A5100456226- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Gestational Diabetes Research and Management
- Prenatal Screening and Diagnostics
- Maternal and fetal healthcare
- RNA modifications and cancer
- Pregnancy and Medication Impact
- Preterm Birth and Chorioamnionitis
- Extracellular vesicles in disease
- Congenital Diaphragmatic Hernia Studies
- Macrophage Migration Inhibitory Factor
- Adenosine and Purinergic Signaling
- Epigenetics and DNA Methylation
- Renal and related cancers
- Apelin-related biomedical research
- Autophagy in Disease and Therapy
- Vitamin D Research Studies
- Toxoplasma gondii Research Studies
- Endometriosis Research and Treatment
- Congenital Anomalies and Fetal Surgery
- Human Health and Disease
Qingdao University
2010-2024
Affiliated Hospital of Qingdao University
2010-2024
Jilin University
2024
Chongqing Medical University
2024
Chongqing Public Health Medical Center
2024
Hengshui University
2023
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
2023
Shaoxing People's Hospital
2023
First Affiliated Hospital of Bengbu Medical College
2022
Second Xiangya Hospital of Central South University
2022
Smoking is a major cause of morbidity and mortality. Smoking-related epigenetic biomarkers may open new avenues to better quantify the adverse health effects smoking, understanding underlying mechanisms. We aimed evaluate clinical implications F2RL3 methylation, novel biomarker smoking exposure disclosed by recent genome-wide methylation studies.Blood DNA at (also known as PAR-4) was quantified in baseline samples 3588 participants aged 50-75 years large population-based prospective cohort...
Background: Histone lactylation, a novel epigenetic modification induced by hypoxia and lactate, plays an important role in regulating gene expression. However, the of histone lactylation pathogenesis preeclampsia remains unknown. Methods: Placentas from preeclamptic patients control pregnant women were collected for protein immunoassay to detect expression level two trophoblast cell lines used simulate effect on genes. Results: We found that lactate levels increased placentas. In vitro, was...
Human amnion-derived mesenchymal stem cells (AD-MSCs) have been reported as a promising effective treatment to repair tissue. Trophoblast dysfunction during pregnancy is significantly involved in the pathogenesis of preeclampsia (PE). To understand how AD-MSCs regulated trophoblast function, we treated trophoblasts with AD-MSC-derived exosomes under hypoxic conditions. The markedly enhanced proliferation and autophagy. Furthermore, significant decrease EZH2 levels inactivation mTOR signaling...
Abstract Background Decidual macrophages (dMφ) are pivotal in maintaining maternal–fetal immune tolerance during normal pregnancy by expressing a range of immune-suppressive molecules, including CD73. It has been demonstrated that Toxoplasma gondii ( T . ) infection can impair dMφ function, potentially leading to adverse outcomes, through downregulation these inhibitory molecules. rhoptry protein 18 Tg ROP18), key virulence factor , is associated with the incapacitation host’s innate and...
Early-onset preeclampsia (EOS-PE) refers to that occurred before 34 gestation weeks. This study is conducted explore the relationship between mitochondrial dysfunction and pathogenesis of EOS-PE using proteomic strategy.To identify altering expressed proteins severe healthy pregnancies, enrichment mitochondria coupled with iTRAQ-based quantitative method performed. Immunohistochemistry (IHC) western blot are performed detect alteration changing expression proteins, confirmed accuracy...
Preeclampsia (PE) is a specific disorder of pregnancy with significant morbidity and mortality to the mother fetus. It has been reported that abnormal regulation trophoblast cells contributes development PE. LIM SH3 Protein 2 (LASP2) belongs nebulin protein family actin-binding broad biological functions. In this study, we evaluated role LASP2 in cells. Our results demonstrated was markedly up-regulated placentas patients Overexpression significantly suppressed cell proliferation, migration,...
