing Medical University
2015-2024
Liaocheng University
2018-2024
Xian Yang Central Hospital
2024
China Pharmaceutical University
2015-2024
Third Xiangya Hospital
2019-2024
Central South University
2019-2024
Target (United States)
2018-2023
Shenzhen Second People's Hospital
2023
Tumor necrosis factor-alpha (TNF) and interleukin-1beta (IL-1) are cytokines that induce expression of various genes through activation the redox-sensitive transcription factor nuclear factor-kappaB (NF-kappaB). We have previously cloned entire human MnSOD (SOD2) gene found several NF-kappaB-binding sites in 5' 3' flanking intronic regions. To test whether these putative able to respond TNF IL-1, we performed induction analysis using deletion constructs ligated a luciferase reporter gene....
Results Allergic SLPI transgenic mice showed a significant decrease in airway resistance compared to wild-type (6.3 ± 1.1 vs. 8.0 2.1 cm H20 × s/ml, p < 0.001), the same effect was observed with inflammatory cell infiltration, eosinophil percentage (24 1.1% 29 2.3%, goblet cells (6 1.4 36 4.0%, 0.001) lungs and IgE levels (2014.1 309.2 4173.2 685.6 ng/ml, plasma. knock-out displayed significantly higher values mice. They include lung (8.6 2.7 6.6 0.5 H20*s/ml, influx, eosinophils (36.0 29.0...
Diabetic nephropathy (DN) is one of the most common complications associated with diabetes and characterized by renal microvascular injury along accelerated synthesis extracellular matrix proteins causing tubulointerstitial fibrosis. Production type I collagen, major component matrix, augmented during fibrosis after chronic exposure to hyperglycemia. However, transcriptional modulator responsible for epigenetic manipulation leading induction collagen genes not clearly defined. We show here...
Abstract Macrophage-orchestrated, low-grade chronic inflammation plays a pivotal role in obesity and atherogenesis. However, the underlying regulatory mechanisms remain incompletely understood. Here, we identify major vault protein (MVP), main component of unique cellular ribonucleoprotein particles, as suppressor for NF-κB signaling macrophages. Both global myeloid-specific MVP gene knockout aggravates high-fat diet induced obesity, insulin resistance, hepatic steatosis atherosclerosis...
Epithelial-to-mesenchymal transition (EMT) plays a pivotal role in lung cancer metastasis. The class III deacetylase sirtuin 1 (SIRT1) possesses both pro- and anticarcinogenic properties. of SIRT1 EMT is largely undefined.The effect on migration cells was evaluated by wound healing assay vitro metastasis nude mice vivo. Protein expression human cancers cultured assessed western blotting immunohistochemistry. Interaction between protein DNA measured chromatin immunoprecipitation assay....
Macrophage-mediated inflammatory response represents a key pathophysiological process in host of cardiovascular diseases including heart failure. Regardless aetiology, failure is invariably preceded by cardiac hypertrophy. In the present study, we investigated effect macrophage-specific deletion myocardin-related transcription factor A (MRTF-A) on hypertrophy and underlying mechanism.We report that when subjected to transverse aortic constriction (TAC), macrophage MRTF-A conditional knockout...
Abstract Alcoholic liver disease (ALD) accounts for a large fraction of patients with cirrhosis and hepatocellular carcinoma. In the present study we investigated involvement Brahma‐related gene 1 (Brg1) in ALD pathogenesis implication intervention. We report that Brg1 expression was elevated mouse models ALD, hepatocyte exposed to alcohol, human specimens. Manipulation hepatocytes influenced development mice. Flow cytometry showed deficiency specifically attenuated hepatic infiltration Ly6G...
Objective Liver fibrosis is a prelude to host of end-stage liver diseases. Hepatic stellate cells (HSCs), switching from quiescent state myofibroblasts, are the major source for excessive production extracellular matrix proteins. In present study, we investigated role Suv39h1, lysine methyltransferase, in HSC-myofibroblast transition and implication fibrosis. Design HSC-specific or myofibroblast-specific Suv39h1 deletion was achieved by crossbreeding f/f mice Lrat -Cre Postn ERT2 mice....
