A5010530574- Genetics, Aging, and Longevity in Model Organisms
- Ferroptosis and cancer prognosis
- Gene Regulatory Network Analysis
- Fungal and yeast genetics research
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- Telomeres, Telomerase, and Senescence
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Bioinformatics and Genomic Networks
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Computational Drug Discovery Methods
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Diet and metabolism studies
- Sirtuins and Resveratrol in Medicine
- CRISPR and Genetic Engineering
- Traditional Chinese Medicine Analysis
- Single-cell and spatial transcriptomics
- Biofuel production and bioconversion
- Dietary Effects on Health
- Metabolism, Diabetes, and Cancer
- Caveolin-1 and cellular processes
- Heterotopic Ossification and Related Conditions
Peking University
2015-2025
Peking University International Hospital
2020-2025
State Key Laboratory of Natural and Biomimetic Drugs
2025
Jiangsu University
2014-2015
University of California, San Francisco
2012-2015
QB3
2012-2015
Center for Life Sciences
2015
Resistance to anoikis is a key characteristic of many cancer cells, promoting cell survival. However, the mechanism in hepatocellular carcinoma (HCC) remains unknown. In this study, we applied differentially expressed overlapping anoikis-related genes classify The Cancer Genome Atlas (TCGA) samples using an unsupervised cluster algorithm. Then, employed weighted gene coexpression network analysis (WGCNA) identify highly correlated and constructed prognostic risk model based on univariate Cox...
Budding yeast has served as an important model organism for aging research, and previous genetic studies have led to the discovery of conserved genes/pathways that regulate lifespan across species. However, molecular causes death remain elusive, because it is very difficult directly observe cellular events accompanying in single cells by traditional approach based on micromanipulation. We developed a microfluidic system track individual mother throughout their lifespan, allowing automated...
Significance In this work, we took a proteome-centric view to analyze the cell division and lifespan asymmetry between mother daughter cells in budding yeast. Using flow cytometry-based, high-throughput approach, quantified partitioning of proteome identified 74 mother-enriched 60 daughter-enriched proteins. Functional analysis these proteins suggests mechanisms asymmetric at an organelle/suborganelle level. We found that are much more likely becoming aging factors than those chosen random....
Although aging and senescence have been extensively studied in the past few decades, however, there is lack of clinical treatment available for anti-aging. This study presents effects berberine (BBR) on process resulting a promising extension lifespan model organisms. BBR extended replicative lifespan, improved morphology, boosted rejuvenation markers human fetal lung diploid fibroblasts (2BS WI38). also rescued senescent cells with late population doubling (PD). Furthermore,...
The primate frontal lobe (FL) is sensitive to aging-related neurocognitive decline. However, the aging-associated molecular mechanisms remain unclear. Here, using physiologically aged non-human primates (NHPs), we depicted a comprehensive landscape of FL aging with multidimensional profiling encompassing bulk and single-nucleus transcriptomes, quantitative proteome, DNA methylome. Conjoint analysis across these neuropathological layers underscores nuclear lamina heterochromatin erosion,...
A major limitation to yeast aging study has been the inability track mother cells and observe molecular markers during process. The traditional lifespan assay relies on manual micro-manipulation remove daughter from mother, which is laborious, time consuming, does not allow long term tracking with high resolution microscopy. Recently, we have developed a microfluidic system capable of retaining in chambers while removing automatically, making it possible fluorescent reporters single...
Background/Aims: Non-alcoholic fatty liver disease (NAFLD) encompasses a series of pathologic changes ranging from steatosis to steatohepatitis, which may progress cirrhosis and hepatocellular carcinoma. The purpose this study was determine whether ganoderma lucidum polysaccharide peptide (GLPP) has therapeutic effect on NAFLD. Methods: Ob/ ob mouse model ApoC3 transgenic were used for exploring the GLPP Key metabolic pathways enzymes identified by metabolomics combining with KEGG PIUmet...
Lung squamous cell carcinoma (LUSC) is often diagnosed at the advanced stage with poor prognosis. The mechanisms of its pathogenesis and prognosis require urgent elucidation. This study was performed to screen potential biomarkers related occurrence, development LUSC reveal unknown physiological pathological processes. Using bioinformatics analysis, lung microarray datasets from Gene Expression Omnibus (GEO) Cancer Genome Atlas (TCGA) databases were analyzed identify differentially expressed...
