A5102012871- Estrogen and related hormone effects
- Nuclear Receptors and Signaling
- Retinoids in leukemia and cellular processes
- Apelin-related biomedical research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Bone Metabolism and Diseases
- Reproductive System and Pregnancy
- Retinal Development and Disorders
- Lipid metabolism and disorders
- Inflammatory mediators and NSAID effects
- Diabetes Treatment and Management
- Ocular Diseases and Behçet’s Syndrome
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Menopause: Health Impacts and Treatments
- Testicular diseases and treatments
- Cytokine Signaling Pathways and Interactions
- Advanced biosensing and bioanalysis techniques
- Pulmonary Hypertension Research and Treatments
- Hormonal Regulation and Hypertension
- Urological Disorders and Treatments
- TGF-β signaling in diseases
- Endometriosis Research and Treatment
- Sexual Differentiation and Disorders
- Neurogenesis and neuroplasticity mechanisms
Guiyang Medical University
2025
Hubei University of Arts and Science
2025
Xiangyang Central Hospital
2025
Nanjing Normal University
2015-2024
First Hospital of Jilin University
2022-2023
Jilin University
2022-2023
China Medical University
2012-2022
Shanxi Medical University
2014-2022
Fourth Affiliated Hospital of China Medical University
2012-2022
Nanyang Technological University
2022
We present evidence that retinoic acid can down-regulate transcriptional activation by the nuclear protooncogene c-jun. All three members of receptor (RAR) subfamily (RAR alpha, RAR beta, and gamma) repress induction human collagenase gene or a heterologous promoter contains AP-1-binding site. In contrast, retinoid X fails to Jun/AP-1 activity, demonstrating significant difference between two regulatory systems through which retinoids exert their control. Analysis alpha mutants in...
Estrogen deficiency causes bone loss, which can be prevented by estrogen replacement therapy. Using a recently developed technique for isolation of highly purified mammalian osteoclasts, we showed that 17 β-estradiol (E2) was able to directly inhibit osteoclastic resorption. At concentrations effective inhibiting resorption, E2 also induced osteoclast apoptosis in dose- and time-dependent manner. ICI164,384 tamoxifen, as pure partial antagonists, respectively, completely or partially blocked...
17β-Estradiol modulates gene transcription through the estrogen receptor and response element in DNA. The human transforming growth factor-β3 was shown to be activated by presence of metabolites or antagonists. Activation mediated a polypurine sequence, termed raloxifene element, did not require DNA binding domain receptor. Interaction with appears cellular adapter protein. observation that individual estrogens modulate multiple elements may explain tissue-selective agonist antagonist...
Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30-200 nm) poses serious challenges in their isolation from complex media containing a variety extracellular vesicles (EVs) different sizes, especially sample volumes. Here we present viscoelasticity-based microfluidic system directly separate cell culture or serum continuous, size-dependent, and label-free...
Interaction of the estrogen receptor/ligand complex with a DNA response element is known to regulate gene transcription. In turn, specific conformations receptor-ligand have been postulated influence unique subsets estrogen-responsive genes resulting in differential modulation and, ultimately, tissue-selective outcomes. The receptor ligands raloxifene and tamoxifen demonstrated such tissue-specific agonist/antagonist effects. Both agents antagonize effects on mammary tissue while mimicking...
Apela (APJ early endogenous ligand, also known as elabela or toddler) is a recently discovered peptide hormone. Based on genetic studies in zebrafish, apela was found to be important for endoderm differentiation and heart development during embryogenesis. Although common phenotypes of APJ-null zebrafish embryonic suggested that interacts with the APJ receptor, kinetics binding intracellular signaling pathways remain unknown. The role adults uncertain. Using chimeric we showed direct high...
In this report, we demonstrate that, in contrast to most previously characterized nuclear receptors, hERR1 and hERR2 (human estrogen receptor-related protein 1 -2) are constitutive activators of the classic response element (ERE) as well palindromic thyroid hormone (TREpal) but not glucocorticoid (GRE). This intrinsically activated state resides ligand-binding domains two genes is transferable a heterologous receptor. addition, show that members p160 family receptor coactivators, ACTR...
Estrogen or raloxifene (LY156758) prevent estrogen deficiency-induced bone loss in animals and humans. We demonstrated the rat that a 22% reduction mineral density generated by ovariectomy was associated with 2-fold of transforming growth factor-beta 3 (TGF beta 3) messenger RNA expression femur. Administration 17 beta-estradiol to ovariectomized rats restored both TGF femur levels measured intact animals. In transient transfection assays, promoter sequence from -38 + 110 human gene, which...
The 2-arylbenzothiophene raloxifene, 1, is a selective estrogen receptor modulator (SERM) which currently under clinical evaluation for the prevention and treatment of postmenopausal osteoporosis. In vivo structure−activity relationships molecular modeling studies have indicated that orientation basic amine-containing side chain relative to stilbene plane, an important discriminating factor maintenance tissue selectivity. We constructed series analogues 1 in this held orthogonal similar...
High-level expression of the full-length human retinoic acid receptor (RAR) alpha and DNA binding domain RAR in Escherichia coli was achieved by using a T7 RNA polymerase-directed system. After induction, protein produced at an estimated level 20% total bacterial proteins. Both intact molecules bind to cognate response element with high specificity absence acid. However, this is enhanced great extent upon addition eukaryotic cell extracts. The factor responsible for enhancement...
Increasing studies have demonstrated that ginsenoside Rg3 (Rg3) plays an important role in the prevention and treatment of various diseases, including allergic lower airway inflammation such as asthma. To investigate upper disease, effect therapeutic mechanism rhinitis (AR) were studied. Ovalbumin-induced AR model mice intragastrically administered with Rg3. Nasal symptoms, levels IgE, IL-4, IL-5, IL-13, SOD MDA serum, histopathological analysis nasal mucosa used to evaluate on ameliorating...
Objective: To evaluate the effectiveness of cardiac rehabilitation therapy in treatment patients with chronic heart failure (CHF). Methods: 76 CHF who were treated hospital from January 2023 to December 2024 selected and randomly divided into two groups using a random number table. The experimental group (38 patients) received therapy, while reference conventional drug therapy. total effective rate, function indicators, lung pulse wave velocity (PWV) compared between groups. Results: rate...
Yang, Na N.; Venugopalan, Murali; Hardikar, Sushant; Glasebrook, Andrew Author Information
Abstract Physical exercise-induced enhancement of learning and memory alleviation age-related cognitive decline in humans have been widely acknowledged. However, the mechanistic relationship between exercise improvement remains largely unknown. In this study, we found that exercise-elicited benefits were accompanied by adaptive hippocampal proteasome activation. Voluntary wheel running increased activity adult middle-aged mice, contributing to an acceleration neurogenesis could be reversed...
Plant viruses cause severe losses in agricultural production. Parasite-derived resistance (PDR) offers a promising avenue for developing disease-resistant varieties independent of genes. However, potyviruses with great importance, such as soybean mosaic virus (SMV), systematic research on viral genes that can be used PDR has not been conducted. In this study, we transiently expressed the untranslated region (UTR) or each protein-coding cistron SMV Nicotiana benthamiana to evaluate their...