A5088892345- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Traumatic Brain Injury and Neurovascular Disturbances
- Cell death mechanisms and regulation
- Advanced Proteomics Techniques and Applications
- Mitochondrial Function and Pathology
- Immune cells in cancer
- Neonatal and fetal brain pathology
- S100 Proteins and Annexins
- Amino Acid Enzymes and Metabolism
- MicroRNA in disease regulation
- Neurofibromatosis and Schwannoma Cases
- Kruppel-like factors research
- Cancer Mechanisms and Therapy
- TGF-β signaling in diseases
- Thermal Regulation in Medicine
- Acupuncture Treatment Research Studies
- Galectins and Cancer Biology
- Gastric Cancer Management and Outcomes
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Epigenetics and DNA Methylation
- Spinal Cord Injury Research
- Wnt/β-catenin signaling in development and cancer
- Cancer Research and Treatments
- Metabolism and Genetic Disorders
Tianjin Medical University Cancer Institute and Hospital
2022-2025
National Clinical Research
2024-2025
Zhongda Hospital Southeast University
2024
Logistics University of People's Armed Police Force
2017-2021
Chinese People's Armed Police Force
2017-2021
Beijing Proteome Research Center
2012-2016
Anhui Medical University
2016
National Institutes for Food and Drug Control
2012
Necroptosis, a novel type of programmed cell death, is involved in stroke-induced ischemic brain injury. Although studies have sought to explore the mechanisms necroptosis, its signaling pathway has not yet be completely elucidated. Thus, we used oxygen-glucose deprivation (OGD) and middle cerebral artery occlusion (MCAO) models mimicking stroke (IS) conditions investigate necroptosis. We found that OGD MCAO induced local ischemia neurological deficit, while zVAD-fmk (zVAD, an apoptotic...
Traumatic spinal cord injury (SCI) causes neuronal death, demyelination, axonal degeneration, inflammation, glial scar formation, and cystic cavitation resulting in interruption of neural signaling loss nerve function. Multifactorial targeted therapy is a promising strategy for SCI.The anti-inflammatory peptide KAFAKLAARLYRKALARQLGVAA (KAFAK) brain-derived neurotrophic factor (BDNF)-modified hyaluronan-methylcellulose (HAMC) hydrogel was designed minimally invasive, localized, sustained...
Rationale:The heterogeneity of tumor cells within the glioblastoma (GBM) microenvironment presents a complex challenge in curbing GBM progression.Understanding specific mechanisms interaction between different cell subclusters and non-tumor is crucial.Methods: In this study, we utilized comprehensive approach integrating glioma single-cell spatial transcriptomics.This allowed us to examine molecular interactions localization GBM, focusing on subcluster, subcluster 6, M2-type tumor-associated...
Glioblastoma (GBM) resistance to chemoradiotherapy is a major factor contributing poor treatment outcomes. This markedly affects the effectiveness of surgery combined with and leads post-surgical tumor recurrence. Therefore, exploring mechanisms underlying in GBM crucial for understanding its progression improving therapeutic options. study found that moesin (MSN) acts as key promotor glioma stem cells (GSCs), enhancing their proliferation stemness maintenance. Mechanistically, MSN activates...
<title>Abstract</title> G protein-coupled receptors (GPCRs) play a pivotal role in maintaining the stemness of both normal and cancer stem cells. However, function regulator protein signaling (RGS) family, particularly tumor cells, remains poorly understood. Through bioinformatics analysis clinical data, we identified RGS20 as potential glioma temozolomide (TMZ) resistance, which may significantly influence patient prognosis. Subsequent vitro vivo experiments demonstrated that inhibition...
Hepatitis B virus X protein (HBx) participates in the occurrence and development processes of hepatocellular carcinoma (HCC) as a multifunctional regulation factor. However, underlying molecular mechanism remains obscure. Here, we describe use p21HBx/+ mouse SILAM (Stable Isotope Labeling Mammals) strategy to define pathological mechanisms for HBx induced liver cancer. We systematically compared series proteome samples from regular mice, 12- 24-month old mice representing inflammation HCC...
Glioma is one of the most malignant forms brain tumor, and has been persistent concern due to its high recurrence mortality rates, limited therapeutic options. As a cardiac glycoside, ouabain widespread applications in congestive heart diseases positive inotropic effect by inhibiting Na+/K+‑ATPase. Previous studies have demonstrated that antitumor activity several types human including glioma. However, exact underlying mechanism remains be elucidated. The purpose present study was elucidate...
The launch of the Chromosome-Centric Human Proteome Project provides an opportunity to gain insight into human proteome. Chinese Chromosome Consortium has initiated proteomic exploration protein-encoding genes on chromosomes 1, 8, and 20. Collaboration within consortium generated a comprehensive proteome data set using normal carcinomatous tissues from liver, stomach, colon 13 cell lines originating in these organs. We identified 12,101 proteins (59.8% coverage against Swiss-Prot entries)...
