A5100375875- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Phagocytosis and Immune Regulation
- Microbial Community Ecology and Physiology
- Identification and Quantification in Food
- RNA regulation and disease
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Advanced Proteomics Techniques and Applications
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- interferon and immune responses
- Cancer-related molecular mechanisms research
- ATP Synthase and ATPases Research
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Pesticide Exposure and Toxicity
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2025
Beijing Proteome Research Center
2015-2025
Academy of Military Medical Sciences
2018-2025
First Affiliated Hospital of Wenzhou Medical University
2024
Wenzhou Medical University
2024
Peking University People's Hospital
2022-2024
Peking University
2022-2024
Jilin University
2024
Chinese Academy of Sciences
2018-2023
Institute of Oceanology
2019-2023
Abstract Pancreatic cancer is an aggressive disease with multiple biochemical and genetic alterations. Thus, a single agent to hit one molecular target may not be sufficient treat this disease. The purpose of study identify novel Hsp90 inhibitor disrupt protein-protein interactions its cochaperones for down-regulating many oncogenes simultaneously against pancreatic cells. Here, we reported that celastrol disrupted Hsp90-Cdc37 interaction in the superchaperone complex exhibit antitumor...
Abstract Background Comprehensive annotation and quantification of transcriptomes are outstanding problems in functional genomics. While high throughput mRNA sequencing (RNA-Seq) has emerged as a powerful tool for addressing these problems, its success is dependent upon the availability quality reference genome sequences, thus limiting organisms to which it can be applied. Results Here, we describe Rnnotator, an automated software pipeline that generates transcript models by de novo assembly...
The molecular chaperone heat shock protein 90 (Hsp90) is required for the stabilization and conformational maturation of various oncogenic proteins in cancer. loading kinases to Hsp90 actively mediated by cochaperone Cdc37. crucial role Hsp90-Cdc37 complex has made it an exciting target cancer treatment. In this study, we characterize Cdc37 interaction drug disruption using a reconstituted system. GST pull-down assay ELISA show that binds ADP-bound/nucleotide-free but not ATP-bound Hsp90....
Using a high-throughput screening (HTS) approach, we have identified and validated several small-molecule Mcl-1 inhibitors (SMI). Here, describe novel selective SMI inhibitor, 2 (UMI-77), developed by structure-based chemical modifications of the lead compound 1 (UMI-59). We characterized binding UMI-77 to using complementary biochemical, biophysical, computational methods determined its antitumor activity against panel pancreatic cancer cells an in vivo xenograft model. binds BH3-binding...
Osteosarcoma is the most common primary bone cancer and notorious for pulmonary metastasis, representing a major threat to pediatric patients. An effective drug targeting osteosarcoma its lung metastasis urgently needed.In this study, sarcoma-targeting peptide-decorated disulfide-crosslinked polypeptide nanogel (STP-NG) was exploited enhanced intracellular delivery of shikonin (SHK), an extract medicinal herb, inhibit progression with minimal systemic toxicity.The targeted, loaded nanogel,...
Abstract SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in humans, and olfactory dysfunction is one the most predictive common symptoms COVID-19 patients. However, underlying mechanism by which leads to disorders remains elusive. Herein, we demonstrate that intranasal inoculation with induces robust viral replication epithelium (OE), not bulb (OB), resulting transient humanized ACE2 (hACE2) mice. The sustentacular cells Bowman’s gland OE were identified as major...
5-Methylcytosine (m5C), an abundant RNA modification, plays a crucial role in regulating fate and gene expression. While recent progress has been made understanding the biological roles of m5C, inability to introduce m5C at specific sites within transcripts hindered efforts elucidate direct links between phenotypic outcomes. Here, we developed CRISPR-Cas13d-based tool, named reengineered modification system (termed 'RCMS'), for targeted methylation demethylation transcripts. The RCMS editors...
Protein modification by ubiquitin and ubiquitin-like proteins (UBLs) regulates numerous biological functions. The UFM1 system, a novel UBL conjugation is implicated in mouse development hematopoiesis. However, its broad functions working mechanisms remain largely elusive. CDK5RAP3, possible ufmylation substrate, essential for epiboly gastrulation zebrafish. Herein, we report critical role of CDK5RAP3 liver hepatic Cdk5rap3 knockout mice displayed prenatal lethality with severe hypoplasia, as...
Apoptosis plays critical role in diabetic cardiomyopathy and endoplasmic reticulum stress (ERS) is one of intrinsic apoptosis pathways. For previous studies have shown that become swell myocardium ERS was involved diabetes mellitus heart failure, this study aimed to demonstrate whether induced streptozocin (STZ)-induced rats. We established type 1 rat model with STZ intraperitoneal injection, used echocardiographic evaluation, hematoxylin-eosin staining the terminal deoxynucleotidyl...