Zero-shot visual question answering (ZS-VQA), an emerged critical research area, intends to answer questions without providing training samples. Existing in ZS-VQA has proposed leverage knowledge graphs or large language models (LLMs), respectively, as external information sources help VQA model comprehend images and questions. However, LLMs often struggle accurately interpreting specific meanings. Meanwhile, although graph rich entity relationships, it is challenging effectively connect...
Survivin, a unique antiapoptotic factor, plays an important role in cell cycle regulation. Numerous clinical studies have shown that survivin is markedly overexpressed most common types of cancer, suggesting transcriptional deregulation major mechanism involved aberrant expression cancers. In this study, we identified several polymorphisms the gene promoter. One these located at CDE/CHR repressor elements, and appears to be mutation with high frequency among cancer lines compared normal line...
Atherosclerosis proceeds through a multistep reaction that begins with endothelial injury caused by host of stress signals, among which oxidized low-density lipoprotein (oxLDL) plays critical role. OxLDL disrupts normal functionality the endothelium upregulating adhesion molecules (eg, ICAM-1) and concomitantly downregulating nitric oxide synthase (eNOS) expression. The transcriptional modulator mediates cellular response to oxLDL remains largely obscure.Our goal was determine whether...
Chronic inflammation, inflicted by the spillover of proinflammatory mediators, links metabolic dysfunction to nonalcoholic steatohepatitis (NASH). The epigenetic maneuverings that underscore accelerated synthesis mediators in response nutritional inputs are not clearly defined. Here we report ATP-dependent chromatin remodeling proteins Brahma-related gene 1 (Brg1) and Brahma (Brm) were up-regulated vitro cultured hepatocytes treated with free fatty acid or glucose vivo animal models NASH....
Rationale: Endothelial dysfunction inflicted by inflammation is found in a host of cardiovascular pathologies. One hallmark event this process the aggregation and adhesion leukocyte to vessel wall mediated upregulation molecules (CAM) endothelial cells at transcriptional level. The epigenetic modulator(s) CAM transactivation its underlying pathophysiological relevance remain poorly defined. Objective: Our goal was determine involvement Brahma related gene 1 (Brg1) (Brm) pathogenesis...
Epithelial-mesenchymal transition (EMT) plays an essential role in organogenesis and contributes to a host of pathologies including carcinogenesis. Hypoxia aids tumor metastasis part by promoting EMT cancer cells. The underlying mechanism whereby hypoxia orchestrates remains poorly defined. Here we report that SIRT1, multifaceted player tumorigenesis, opposed ovarian vitro vivo impeding EMT. Hypoxic stress down-regulated SIRT1 expression primarily at the transcriptional level reducing...
Chronic inflammation underscores the pathogenesis of a range human diseases. Lipopolysaccharide (LPS) elicits strong pro-inflammatory response in macrophages via transcription factor NF-κB. The epigenetic mechanism underlying LPS-induced is not completely appreciated. Herein we describe role for myocardin related A, or MRTF-A, this process. MRTF-A over-expression potentiated while silencing dampened NF-κB dependent transcription. deficiency also alleviated synthesis mediators mouse model...
Activation of hepatic stellate cells (HSC) is a hallmark event in liver fibrosis. Accumulation reactive oxygen species (ROS) serves as driving force for HSC activation. The regulatory subunits the NOX complex, NCF1 (p47phox) and NCF2 (p67phox), are up-regulated during activation contributing to ROS production transcriptional mechanism underlying NCF1/2 up-regulation not clear. In present study we investigated role serum response factor (SRF) focusing on regulation NCF1/2. We report that...
ABSTRACT Liver regeneration is a complicated pathophysiologic process that regulated by myriad of signaling pathways and transcription factors. The interaction among these factors, either cooperatively or antagonistically, may ultimately lead to recovery restoration liver function permanent loss failure. In the present study, we investigated mechanism whereby chromatin remodeling protein brahma related gene 1 (Brg1) regulates in mice. Smarca4 ‐Flox strain mice was crossbred with Alb‐ Cre...
Non-alcoholic fatty liver disease (NAFLD) contributes to the global epidemic of metabolic syndrome and is considered a prelude end-stage diseases such as cirrhosis hepatocellular carcinoma. During NAFLD pathogenesis, hepatic parenchymal cells (hepatocytes) undergo both morphological functional changes owing rewired transcriptome. The underlying mechanism not entirely clear. In present study, we investigated involvement early growth response 1 (Egr1) in NAFLD.Quantitative PCR, Western...