A5064487981- RNA modifications and cancer
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- Environmental Impact and Sustainability
- Sustainability and Ecological Systems Analysis
- Reproductive System and Pregnancy
- Immune cells in cancer
- Retinal Development and Disorders
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- Pancreatitis Pathology and Treatment
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Retinal Diseases and Treatments
- Molecular Sensors and Ion Detection
- Nanoparticle-Based Drug Delivery
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- Retinopathy of Prematurity Studies
- Autism Spectrum Disorder Research
- Immune Cell Function and Interaction
- Land Use and Ecosystem Services
- Ovarian cancer diagnosis and treatment
Ruijin Hospital
2015-2024
Shanghai Jiao Tong University
2015-2024
North China University of Science and Technology
2023-2024
Nanjing Medical University
2024
XinHua Hospital
2017-2023
State Key Joint Laboratory of Environment Simulation and Pollution Control
2017-2023
Beijing Normal University
2017-2023
State Key Laboratory of Oncogene and Related Genes
2021-2023
Chinese PLA General Hospital
2022
China-Japan Friendship Hospital
2022
The immune microenvironment is crucial for epithelial ovarian cancer (EOC) progression and consists of tumor-associated macrophages (TAM) T lymphocytes, such as regulatory cells (Treg) helper 17 (Th17) cells. In this study, the Treg/Th17 ratio was significantly higher in EOC situ metastatic peritoneal tissues than benign tumors peritoneum. associated with histologic grade an independent prognostic factor overall survival patients. On basis microarray analysis exosomes derived from TAMs, we...
Cancer secreted exosomal miRNAs are emerging as mediators between tumor-stoma crosstalk. Here, we show epithelial ovarian cancer (EOC)-derived exosomes activated macrophages to a tumor-associated macrophage (TAM)-like phenotype with SOCS3/STAT3 pathway involvement, which could facilitate the progression of cancer. MiR-222-3p was enrichment in released from EOC cells and it be transferred macrophages. Overexpression miR-222-3p induced polarization M2 phenotype. Luciferase assay verified...
Recently, cancer has been considered to be a complex system that includes the tumor microenvironment (TME). Tumor-associated macrophages (TAMs) are most common immune-related stromal cells in TME, and communication between TAMs is crucial for progression of epithelial ovarian (EOC). In this study, we revealed exosomes derived from EOC remodel tumor-promoted phenotype, namely TAMs. addition, hypoxic microenvironments have postulated facilitate process hypoxia-inducible factors (HIFs) play an...
Pancreatic cancer, one of the top two most fatal cancers, is characterized by a desmoplastic reaction that creates dense microenvironment, promoting hypoxia and inducing epithelial-to-mesenchymal transition (EMT) to facilitate invasion metastasis. Recent evidence indicates long noncoding RNA NORAD may be potential oncogenic gene this lncRNA significantly upregulated during hypoxia. However, overall biological role clinical significance remains largely unknown. expression was measured in 33...
circRNA has been established to play a pivotal role in tumorigenesis development variety of cancers; nevertheless, the biological functions and molecular mechanisms hypoxia-induced exosomal circRNAs pancreatic cancer remain largely unknown.Differentially expressed exosomes between hypoxic normoxic PC cells were verified by RNA sequencing. The expression circPDK1 tumors patients was evaluated qRT-PCR ISH, through series vitro vivo experiments. Using Western blotting, Co-IP, pull-down, ChIP,...
Ovarian cancer (OC) is an aggressive gynecological tumor usually diagnosed with widespread metastases and ascites. Here, we depicted a single-cell landscape of the OC ecosystem five tumor-relevant sites, including omentum metastasis malignant Our data reveal potential roles ascites-enriched memory T cells as pool for tumor-infiltrating exhausted CD8+ helper 1-like cells. Moreover, tumor-enriched macrophages exhibited preference monocyte-derived ontogeny, whereas in ascites were more...
Heat shock transcription factorA2 (HsfA2) is a key regulator in response to heat stress Arabidopsis (Arabidopsis thaliana), and its (HS)-induced regulation has been extensively studied. Recently, alternative splicing, critical posttranscriptional event, shown regulate HS-inducible expression of HsfA2; however, the molecular mechanism remains largely unknown. Here, we demonstrate new stress-induced splice variant, HsfA2-III, involved self-regulation HsfA2 Arabidopsis. HsfA2-III generated...
Renal coloboma syndrome, also known as papillorenal syndrome is an autosomal-dominant disorder characterized by ocular and renal malformations. Mutations in the paired-box gene, PAX2, have been identified approximately half of individuals with classic findings hypoplasia/dysplasia abnormalities optic nerve. Prior to 2011, there was no actively maintained locus-specific database (LSDB) cataloguing extent genetic variation PAX2 gene phenotypic syndrome. Review published cases collective...
