A5100408817- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Pluripotent Stem Cells Research
- Pain Mechanisms and Treatments
- Wound Healing and Treatments
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Asthma and respiratory diseases
- Pediatric Hepatobiliary Diseases and Treatments
- Erythrocyte Function and Pathophysiology
- Dermatologic Treatments and Research
- Microfluidic and Capillary Electrophoresis Applications
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Thermal properties of materials
- Immune Response and Inflammation
- Microfluidic and Bio-sensing Technologies
- Complement system in diseases
- Ion channel regulation and function
- Facial Rejuvenation and Surgery Techniques
- 3D Printing in Biomedical Research
- Blood groups and transfusion
Northeast Agricultural University
2023-2025
Beijing Jiaotong University
1999-2025
Harvard University
2004-2025
Mindray (China)
2025
Harbin University of Science and Technology
2025
Beth Israel Deaconess Medical Center
2007-2025
Lanzhou University
2024
Guangdong Provincial People's Hospital
2009-2024
Ruijin Hospital
2008-2024
Shanghai Jiao Tong University
2024
The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how looping at locus is regulated remains poorly understood. Here we demonstrate a long noncoding RNA (lncRNA), CCAT1-L, transcribed specifically in colorectal cancers from 515 kb upstream of MYC. This lncRNA plays role transcriptional regulation and promotes looping. Importantly, CCAT1-L located within strong super-enhancer spatially close to Knockdown...
A cardinal feature of inflammation is heightened pain sensitivity at the site inflamed tissue. This results from local release by immune and injured cells nociceptor sensitizers, including prostaglandin E 2 , bradykinin, nerve growth factor, that reduce threshold increase excitability peripheral terminals nociceptors so they now respond to innocuous stimuli: phenomenon sensitization. We show here proinflammatory cytokine interleukin-1β (IL-1β), in addition producing inducing synthesis...
Molecular mechanisms underlying C-fiber stimulation-induced ERK (extracellular signal-regulated kinase) activation in dorsal horn neurons and its contribution to central sensitization have been investigated. In adult rat spinal slice preparations, of primary afferents by a brief exposure capsaicin produces an eightfold 10-fold increase phosphorylation (pERK) superficial neurons. The pERK induction is reduced blockade NMDA, AMPA/kainate, group I metabotropic glutamate receptor, neurokinin-1,...
Significance In the current study, we show that brown adipose tissue (BAT) activity is dramatically reduced in a dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) rat when compared with normal control rat. Importantly, key features of PCOS (such as insulin resistance and irregular estrous cycle) are alleviated after BAT transplantation. Mechanistically, transplanted enhances endogenous thereby increases circulating adiponectin level, which was lower both patient model....
We used a mouse with deletion of exons 4, 5, and 6 the SCN11A (sodium channel, voltage-gated, type XI, α) gene that encodes voltage-gated sodium channel Na v 1.9 to assess its contribution pain. is present in nociceptor sensory neurons express TRPV1, bradykinin B 2 , purinergic P2X 3 receptors. In −/− mice, non-inactivating persistent tetrodotoxin-resistant TTXr-Per current absent, whereas TTXr-Slow unchanged. TTXs currents are unaffected by mutation 1.9. Pain hypersensitivity elicited...
Transplantation of embryonic stem cells (ESCs) can improve cardiac function in treatment myocardial infarction. The low rate cell retention and survival within the ischemic tissues makes application transplantation techniques difficult. In this study, we used a temperature-responsive chitosan hydrogel (as scaffold) combined with ESCs to maintain viable infarcted tissue. Temperature-responsive was prepared injected into heart wall rat infarction models alone or together mouse ESCs. result...
Abstract The capacity of airway eosinophils, potentially pertinent to allergic diseases the upper and lower airways, function as professional APCs, those specifically able elicit responses from unprimed, Ag-naive CD4+ T cells has been uncertain. We investigated whether eosinophils are capable initiating naive cell in vivo. Eosinophils, isolated free other APCs spleens IL-5 transgenic mice, following culture with GM-CSF expressed MHC class II costimulatory proteins, CD40, CD80, CD86....
Abstract Background To determine the efficacy and safety of umbilical cord-derived mesenchymal stem cells (UC-MSCs) in Chinese adults with type 2 diabetes mellitus (T2DM). Methods In this single-center, double-blinded, randomized, placebo-controlled phase II trial, 91 patients were randomly assigned to receive intravenous infusion UC-MSCs ( n = 45) or placebo 46) three times 4-week intervals followed up for 48 weeks from October 2015 December 2018. The primary endpoint was percentage...
Acute myocardial infarction (AMI) is a leading cause of mortality worldwide. Adipose-derived stem cell (ADSC) transplantation presents promising therapeutic approach for AMI; however, the harsh microenvironment infarcted myocardium, characterized by hypoxia and oxidative stress, limits survival efficacy ADSCs. Nanozymes (NZs), which have robust anti-oxidative enzyme-mimicking activities, demonstrated potential in combating stress improving viability. Mn3O4 NZs (Mn-Nzs), nanoflower-like...
Bradykinin, an inflammatory mediator, sensitizes nociceptor peripheral terminals reducing pain threshold. We now show that the B 2 kinin receptor is expressed in rat dorsal horn neurons and bradykinin, a -specific agonist, augments AMPA- NMDA-induced, primary afferent-evoked EPSCs, increases frequency amplitude of miniature EPSCs superficial vitro . Administration bradykinin to spinal cord vivo produces, moreover, NMDA-dependent hyperalgesia. also demonstrate nociceptive inputs result...
Abstract Alum, aluminum-hydroxide-containing compounds, long used as adjuvants in human vaccinations, functions by ill-defined, immunostimulatory mechanisms. Antigen-free alum has been shown to act via a previously unidentified, splenic Gr1+, IL-4-expressing myeloid cell population stimulate early B priming. We demonstrate that the alum-elicited and -activated cells are eosinophils function prime responses. Eosinophils principal IL-4+ spleens 6 days following i.p. administration....
Bradykinin potentiates synaptic glutamate release and action in the spinal cord via presynaptic postsynaptic B 2 receptors, contributing thereby to activity-dependent central sensitization pain hypersensitivity (Wang et al., 2005). We have now examined signaling pathways that are responsible for modulatory actions of bradykinin on glutamatergic transmission superficial dorsal horn neurons. receptors coexpressed neurons with protein kinase A (PKA) δ isoform C (PKC), we find augmentation by...
The poor survival rate of transplanted stem cells in ischemic myocardium has limited their therapeutic efficacy. Curcumin potent antioxidant property. This study investigates whether prior curcumin treatment protects from oxidative stress injury and improves myocardial recovery following transplantation. Autologous Sprague-Dawley rat adipose derived mesenchymal (ADSCs) were pretreated with or without curcumin. hydrogen peroxide/serum deprivation (H2O2/SD) medium was used to mimic the...
Abstract The mammalian cell culture process is a key step in commercial therapeutic protein production and needs to be monitored controlled due its complexity. Raman spectroscopy has been reported for monitoring by analysis of many important parameters. However, studies on in‐line the were mainly conducted small or pilot scale. Developing analytical methods commercial‐scale more challenging. In this study, an method was developed glucose, lactate, viable density (VCD) Chinese hamster ovary...