A5100615983- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Aquaculture disease management and microbiota
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic and Kidney Cyst Diseases
- Asthma and respiratory diseases
- Wnt/β-catenin signaling in development and cancer
- Eosinophilic Esophagitis
- Autophagy in Disease and Therapy
- Cardiac Fibrosis and Remodeling
- Dermatology and Skin Diseases
- Biomedical Research and Pathophysiology
- Fungal Biology and Applications
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Renal and related cancers
- Gout, Hyperuricemia, Uric Acid
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Barrier Structure and Function Studies
- Blood Coagulation and Thrombosis Mechanisms
- Aquaculture Nutrition and Growth
- Endoplasmic Reticulum Stress and Disease
- Cancer, Stress, Anesthesia, and Immune Response
Anhui Medical University
2021-2025
Academy of Military Medical Sciences
2025
Hangzhou Medical College
2024
Zhejiang Provincial People's Hospital
2024
Xi'an Jiaotong University
2024
Nanjing Medical University
2008-2023
Peking University
2009-2023
Hebei Medical University
2023
Second Hospital of Hebei Medical University
2023
University of Electronic Science and Technology of China
2012-2021
Rationale: Renal fibrosis is the terminal manifestation of chronic and irreversible renal disease.Effective therapies other than dialysis are extremely limited.In this study, we investigated potential effects targeting elevated interleukin-6 (IL-6) levels in treatment fibrosis.Methods: Fc-gp130 was used to specifically block IL-6 trans-signaling.Unilateral ureteral occlusion (UUO) ischemia reperfusion (IR) mouse models were constructed investigate therapeutic effect on fibrosis.The role...
Rationale: Multiple sclerosis (MS) and its mouse model, experimental autoimmune encephalomyelitis (EAE), are inflammatory disorders of the central nervous system (CNS). The function platelets in pathologies is thus far poorly defined. Objective: We addressed role mediating CNS inflammation EAE. Methods Results: found that were present human MS lesions as well mice subjected to EAE but not from control nondiseased mice. Platelet depletion at effector-inflammatory phase resulted significantly...
To study the mechanisms involved in leukocyte recruitment induced by local bacterial infection within CNS, we used intravital microscopy to visualize interaction between leukocytes and microvasculature brain. First, showed that intracerebroventricular injection of LPS could cause significant rolling adhesion brain postcapillary venules wild-type mice, while negligible was observed TLR4-deficient C57BL/10ScCr mice CD14 knockout suggesting is mediated TLR4/CD14-bearing cells. Moreover, reduced...
Brain inflammation is a frequent consequence of sepsis and septic shock. We imaged leukocyte recruitment in brain postcapillary venules induced by i.p. administration LPS as simple model systemic inflammation. The injection (0.5 mg/kg) significant rolling adhesion wild-type (WT) mice more than 90% were neutrophils. However, no emigrated neutrophils detected parenchyma. High levels TNF-alpha IL-1beta the plasma after but different profile (IL-1beta not TNF-alpha) was brain. caused TLR4...
Recognition of LPS by TLR4 on immune sentinel cells such as macrophages is thought to be key the recruitment neutrophils sites infection with Gram-negative bacteria. To explore whether endothelial plays a role in this process, we engineered and imaged mice that expressed exclusively endothelium (known herein EndotheliumTLR4 mice). Local administration into tissue induced comparable neutrophil wild-type mice. Following systemic or intraperitoneal E. coli administration, most were sequestered...
Epigallocatechin-3-gallate (EGCG) has been demonstrated to inhibit cancer in experimental studies through its antioxidant activity and modulations on cellular functions by binding specific proteins. We previously that EGCG upregulates the expression of microRNA (i.e. miR-210) HIF-1α, resulting reduced cell proliferation anchorage-independent growth. However, affinities HIF-1α many other targets are higher than plasma peak level animals administered with high dose EGCG, raising a concern...
The complement system is becoming increasingly recognized as a key participant in many neurodegenerative diseases of the brain. Complement-deficient animals exhibit reduced neuroinflammation.In present study, we administered intracerebroventricularly lipopolysaccharide (LPS) to mimic local infection brain and investigated role component C3 vasculature endothelial activation leukocyte recruitment. degree neutrophil infiltration was determined by esterase staining. Leukocyte-endothelial...
Objective: Gout, induced by monosodium urate (MSU) crystal deposition in joint tissues, provokes severe pain and impacts life quality of patients. However, the mechanisms underlying gout are still incompletely understood. Methods: We established a mouse model intra-articularly injection MSU crystals into ankle wild type genetic knockout mice. RNA-Sequencing, vivo molecular imaging, Ca2+ reactive oxygen species (ROS) generation, neutrophil influx nocifensive behavioral assays, etc. were used....
