A5065277510- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Extracellular vesicles in disease
- Acute Kidney Injury Research
- Proteins in Food Systems
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- MicroRNA in disease regulation
- Crystallization and Solubility Studies
- Cardiac Ischemia and Reperfusion
- Pickering emulsions and particle stabilization
- RNA Interference and Gene Delivery
- Tryptophan and brain disorders
- Drug-Induced Hepatotoxicity and Protection
- Metabolomics and Mass Spectrometry Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Gout, Hyperuricemia, Uric Acid
- Polysaccharides and Plant Cell Walls
- Neuroscience and Neural Engineering
- Immune Cell Function and Interaction
- Electrospun Nanofibers in Biomedical Applications
- Polysaccharides Composition and Applications
- Organ Transplantation Techniques and Outcomes
- Natural Compounds in Disease Treatment
Shandong Academy of Sciences
2021-2025
Qilu University of Technology
2021-2025
Sichuan University
2017-2025
West China Hospital of Sichuan University
2017-2025
Chinese Center For Disease Control and Prevention
2025
Harbin Medical University
2025
Guizhou Provincial People's Hospital
2025
Northwest Normal University
2024
Jiangxi Agricultural University
2019-2024
State Key Laboratory of Surface Physics
2022-2024
Aims: Ischemia-reperfusion injury (IRI)-induced acute kidney (IRI-AKI) is characterized by elevated levels of reactive oxygen species (ROS), mitochondrial dysfunction, and inflammation, but the potential link among these features remains unclear. In this study, we aimed to investigate specific role ROS (mtROS) in initiating DNA (mtDNA) damage inflammation during IRI-AKI. Methods: The changes renal function, IRI-AKI mice with or without mtROS inhibition were analyzed vivo. impact on TFAM...
Mitochondrial dysfunction is a key feature of injury to numerous tissues and stem cell aging. Although the tissue regenerative role mesenchymal (MSC)-derived extracellular vesicles (MSC-EVs) well known, their specific in regulating mitochondrial function target cells remains elusive. Here, we report that MSC-EVs attenuated mtDNA damage inflammation after acute kidney (AKI) this effect was at least partially dependent on transcription factor A (TFAM) pathway. In detail, TFAM were depleted by...
Rodent hearts can regenerate myocardium lost to apical resection or myocardial infarction for up 7 days after birth, but whether a similar window regeneration also exists in large mammals is unknown.Acute (AMI) was surgically induced neonatal pigs on postnatal 1, 2, 3, 7, and 14 (ie, the P1, P2, P3, P7, P14 groups, respectively). Cardiac systolic function evaluated before AMI at 30 post-AMI via transthoracic echocardiography. Cardiomyocyte cell cycle activity assessed immunostaining...
Rationale: The effectiveness of transplanted, human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) for treatment ischemic myocardial injury is limited by the exceptionally low engraftment rate. Objective: To determine whether overexpression cell cycle activator CCND2 (cyclin D2) in hiPSC-CMs can increase graft size and improve recovery a mouse model infarction increasing proliferation grafted cells. Methods Results: Human was delivered to hiPSCs via lentiviral-mediated gene...
Vascular endothelial growth factor (VEGF) is a well-characterized proangiogenic cytokine that has been shown to promote neovascularization in hearts of patients with ischemic heart disease but can also lead adverse effects depending on the dose and mode delivery. We investigated whether prolonged exposure low VEGF could be achieved by encapsulating polylactic coglycolic acid nanoparticles treatment VEGF-containing improved cardiac function protected against left ventricular remodeling mice...
Background: Human induced pluripotent stem cells with normal (wild-type) or upregulated (overexpressed) levels of CCND2 (cyclin D2) expression were differentiated into cardiomyocytes (CCND2 WT CMs OE CMs, respectively) and injected infarcted pig hearts. Methods: Acute myocardial infarction was by a 60-minute occlusion the left anterior descending coronary artery. Immediately after reperfusion, (3×10 7 each) an equivalent volume delivery vehicle around infarct border zone area. Results: The...
Abstract Differentiation of cardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) is critically dependent upon the regulation Wnt signaling pathway. The mechanisms remain unclear with regard to dose and timing each differentiation inducer, interaction inducers that regulate in mesendoderm specification cardiac mesoderm. Consequently, it remains far optimal efficiency consistency hiPSC lines CMs. Here, we have carefully deciphered role pathway manipulation on mesoderm a...
Cytokine immunotherapy represents an attractive strategy to stimulate robust immune responses for renal injury repair in ischemic acute kidney (AKI). However, its clinical application is hindered by nonspecificity kidney, short circulation half-life, and severe side effects. An ideal cytokine AKI requires preferential delivery of cytokines with accurate dosage the sustained-release responses. Herein, we developed a DNA nanoraft precisely arranging interleukin-33 (IL-33) nanoarray on...
Abstract Hyperuricemia is an important risk factor for cardiovascular and renal diseases. Phloretin had shown antioxidant anti‐inflammatory properties, but its role in endothelial injury rarely reported. In this study, we aimed to investigate the protective effect of phloretin on UA ‐induced human umbilical vein cells. The effects cell viability, inflammation, THP ‐1 monocyte adhesion, tube formation, GLUT 9 expression uptake cells were evaluated. changes nuclear factor‐kappa B/extracellular...
Abstract Acute kidney injury (AKI) is a clinical condition that associated with high morbidity and mortality. Inflammation reported to play key role in AKI. Although the M2 macrophages exhibit antimicrobial anti‐inflammatory activities, their therapeutic potential has not been evaluated for This study aimed investigate protective effect of peritoneal macrophage transplantation on AKI mice. The were isolated from dialysates induced undergo polarization using interleukin (IL)‐4/IL‐13. was mice...
Abstract Diabetic kidney disease (DKD) is a severe DM complication. While complement C5 up‐regulation and gut dysbiosis are found in T2DM, their roles DKD unclear. Here, we investigated the effect of on microbiota during development. Renal C5a/C5a receptor (C5aR) expression changes were measured T2DM patients db/db mice. Db/db mice treated with C5aR antagonist (C5aRA), renal function, genome analysed. The effects C5a short‐chain fatty acids (SCFAs) signal transducer activator transcription 3...
Persistent mitochondrial injury occurs after acute kidney (AKI) and mitochondria-targeted antioxidant Mito-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) (MT) has shown benefits for AKI, but its efficiency is limited by short half-life side effect in vivo. Self-assembling peptide (SAP) hydrogel a robust platform drug delivery. This study aims to develop an SAP-based carrier slow release MT enhancing long-term therapeutic potency on AKI. The KLD with aspartic acid (KLDD) was designed....