ABSTRACT: Aim: This study was performed to address the bone injury and early molecular responses of obstructive nephropathy induced by unilateral ureteral obstruction in mice. Methods: The male mice were subjected (UUO, n = 10) or sham operation ( 10). All killed on day 7 after surgical operation. Hematoxylin eosin tartate‐resistant acid phosphatase staining paraffin‐embedded sections. Expression genes proteins analyzed reverse transcription‐polymerase chain reaction, Western blotting...
Preeclampsia (PE), a pregnancy disorder that affects 5-7% of pregnant women, is among the primary causes for maternal and perinatal mortality. PE believed to be associated with insufficient invasion villous extravillous trophoblasts (EVTs), which hampers uterine spiral artery remodeling finally induces PE. But mechanism responsible reduction trophoblast remains unclear. In this study, placental tissues taken from healthy donors patients were used evaluate miR-326 expression; CCK8 colony...
This study measured the serum levels of complement component (C)3a and C5a placental expressions C3a receptor (R) C5aR to determine a potential correlation with circulating angiotensin II type 1 (AT1) agonistic autoantibody (AT1-AA) in severe pre-eclampsia.A total 118 women were recruited divided into 2 groups: control group (normotensive preterm pregnancies, n = 66) pre-eclampsia (n 52). Levels C3a, AT1-AA by enzyme-linked immunosorbent assay C3aR placenta Western blotting.Levels from...
Magnesium sulfate (MgSO
Preeclampsia (PE) is associated with oxidative stress in the maternal circulation and placenta. This study aimed to determine if inhibition of lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) gives protection against stress-mediated trophoblast dysfunction.Plasma placenta samples were obtained from 106 women PE normal pregnancy (NP). Oxidized (oxLDL) soluble LOX-1 levels determined by enzyme-linked immunoassay. Placental expression was western blotting. Trophoblasts subjected...
Trophoblast dysfunction during pregnancy is fundamentally involved in preeclampsia. Several studies have revealed that human chorionic villous mesenchymal stem cells (CV-MSCs) could regulate trophoblasts function. To understand how trophoblast function, we treated with CV-MSC supernatant under hypoxic conditions. Treatment markedly enhanced proliferation and invasion augmented autophagy. Transcriptome pathway analyses of before after treatment JAK2/STAT3 signalling as an upstream regulator....
The pathogenesis of preeclampsia (PE) is associated with inflammation and endothelial damage. Ulinastatin (UTI) mainly inhibits proteolytic activity significantly reduces the release interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-α) from macrophages. It also ameliorates vascular damage in pathological conditions. Hence, we investigated effects UTI a rat model PE induced using N(gamma)-nitro-l-arginine methyl ester (L-NAME).Although inducing model, 5000 U/kg were injected daily....
To establish a model for predicting adverse outcomes in advanced-age pregnant women with preterm preeclampsia China.We retrospectively collected the medical records of 896 who were older than 35 years and delivered at Affiliated Hospital Qingdao University from June 2018 to December 2020. The divided into an outcome group non-adverse according occurrence outcomes. data training set verification ratio 8:2. A nomogram was developed binary logistic regression created predict preeclampsia. ROC...
Human chorionic villous mesenchymal stem cells (CV-MSCs) are a promising and effective therapeutic option for tissue injury. Vascular dysfunction during pregnancies is significantly involved in the pathogenesis of preeclampsia (PE). This work aims to investigate how CV-MSCs regulate function vascular endothelial cells. In this study, RNA-seq analysis was used examine changes HUVECs treated with CV-MSC conditioned medium (CM). We examined levels ABCA9 AKT signaling human umbilical vein...
Uterine leiomyomas are common clinical gynecological tumors, which a major health concern for many women. In the current study, we aimed to investigate effect of paclitaxel (PTX) on uterine leiomyomas. A mouse model was established by estradiol benzoate, followed treatment with increasing doses PTX. PTX showed no dose-limiting toxicity that affected survival mice, and able restore apoptosis level uterus tissues mice normal levels. this discovered played critical role in promoting leiomyomas,...