A5100340895- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Diabetes and associated disorders
- MicroRNA in disease regulation
- Diabetes Management and Research
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Cholesterol and Lipid Metabolism
- Diabetes Treatment and Management
- Music Therapy and Health
- Nitric Oxide and Endothelin Effects
- Stoma care and complications
- Cancer Genomics and Diagnostics
- Diet, Metabolism, and Disease
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Microgrid Control and Optimization
- Signaling Pathways in Disease
- Neurological diseases and metabolism
- Liver physiology and pathology
- Electron Spin Resonance Studies
- Fetal and Pediatric Neurological Disorders
- Pharmacological Receptor Mechanisms and Effects
- Cardiovascular Syncope and Autonomic Disorders
- Nicotinic Acetylcholine Receptors Study
Dezhou University
2025
Qilu Hospital of Shandong University
2025
Children's Hospital of Zhejiang University
2024
Tianjin Medical University General Hospital
2019-2022
China Pharmaceutical University
2016-2022
Inner Mongolia University
2021
Nanjing Medical University
2021
Shanghai Institute of Materia Medica
2015
National Center for Drug Screening
2015
Guilin Medical University
2015
The LIM domain homeobox gene islet 1 (isl-1) is expressed in the embryonic nervous system and may be an early marker of motor neuron specification. isl-1 all 4 cell types, but a role for regulation insulin expression has not been demonstrated, genetic targets pancreas remain unknown. We show here that proximal rat proglucagon promoter binds amino-terminally truncated Trp-E-isl-1 fusion protein lacks domains. also full-length vitro translated containing intact antisera detects binding...
Monogenic diabetes, which encompasses neonatal diabetes (NDM), maturity onset of the young (MODY), and several diabetes-associated syndromes, primarily arises from impaired function or abnormal development islets Langerhans, particularly pancreatic β-cells responsible for insulin secretion. This condition is typically associated with a single pathogenic genetic mutation. Charcot-Marie-Tooth disease type 1A (CMT1A) hereditary demyelinating neuropathy that caused by duplication PMP22 gene...
Progressive loss and dysfunction of islet β-cells has not yet been solved in the treatment diabetes. Regenerating protein (Reg) identified as a trophic factor which is demonstrated to be associated with pancreatic tissue regeneration. We previously produced recombinant Reg3α (rReg3α) proved that it protects against acute pancreatitis mice. Whether rReg3α diabetes elusive. In present study, stimulated MIN6 cell proliferation resisted STZ-caused death. The protective effect was also found...
Abstract Regenerating genes (Reg) have been found during the search for factors involved in pancreatic islet regeneration. Our recent study discovered that β-cell-specific overexpression of Reg3β protects against streptozotocin (Stz) -induced diabetes mice. To investigate its potential roles treatment diabetes, we produced a recombinant protein and provided evidence it is active promoting β-cell survival Stz- triggered cell death. Though ineffective alleviating preexisting pretreatment was...
Reg family proteins such as Reg1 and islet neogenesis-associated protein (INGAP) have long been implicated in the growth and/or neogenesis of pancreatic cells. Recent reports further suggest similar roles to be played by new members Reg2, Reg3α, Reg3β. We studied their age-, isoform-, tissue-specific expressions. RNA were isolated from C57BL/6 mice aged 7, 30, 90 days. Using real-time polymerase chain reaction, levels gene expression pancreas 20-600-fold higher than that other tissues...
The source of insulin-secreting cells from adult duct system is attractive, but its clinical practice remains poorly understood. Here, we aimed at identifying the distribution secreted hormone reactive in ducts.Consecutive pancreatic slices nondiabetic subjects were subjected to immunohistochemistry and immunofluorescence screen islet hormones (insulin; glucagon, Glu; somatostatin, Som; polypeptide, PP) exocrine biomarkers (cytokeratin 19, CK19; chromogranin A, CgA; amylase). All sections...
Aims The potential effect of regenerating (Reg) proteins in the treatment diabetes has been indicated past decade, but clinical use Reg requires more advances translational medicine. In present study, we produced recombinant protein 2 (rReg2), to prove its protective against streptozocin (STZ)‐induced BALB/c mice. Materials and Methods rReg2 was administrated STZ‐induced diabetic Blood glucose, body weight, serum insulin islet β‐cell loss were determined. However, Reg2 also reported serve as...
Abstract Context Evidence indicates that there is substantial impairment/loss of β-cell function/mass even before prediabetes. Elevated plasma proinsulin a sign dysfunction in patients with diabetes/prediabetes. However, the dynamic changes glucose stimulated secretion (GSPS) among nondiabetic individuals remain obscure. Objective To examine GSPS and glucose-stimulated insulin (GSIS) normal tolerance (NGT) impaired (IGT) to evaluate whether an early biomarker impairment NGT who have...
Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing (IPCs), which can be used treat type I diabetes. miR-375 is an essential transcriptional regulator in development and maturation of pancreas. In this study, we optimized a protocol hESCs IPCs successfully obtained IPCs. Then, performed overexpression inhibition experiments on at different stages differentiation RNA-seq. The results showed that expression fluctuated during hESC was affected by...
MicroRNA (miR)‑770‑5p expression is increased in patients with type 2 diabetes mellitus (T2DM) compared healthy controls; however, the roles and molecular mechanism underlying miR‑770‑5p T2DM are not completely understood. In present study, reverse transcription‑quantitative PCR (RT‑qPCR) results indicated that was significantly Bcl‑2 associated athanogene 5 (BAG5) decreased serum of volunteers. TargetScan a dual luciferase reporter gene system were used to predict verify BAG5 as target...
Genomic disorders caused by pathogenic copy number variation (pCNV) have proven to underlie a significant proportion of birth defects. With technological advance, improvement bioinformatics analysis procedure, and accumulation clinical data, non-invasive prenatal screening pCNV (NIPS-pCNV) high-throughput sequencing maternal plasma cell-free DNA has been put use in settings. Specialized standards for application NIPS-pCNV are required. Based on the discussion, 10 pCNV-associated diseases...
Neurogenic orthostatic hypotension with Parkinson's disease as a cause of syncope: A case report
<div>Abstract<p>Menin is a tumor suppressor protein mutated in patients with multiple endocrine neoplasia type 1. We show that menin essential for canonical Wnt/β-catenin signaling cultured rodent islet cells. In these cells, overexpression of significantly enhances <i>TCF</i> gene assay reporter activity response to β-catenin activation. Contrastingly, inhibition expression <i>Men1</i> siRNA decreases TCF activity. Likewise, 1 disease associated missense...
<div>Abstract<p>Menin is a tumor suppressor protein mutated in patients with multiple endocrine neoplasia type 1. We show that menin essential for canonical Wnt/β-catenin signaling cultured rodent islet cells. In these cells, overexpression of significantly enhances <i>TCF</i> gene assay reporter activity response to β-catenin activation. Contrastingly, inhibition expression <i>Men1</i> siRNA decreases TCF activity. Likewise, 1 disease associated missense...
Supplementary Figures S1-S3 from Menin Promotes the Wnt Signaling Pathway in Pancreatic Endocrine Cells
Supplementary Figures S1-S3 from Menin Promotes the Wnt Signaling Pathway in Pancreatic Endocrine Cells