Venous malformations (VMs) represent prevalent vascular anomalies typically attributed to non-inherited somatic mutations within venous endothelial cells (VECs). The lack of robust disease models for VMs impedes drug discovery. Here, we devise a protocol the generation human induced VECs (iVECs) through manipulation cell-cycle dynamics via retinoic signaling pathway. We introduce an L914F mutation into TIE2 gene locus pluripotent stem (iPSCs) and show that mutated iVECs form dilated blood...
Abstract Histone acetyltransferases (HATs) are important enzymes that transfer acetyl groups onto histones and thereby regulate both gene expression chromosomal structures. Previous work has shown the activation of sirtuins, which histone deacetylases, can extend lifespan. This suggests inhibiting HATs may have a similar beneficial effect. In present study, we utilized range HAT inhibitors or heterozygous Gcn5 Ngg1 mutants to demonstrate marked yeast life extension. human cell lines,...
Aging is a major risk factor for chronic diseases and disability in humans. Nowadays, no effective anti-aging treatment available clinically. In this study, oridonin was selected based on the drug screening strategy similar to Connectivity MAP (CMAP) but upon transcriptomes of 102 traditional Chinese medicines treated cell lines. Oridonin diterpenoid isolated from Rabdosia rubescens. As reported, exhibits variety pharmacological activities, including antitumor, antibacterial...
Maintaining the stemness of bone marrow mesenchymal stem cells (BMMSCs) is crucial for homeostasis and regeneration. However, in vitro expansion diseases impair BMMSC stemness, limiting its functionality tissue engineering. Using a deep learning-based efficacy prediction system sequencing, we identify natural small-molecule compound, dihydroartemisinin (DHA), that maintains enhances During long-term expansion, DHA preserves characteristics, including self-renewal ability unbiased...
Abstract The pandemic COVID-19 has spread to all over the world and greatly threatens safety health of people. is highly infectious with high mortality rate. As no effective antiviral treatment currently available, new drugs are urgently needed. We employed transcriptional analysis uncover potential from natural products or FDA approved drugs. found liquiritin significantly inhibit replication SARS-CoV-2 in Vero E6 cells EC 50 = 2.39 μM. Mechanistically, we exerts anti-viral function by...
Ferroptosis is a new type of programmed cell death, which occurs with iron dependence. Previous studies have showed that ferroptosis plays an important regulatory role in the occurrence and development tumors. Colon cancer one major morbidities causes mortality world. This study used RNA-seq colon clinical data to explore relationship between ferroptosis-related genes cancer. Based on fifteen prognostic genes, two molecular subgroups were identified. Surprisingly, we also found cluster2 was...
Aging is a significant risk factor for chronic diseases and disability, yet effective anti-aging interventions remain elusive. We explored the potential of 3-hydroxybutyrate (3HB), an endogenous metabolite with established safety, to modulate longevity in mice. In this study, we employed 2BS WI-38 cell models, yeast model, naturally aging mouse models investigate effects 3HB on various systems. Additionally, utilized RNA sequencing metabolomics technologies explore mechanisms underlying...
Adult tendon stem/progenitor cells (TSPCs) are essential for maintenance, regeneration, and repair, yet they become susceptible to senescence with age, impairing the self-healing capacity of tendons. In this study, we employ a recently developed deep-learning-based efficacy prediction system screen potential stemness-promoting senescence-inhibiting drugs from natural products using transcriptional signatures stemness. The top-ranked candidate, prim-O-glucosylcimifugin (POG), saposhnikovia...
Although the NaV1.7 sodium channel is a promising drug target for pain, traditional screening strategies discovery of inhibitors are very painstaking and time-consuming. Herein, we aimed to build machine learning models design potent effective inhibitors. We customized imbalanced data set from ChEMBL BindingDB train filter best classification model. Then, whole-cell voltage-clamp was employed validate assembled molecular group optimization method by combining Grammar Variational Autoencoder,...
In the era of synthetic biology, design, construction, and utilization chromosomes with unique features provide a strategy to study complex cellular processes such as aging. Herein, we successfully construct 884 Kb synXIII Saccharomyces cerevisiae investigate replicative aging using these strains. We verify that up-regulation rRNA-related transcriptional factor, RRN9, positively influence lifespan. Using SCRaMbLE system enables inducible whole-genome rearrangement on synXIII, obtain 20...