Traumatic brain injury (TBI) contributes to hypocoagulopathy associated with prolonged bleeding and hemorrhagic progression. Bloodletting puncture therapy at hand twelve Jing-well points (BL-HTWP) have been applied as a first aid measure in various of emergent neurological diseases, but the detailed mechanisms modulation between central nervous system systemic circulation after acute TBI rodents remain unclear. To investigate whether BL-HTWP stimulation modulates hypocoagulable state exerts...
Background: Glioma is the most common malignant intracranial tumor with high lethality. Despite surgery combined chemoradiotherapy, prognosis for patients glioma remains poor. This primarily due to acquired chemoradiotherapy resistance. Therefore, improve of glioma, further study into mechanism resistance needed. Objective: aimed (1) evaluate by using a prognostic risk score model constructed genes, (2) provide new targets and directions precise treatment (3) discuss heterogeneity cells....
Objective . Casein kinase 2 interacting protein-1 (CKIP-1) has exhibited multiple functions in regulating cell proliferation, apoptosis, differentiation, and cytoskeleton. CKIP-1 also plays an important role as a critical regulator tumorigenesis. The aim of this study is to further examine the function glioma cells. Methods expression level protein was determined gliomas tissues lines by immunohistochemistry stain western blotting while association with prognosis analyzed Kaplan-Meier method...
To observe the effect of bloodletting acupuncture at twelve jing-well points hand on microcirculatory disturbance in mice with traumatic brain injury (TBI), and to explore protective therapy TBI.Sixty clean adult male C57BL/6J were randomly divided into a sham-operation group, model group treatment 20 each group. The TBI was established by using electronic controlled cerebral cortex impact instrument treated bilateral "Shaoshang" (LU 11), "Shangyang" (LI 1), "Zhongchong" (PC 9), "Guanchong"...
To analyze the efficacy of alternate titanium clip closure in preventing postoperative complications for patients with gastric mucosal lesions after endoscopic submucosal dissection (ESD).
Stromal cells in the tumor microenvironment play crucial roles glioma development. Current methods for isolating tumor-associated stromal (such as neutrophils) are inefficient due to conflict between tissue dissociation and cell surface protein protection, which hampers research on patient-derived cells. Our study aims establish a novel method glioma-associated neutrophils (GANs).To observe neutrophil-like polymorphonuclear cells, we performed Hematoxylin-Eosin staining tissues. For single...
SILAC (Stable Isotope Labeling with Amino acids in Cell culture) is one of the most popular methods quantitative proteomics field. The internal standards can mark a variety clinical samples or tissues instead labeling whole organism and play an important role proteomics. Preparing has been difficult time-consuming for culturing cell lines, therefore simple convenient Re-SILAC method was introduced. lines labeled were thawed medium to be remarked again. Comparing morphology, size, growth...
Objective To investigate the effects of synuclein-γ (SNCG) gene silencing on proliferation and apoptosis glioma U87-MG cells. Methods Five small hairpin RNA templates targeting SNCG a negative control were synthesized cloned into lentiviral vector system all constructs sequenced. Then recombinant vectors used to infect cells. The lentiviruses which can effectively inhibit protein expression levels selected by RT-PCR for further study. Colony formation flow cytometry assay...
Objective To investigate the effects of necroptosis induced by receptor-interacting protein 3 (RIP3) on stretch injury model HT-22 cells and its mechanisms. Methods HT-22 cell lines were seeded with Bioflex plate, injuries 30 psi for regulator a 50 msec pulse resulting in 3.5~4.5 peak pressure controller Ⅱ (CIC) instrument. The morphological changes assessed digital holographic microscopy (DHM), degree was detected lactate dehydrogenase (LDH) assay, cycle Flow cytometry expression...
Objective To investigate the effects of necroptosis induced by receptor interacting-protein 3 (RIP3) on traumatic brain injury (TBI) model C57BL/6 mice and its mechanisms. Methods C57BL/6 were positioned beneath controlled cortical impactor device (CCI) subjected to impact at 1 mm depth penetration, for a sustained depression 120 msec velocity 5 m/s. Ctrl group was not treated. Sham received craniotomy, without CCI injury. TBI 4 μl DMSO injected. GSK’872 Then, degree detected modified...
Objective To establish the cell injury model with mechanical loading system in HT-22 lines. Methods HT-22 lines were seeded Bioflex plate, and applied tensile stress-induced at degree of deformation 13.4% frequency 1 Hz by FX-5000TFL system. cells divided into control, 6 h, 12 24 h group, respectively. The mean area thickness assessed real-time digital holographic microscopy (DHM), Trypan blue assay lactate dehydrogenase (LDH) detected kit then performed to determine viability level...
The refractory epilepsy refers to the whose seizures couldn’t be cured after using two kinds of correctly selected antiepileptic drugs which can tolerated and enough dosage duration monotherapy or combination therapy.The pathogenesis intractable is complex, there no established theory at home abroad.Recently, more attention has been paid correlation between mitochondrial dysfunction caused by oxidative stress epilepsy.Based on summary common treatment searching related literature abroad,...