To estimate the potential of state-of-the-art proteomics technologies on full coverage encoding gene products, Chinese Human Chromosome Proteome Consortium (CCPC) applied a multiomics strategy to systematically analyze transciptome, translatome, and proteome same cultured hepatoma cells with varied metastatic qualitatively quantitatively. The results provide global view expression profiles. 9064 identified high confident proteins covered 50.2% all products in translatome. Those function...
A series of novel cyclopropylamide analogues combretastatin-A4 (CA-4) were designed and synthesized. Most them had significant in vitro antiproliferative activities, particularly for compounds 7i4, 7c4, 8a4, 8c4. Moreover, compound 8c4 was also equally potent against paclitaxel resistant cancer cells. Interestingly, the different binding mechanisms from CA-4. Instead inhibiting tubulin polymerization, these CA-4 derivatives able to stimulate polymerization. Flow cytometry revealed that...
// Xin-Li Liu 1, * , Xu-Tao Zhang Jin Meng 2, 3, Hong-Fei 1 Yong Zhao 4 Chen Li Yang Sun Qi-Bing Mei Feng and Tao 2 Key Laboratory of Gastrointestinal Pharmacology Chinese Materia Medica the State Administration Traditional Medicine, Department Pharmacology, School Pharmacy, Fourth Military Medical University, Xi'an, China Thoracic Surgery, Tangdu Hospital, 3 Pharmcy, Hospital PLA, Beijing, Animal Center, These authors contributed equally to this work Correspondence to: Zhang, email:...
Protein homeostasis is critical for cell survival and functions during stress regulated at both RNA protein levels. However, how the integrates RNA-processing programs with post-translational quality control systems unknown. Transactive response DNA-binding (TARDBP/TDP-43) an that involved in pathogenesis of major neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) frontotemporal dementia (FTD). Here, we report a conserved role TDP-43, from C. elegans to mammals,...
Protein quality control is essential for clearing misfolded and aggregated proteins from the cell, its failure associated with many neurodegenerative disorders. Here, we identify two genes, ufd-2 spr-5, that when inactivated, synergistically robustly suppress neurotoxicity in Caenorhabditis elegans. Loss of human orthologs ubiquitination factor E4 B (UBE4B) lysine-specific demethylase 1 (LSD1), respectively encoding a ubiquitin ligase demethylase, promotes clearance mammalian cells by...
Both caloric restriction (CR) and mitochondrial proteostasis are linked to longevity, but how CR maintains in mammals remains elusive. MicroRNAs (miRNAs) well known for gene silencing cytoplasm have recently been identified mitochondria, knowledge regarding their influence on function is limited. Here, we report that increases miRNAs, which required the CR-induced activation of translation, mouse liver. The ablation miR-122, most abundant miRNA induced by CR, or retardation biogenesis via...
Abstract Several groups of bacteria have complex life cycles involving cellular differentiation and multicellular structures. For example, actinobacteria the genus Streptomyces form vegetative hyphae, aerial spores. However, similar not yet been described for archaea. Here, we show that several haloarchaea family Halobacteriaceae display a cycle resembling bacteria. Strain YIM 93972 (isolated from salt marsh) undergoes into mycelia Other closely related strains are also able to mycelia,...
Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics testis tissue was a feasible approach to identify more MPs because tissues have higher gene expression levels. Here we combined proteomics and transcriptomics survey in human from three post-mortem individuals. Proteins were extracted separated with glycine- tricine-SDS-PAGE. A total 9597 protein groups identified; these, 166 listed as MPs, including 138 (83.1%)...
The genus Nocardiopsis is one of the most dominant Actinobacteria that survives in hypersaline environments. However, adaptation mechanisms for halophilism are still unclear. Here, we performed isobaric tags relative and absolute quantification based quantitative proteomics to investigate functions membrane proteome after salt stress. A total 683 proteins were identified quantified, which 126 displayed salt-induced changes abundance. Intriguingly, bioinformatics analyses indicated these...
The regulation of protein synthesis is essential for maintaining cellular homeostasis, especially during stress responses, and its dysregulation could underlie the development human diseases. critical step translation phosphorylation eukaryotic initiation factor 2 alpha (eIF2α). Here we report identification a direct kinase eIF2α, microtubule affinity-regulating (MARK2), which phosphorylates eIF2α in response to proteotoxic stress. activity MARK2 was confirmed cells lacking 4 previously...
Apoptosis plays a critical role in the diabetic cardiomyopathy, and endoplasmic reticulum stress (ERS) is one of intrinsic apoptosis pathways. Previous studies have shown that becomes swollen dilated myocardium, ERS involved heart failure kidney. This study aimed to demonstrate whether induced myocardium streptozotocin (STZ)-induced rats. We established type 1 rat model, used echocardiographic evaluation, hematoxylin–eosin staining, terminal deoxynucleotidyl transferase-mediated DNA nick-end...