A5090579121- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Microbial Metabolism and Applications
- Synthetic Organic Chemistry Methods
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Microbial Community Ecology and Physiology
- Mycobacterium research and diagnosis
- Marine Sponges and Natural Products
- Lipoproteins and Cardiovascular Health
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pharmacological Effects of Natural Compounds
- Bacterial biofilms and quorum sensing
- Plant biochemistry and biosynthesis
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Insect symbiosis and bacterial influences
- Synthesis and Biological Activity
Shanghai Normal University
2021-2025
Chinese Academy of Sciences
2013-2020
Center for Excellence in Molecular Plant Sciences
2013-2020
Shanghai Institutes for Biological Sciences
2013-2018
The RNA-guided DNA editing technology CRISPRs (clustered regularly interspaced short palindromic repeats)/Cas9 had been used to introduce double-stranded breaks into genomes and direct subsequent site-specific insertions/deletions or the replacement of genetic material in bacteria, such as Escherichia coli, Streptococcus pneumonia, Lactobacillus reuteri. In this study, we established a high-efficiency CRISPR/Cas9 genome plasmid pKCcas9dO for use Streptomyces manipulation, which comprises...
Streptomyces has a strong capability for producing large number of bioactive natural products and remains invaluable as source the discovery novel drug leads. Although Streptococcus pyogenes CRISPR-Cas9-assisted genome editing tool been developed rapid genetic engineering in Streptomyces, it limitations, including toxicity SpCas9 expression some important industrial strains need complex constructs when targeting multiple genomic loci. To address these problems, this study, we high-efficiency...
Streptomycetes are Gram-positive bacteria with the capacity to produce copious bioactive secondary metabolites, which main source of medically and industrially relevant drugs. However, genetic manipulation Streptomyces strains is much more difficult than other model microorganisms like Escherichia coli Saccharomyces cerevisiae. Recently, CRISPR/Cas9 or dCas9-mediated tools have been developed facilitated genome editing. till now, CRISPR/dCas9-based interference system (CRISPRi) only designed...
Streptomycetes have a strong ability to produce vast array of bioactive natural products (NPs) widely used in agriculture and veterinary/human medicine. The recently developed CRISPR/Cas9-based genome editing tools greatly facilitated strain improvement for target NP overproduction as well novel discovery Streptomyces. However, CRISPR/Cas9 shows high toxicity the host, limiting its application many Streptomyces strains with low DNA transformation efficiency. In this study, we low-toxicity...
Abstract Several groups of bacteria have complex life cycles involving cellular differentiation and multicellular structures. For example, actinobacteria the genus Streptomyces form vegetative hyphae, aerial spores. However, similar not yet been described for archaea. Here, we show that several haloarchaea family Halobacteriaceae display a cycle resembling bacteria. Strain YIM 93972 (isolated from salt marsh) undergoes into mycelia Other closely related strains are also able to mycelia,...
Efficient gene cluster editing tools are one of the key techniques for discovering novel compounds encoded by silent natural product (NP) biosynthetic clusters (BGCs) in microbial genomes. Currently, vivo BGC developed E. coli is most widely used, but they often introduces DNA scars into clusters, which may affect function target NP BGCs. Herein, a genome-integrated Cas9/λRed system-based scarless tool (iCASRED) was established BL23, constructed on basis BL21/DE3 with recA deletion and...
We previously demonstrated that in Streptomyces coelicolor two-component system AfsQ1/Q2 activates the production of yellow-colored coelimycin P2 (also named as yCPK) on glutamate-supplemented minimal medium, and response regulator AfsQ1 could specifically bind to intergenic region between two structural genes, cpkA cpkD. Here, a more in-depth investigation was performed elucidate mechanism underlying role regulating biosynthesis. Deletion afsQ1/Q2 resulted markedly decreased expression...
Pristinamycin, produced by Streptomyces pristinaespiralis, which is a streptogramin-like antibiotic consisting of two chemically unrelated components: pristinamycin I (PI) and II (PII), shows potent activity against many antibiotic-resistant pathogens. However, so far production titers are still quite low, particularly those PI. In this study, we constructed PI single component producing strain deleting the PII biosynthetic genes (snaE1 snaE2). Then, metabolic engineering approaches,...
There are up to seven regulatory genes in the pristinamycin biosynthetic gene cluster of Streptomyces pristinaespiralis , which infers a complicated regulation mechanism for production. In this study, we revealed that PapR6, putative atypical response regulator, acts as pathway-specific activator II (PII) biosynthesis. Deletion papR6 resulted significantly reduced PII production, and its overexpression led increased formation, compared parental strain HCCB 10218. However, either deletion or...
Pristinamycin I (PI), produced by Streptomyces pristinaespiralis, is a streptogramin type B antibiotic, which contains two proteinogenic and five aproteinogenic amino acid precursors. PI coproduced with pristinamycin II (PII), member of A antibiotics. The biosynthetic gene cluster has been cloned characterized. However, thus far little understood about the regulation biosynthesis. In this study, TetR family regulator (encoded SSDG_03033) was identified as playing positive role in Its...
In actinomycetes, two main regulators, the OmpR-like GlnR and TetR-type AmtR, have been identified as central regulators for nitrogen metabolism. GlnR-mediated regulation was previously in different actinomycetes except members of genus Corynebacterium, which AmtR plays a predominant role Interestingly, some (e.g. Streptomyces avermitilis) harbour both glnR- amtR-homologous genes chromosome. Thus, it will be interesting to determine how these types function together strains. this study,...
In Streptomyces coelicolor, amtB transcription is promptly regulated by the global nitrogen regulator GlnR. Although GlnR binding cis-element has been characterized in promoter, consisting of three boxes a3-b3, a1-b1 and a2-b2, its role GlnR-mediated transcriptional regulation remains unclear. Here, we showed that had different affinity against each pair sites promoter (i.e., a2-b2 sites), was able to bind a3-b3 a1-b1, respectively, but not alone. Consistently, a2 a typical site further...