A5081736725- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Hepatitis B Virus Studies
- Computational Drug Discovery Methods
- Extracellular vesicles in disease
- Hepatitis C virus research
- MicroRNA in disease regulation
- Microbial Metabolic Engineering and Bioproduction
- Liver physiology and pathology
- Carcinogens and Genotoxicity Assessment
- Superconducting Materials and Applications
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Cancer, Lipids, and Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
- Biotin and Related Studies
- Biometric Identification and Security
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- Effects and risks of endocrine disrupting chemicals
- Retinoids in leukemia and cellular processes
- Electromagnetic Launch and Propulsion Technology
Beijing Proteome Research Center
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2025
Chinese PLA General Hospital
2025
Shandong Academy of Sciences
2024
Qilu University of Technology
2024
Academy of Military Medical Sciences
2024
Xuzhou Medical College
2023
Sichuan University
2023
State Key Laboratory of Oral Diseases
2023
University of Alabama at Birmingham
2022
Abstract Runt-related transcription factor 1 (Runx1) plays a key role in cartilage formation, but its function articular formation is unclear. We generated non-inducible and inducible Runx1-deficient mice ( Runx1 f/f Col2α1-Cre Col2α1-CreER mice) found that chondrocyte-specific developed spontaneous osteoarthritis (OA)-like phenotype showed exacerbated destruction under OA, characterized by degradation ossification, with decreased Col2α1 expression increased Mmp13 Adamts5 expression....
The fundamental importance of the 26S proteasome in health and disease suggests that its function must be finely controlled, yet our knowledge about regulation remains limited. Posttranslational modifications, especially phosphorylation, subunits have been shown to impact through different mechanisms, although vast majority phosphorylation events not studied. Here, we characterized 1 most frequently detected phosphosites, namely Ser361 Rpn1, a base subunit 19S regulatory particle. Using...
Hepatocellular carcinoma (HCC) is the most common form of hepatic malignancies. The diagnosis HCC remains challenging due to low sensitivity and specificity diagnostic method. Exosomes, which are abundant in various proteins from parent cells, play pivotal roles intercellular communication have been confirmed as promising sources disease biomarkers. Herein, we performed a simple but robust proteomic profiling on exosomes derived 1 μL serum using data-independent acquisition (DIA) method for...
Although cathepsin S is transported from the spleen to liver, where it cleaves collagen XVIII produce endostatin and plays a critical role in onset of early liver fibrosis, relationship between fibrosis function remains underexplored. Given roles phosphorylation disease, understanding its regulatory mechanism crucial. Despite advances mass spectrometry enhancing phosphoproteomics, application limited by small clinical samples subtle protein changes. We optimized phosphoproteomic workflow,...
Background & Aims: Only a minority of patients could benefit from systemic therapy owing to the high heterogeneity HCC. Therefore, deeper understanding pathogenesis HCC is essential for precision therapy. Genomic and proteomic studies have enhanced our However, phosphoproteomic characterization remains poorly understood. Approach Results: We conducted an in-depth analysis clinical cohort using high-coverage phosphoproteomic. Effective therapeutic targets were validated liver cancer cell...
Gap junctions, which are mainly composed of connexin units, play an indispensable role in cell morphogenesis, proliferation, migration, adhesion and differentiation osteoblast lineage cells, thus mediate bone development, homeostasis disease occurrence. Platelet-derived growth factor-AA (PDGF-AA) is proved to have a great influence on lines widely applied the field defect wound healing. However, PDGF-AA gap junction formation remains elusive. In current study, we aimed investigate impact...
As a hepadnavirus, hepatitis B virus (HBV) can cause damage to extrahepatic organs. The kidney is one of the organs that more susceptible damage. Research studies on HBV-associated glomerulonephritis (HBV-GN) have been going for decades. However, underlying molecular mechanism remains obscure. Here, we applied tandem mass tag (TMT) isobaric labeling-based method quantitatively profile proteome HBV transgenic mice illustrate pathological mechanisms HBV-GN. Weighted correlation network...
Triple-negative breast cancer (TNBC) is the most malignant subtype of cancer. Currently, chemotherapy remains to be primary treatment for TNBC, but drug resistance common while patient prognosis poor. With development proteomics technology, phosphoproteomics research has made great progress and been widely used in study tumor mechanism, diagnosis treatment. Similarly, plays a significant role studies occurrence, development, targeted therapy, mechanisms TNBC. This article summarizes with aim...
Abstract Next-generation risk assessment for environmental chemicals and ingredients in consumer products involves a weight of evidence (WoE) framework integrating suite new approach methodologies (NAMs) based on points departure (PoD) obtained from vitro assays. Omics techniques provide broad coverages the molecular toxicity pathway space. Transcriptomics assays especially play leading role by providing relatively conservative PoDs comparison with apical endpoints. However, it is unclear...
ABSTRACT Omic-based technologies are of particular interest and importance for non-animal chemical hazard risk characterization based on the premise that any apical endpoint change must be underpinned by some alterations measured at omic levels. In this work we studied cellular responses to caffeine coumarin generating integrating multi-omic data from transcriptomic, proteomic phosphoproteomic experiments. We have shown methodology presented here is able capture complete chain events first...