Rationale: Cisplatin derivatives are first-line chemotherapeutic agents for epithelial ovarian cancer. However, chemoresistance remains a major hurdle successful therapy and the underlying molecular mechanisms poorly understood at present. Methods: RNA sequencing of organoids (PDO) established from cisplatin-sensitive -resistant cancer tissue samples was performed. Glucose metabolism, cell senescence, chemosensitivity properties were subsequently examined. Immunoprecipitation, mass...
Long noncoding RNAs have been identified as key regulators in the progression of various cancers. LINC00261 has reported a tumor suppressor multiple However, its function and underlying mechanisms pancreatic cancer remain largely unclear. Quantitative real-time PCR was performed to detect RNA expression. In situ hybridization used discover subcellular location. The direct binding miR-222-3p verified using dual-luciferase reporter assay immunoprecipitation. LINC00261-binding proteins were...
Abstract Background Stanniocalcin 1 (STC1) plays an integral role in ovarian cancer (OC). However, the functional of STC1 metastasis, lipid metabolism and cisplatin (DDP) chemoresistance OC is not fully understood. Methods Single-cell sequencing IHC analysis were performed to reveal expression profiles patient tissues. Metastasis, DDP subsequently assessed. Cell-based vitro vivo assays conducted gain insight into underlying mechanism OC. Results verified that was significantly enhanced...
To study the mechanism by which epithelial ovarian cancer (EOC)-derived exosomes restore migration of endothelial cells that is suppressed TAM-derived exosomes.Exosomes were isolated from TAMs in ascites patients with EOC. The effect on expression cell miRNA was monitored PCR. mimics transfected to explore their effects. Microarray data and literature searches used predict target genes impact gene pathways, small interfering RNA these genes. We assays determine whether cell-derived...
Accumulating evidence has indicated that microRNAs (miRNAs) act as critical epigenetic regulators in tumor carcinogenesis. Here, we report miR‐199a‐3p was significantly upregulated gastric cancer (GC) cell lines and tissues. Functional studies demonstrated dramatically increased proliferation suppressed apoptosis both vitro vivo. Furthermore, the transcriptional regulator zinc fingers homeoboxes 1 ( ZHX1 ) identified one of direct downstream targets miR‐199a‐3p, bound to 3′ untranslated...
Abstract The National Ophthalmic Disease Genotyping and Phenotyping Network (eyeGENE ® ) was established in an effort to facilitate basic clinical research of human inherited eye disease. In order provide high quality genetic testing eyeGENE ’s enrolled patients which potentially aids diagnosis disease treatment, we carried out a pilot study performed Next-generation sequencing (NGS) based molecular for 105 Retinitis Pigmentosa (RP) randomly selected from the network. A custom capture panel...
To investigate and compare the preventive effects of apple polyphenols extract (APE) with phloretin on dextran sulfate sodium (DSS)‐induced ulcerative colitis (UC), 60 male mice were treated 125 or 500 mg/(kg bw d) APE 100 phloretin, single‐ingredient APE, for continuous 3 weeks by intragastric administration, meanwhile, provided 3% DSS dissolved in drinking water to induce UC during third week. Both significantly ameliorated DSS‐induced inhibiting body weight loss, preventing colon...
Our previous study showed that apple polyphenol extract (APE) ameliorated high-fat diet-induced hepatic steatosis in C57BL/6 mice by targeting the LKB1/AMPK pathway; to investigate whether other mechanisms are involved APE induction of improved steatosis, especially roles bile acid (BA) metabolism and gut microbiota, we conducted this study. Thirty-three male were fed with diet for 12 weeks concomitantly treated sterilized water (CON) or 125 500 mg/(kg·bw·day) (low-dose APE, LAP; high-dose...
Abstract Autophagy defection contributes to inflammation dysregulation, which plays an important role in gastric cancer (GC) progression. Various studies have demonstrated that long noncoding RNA could function as novel regulators of autophagy. Previously, MALAT1 was reported upregulated GC cells and positively regulate autophagy various cancers. Here, we for the first time found promote interleukin-6 (IL-6) secretion by blocking autophagic flux. Moreover, IL-6 induced activate normal...
Ovarian cancer is highly lethal and has a poor prognosis due to metastasis. Long non-coding RNAs (lncRNAs) are key regulators of tumor development, but their role in ovarian metastasis remains unclear.The expression lnc-CTSLP8 was analyzed public databases (TCGA GEO) validated via qRT-PCR. Lnc-CTSLP8 overexpression knockout cell lines were constructed using lentiviral vector the CRISP/Cas9 system. Cell proliferation, colony formation, migration, invasion analyzed. An orthotopic mouse model...
To screen samples from patients with presumed autosomal dominant retinitis pigmentosa (adRP) for mutations in 12 disease genes as a contribution to the research and treatment goals of National Ophthalmic Disease Genotyping Phenotyping Network (eyeGENE).DNA were obtained eyeGENE. A total 170 probands an intake diagnosis adRP tested through enrollment The 10 most common causing (IMPDH1, KLHL7, NR2E3, PRPF3/RP18, PRPF31/RP11, PRPF8/RP13, PRPH2/RDS, RHO, RP1, TOPORS) chosen PCR-based dideoxy...