Microbial dysbiosis in the skin has been implicated pathogenesis of atopic dermatitis (AD); however, whether and how changes microbiome initiate inflammation, or vice versa, remains poorly understood. Here, we report that levels sebum its microbial metabolite, propionate, were lower on surface AD patients compared with those healthy individuals. Topical propionate application attenuated inflammation mice MC903-induced AD-like by inhibiting IL-33 production keratinocytes, an effect was...
Acute myocardial infarction (AMI) is a leading cause of mortality worldwide. Adipose-derived stem cell (ADSC) transplantation presents promising therapeutic approach for AMI; however, the harsh microenvironment infarcted myocardium, characterized by hypoxia and oxidative stress, limits survival efficacy ADSCs. Nanozymes (NZs), which have robust anti-oxidative enzyme-mimicking activities, demonstrated potential in combating stress improving viability. Mn3O4 NZs (Mn-Nzs), nanoflower-like...
The expression of hepatocyte growth factor (HGF) receptor, encoded by the Met oncogene, is elevated in ovarian and a variety cancers. Here we show that human cancer cells with high were more sensitive to cell motility invasion effect HGF. down-regulation small interfering RNAs or K252a resulted reduced migration response invasive/migratory phenotype activated HGF can be blocked specific inhibitors phosphatidylinositol-3-kinase (PI3K) cascade, inhibitor p70S6K, also dominant-negative Akt,...
Abstract p70 S6 kinase (p70S6K) is a downstream effector of phosphatidylinositol 3-kinase and frequently activated in human ovarian cancer. Here we show that p70S6K functions epithelial to mesenchymal transition (EMT) responsible for the acquisition invasiveness during tumor progression. This tumorigenic activity associated with ability repress E-cadherin through up-regulation Snail. activation induced phenotypic changes consistent EMT cancer cells: The cells lost cell morphology, acquired...
Interleukin-33 (IL-33) is increasingly being recognized as a key immunomodulatory cytokine in many neurological diseases.In the present study, wild-type (WT) and IL-33-/- mice received intracerebroventricular (i.c.v.) injection of lipopolysaccharide (LPS) to induce neuroinflammation. Intravital microscopy was employed examine leukocyte-endothelial interactions brain vasculature. The degree neutrophil infiltration determined by myeloperoxidase (MPO) staining. Real-time PCR western blotting...
Abstract Interleukin‐33 (IL‐33) and its receptor ST2 contribute to spinal glial activation chronic pain. A recent study showed that peripheral IL‐33 plays a pivotal role in the pathogenesis of itch induced by poison ivy. However, how IL‐33/ST2 signaling cord potentially mediates remains elusive. Here, we determined St2 −/− substantially reduced scratching behaviors 2,4‐dinitrofluorobenzene (DNFB)‐induced allergic contact dermatitis (ACD) as well acetone diethylether followed water‐induced...
Hyperuricemia (HUA)-induced oxidative stress is a crucial contributor to hyperuricemic nephropathy (HN), but the molecular mechanisms underlying disturbed redox homeostasis in kidneys remain elusive. Using RNA sequencing, together with biochemical analyses, we found that nuclear factor erythroid 2-related 2 (NRF2) expression and localization levels were increased early HN progression then gradually declined below baseline level. We identified impaired activity of NRF2-activated antioxidant...
The nervous system is constantly infiltrated by blood-derived sentinels known as perivascular macrophages. Their immediate precursors have not yet been identified in situ and the mechanism that governs their recruitment mostly unknown. Here, we provide evidence CD68 + GR1 − monocytes can give rise to macrophages mice suffering from endotoxemia. After adhesion endothelium, these start crawl, adopt a rod-shaped morphology when passing through capillaries, manifest ability proliferate form long...
Renal ischemia reperfusion injury (RIRI) is one of the main causes acute kidney (AKI), which can lead to renal failure. The development RIRI so complicated that it involves many factors such as inflammatory response, oxidative stress and cell apoptosis. Ganoderic acids (GAs), pharmacological components Ganoderma lucidum, have been reported possess anti-inflammatory, antioxidant, other effects. study aimed investigate protective effect GAs on explore related underlying mechanisms. mechanisms...
Background: The cAMP-PKA signaling pathway and TGF-β1-dependent fibrosis pathways are of particular importance in ADPKD progression, but the cross-talk between these remains unclear. Therefore, we used an MDCK-cell model embryonic kidney-cyst to study regulatory role TGF-β1 induced fibrotic process. Method Results: Pkd1flox/flox; Ksp-Cre Pkd1+/+; mice were as vivo model. Increased kidney volume, renal cysts formation up-regulation fibrosis-related proteins TGF-β1, connective tissue growth...
Abstract Tristetraprolin (TTP) is an adenine/uridine (AU)-rich element (ARE)-binding protein that can induce degradation of mRNAs. In this study, we report TTP suppresses the expression interleukin-33 (IL-33), a tumor-promoting inflammatory cytokine and thereby inhibits progression gastric cancer (GC). Overexpression decreased level IL-33, whereas knockdown increased IL-33 levels. We also discovered inhibited proliferation, migration invasion GC cell lines through regulation IL-33....