A5100351924- Cardiac electrophysiology and arrhythmias
- Cardiac Valve Diseases and Treatments
- Inflammasome and immune disorders
- Ion channel regulation and function
- Sphingolipid Metabolism and Signaling
- Infective Endocarditis Diagnosis and Management
- Cardiovascular Function and Risk Factors
- Angiogenesis and VEGF in Cancer
- Atrial Fibrillation Management and Outcomes
- Cardiac Fibrosis and Remodeling
- Acute Myocardial Infarction Research
- Kruppel-like factors research
- Cardiac Structural Anomalies and Repair
- Cardiac Arrhythmias and Treatments
- Heart Failure Treatment and Management
- Cardiac Imaging and Diagnostics
- Cardiovascular Effects of Exercise
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Signaling Pathways in Disease
- Connective Tissue Growth Factor Research
- Cardiovascular Disease and Adiposity
- Nitric Oxide and Endothelin Effects
- Blood Pressure and Hypertension Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Shanghai East Hospital
2016-2025
State Key Laboratory of Cardiovascular Disease
2024-2025
Gansu University of Traditional Chinese Medicine
2025
Tongji University
2013-2024
Monash University
2024
Suzhou Research Institute
2023-2024
Qingdao University
2021-2024
Affiliated Hospital of Qingdao University
2021-2024
Chinese PLA General Hospital
2010-2024
Wannan Medical College
2023-2024
The cardiac ryanodine receptor (RyR2) governs the release of Ca2+ from sarcoplasmic reticulum, which initiates muscle contraction. Mutations in RyR2 have been linked to ventricular tachycardia (VT) and sudden death, but precise molecular mechanism is unclear. It known that when reticulum store content reaches a critical level, spontaneous occurs, process we refer as store-overload-induced (SOICR). In view well documented arrhythmogenic nature SOICR, characterized effects disease-causing...
Ventricular tachycardia (VT) is the leading cause of sudden death, and cardiac ryanodine receptor (RyR2) emerging as an important focus in its pathogenesis. RyR2 mutations have been linked to VT but their precise impacts on channel function remain largely undefined controversial. We previously shown that several disease-linked C-terminal region enhance sensitivity activation by luminal Ca2+. Cells expressing these mutants display increased propensity for spontaneous Ca2+ release under...
Rationale: Endothelial dysfunction results in sustained and chronic vascular inflammation, which is central to atherosclerotic diseases. However, transcriptional regulation of endothelial inflammation has not been well clarified. Objective: This study aims explore Foxp (forkhead box P) transcription factor 1 homeostasis, atherogenesis, its mechanisms. Methods Results: To assess the importance Foxp1 atherosclerosis, expression was analyzed human coronary artery mouse artery, we observed...
Members of the CCN matricellular protein family are crucial in various biological processes. This study aimed to characterize vascular cell-specific effects CCN5 on neointimal formation and its role preventing in-stent restenosis (ISR) after percutaneous coronary intervention (PCI). Stent-implanted porcine artery RNA-seq mouse injury-induced femoral neointima single-cell RNA sequencing were performed. Plasma levels measured by enzyme-linked immunosorbent assay. Endothelial cell (EC)- smooth...
Millions of platelets are produced each hour by bone marrow (BM) megakaryocytes (MKs). MKs extend transendothelial proplatelet (PP) extensions into BM sinusoids and shed new the blood. The mechanisms that control platelet generation remain incompletely understood. Using conditional mutants intravital multiphoton microscopy, we show here lipid mediator sphingosine 1-phosphate (S1P) serves as a critical directional cue guiding elongation megakaryocytic PP from interstitium triggering...
Due to its unique location, the endothelial surface glycocalyx (ESG) at luminal side of microvessel wall may serve as a mechano-sensor and transducer blood flow thus regulate functions. To examine this role ESG, we used fluorescence microscopy measure nitric oxide (NO) production in post-capillary venules arterioles rat mesentery under reduced (low) normal (high) conditions, with without enzyme pretreatment remove heparan sulfate (HS) ESG presence an synthase (eNOS) inhibitor,...
Objective— Increasing evidence suggests that contractile dysfunction in smooth muscle cells (SMCs) plays a critical role aortic biomechanical and aneurysm dissection (AAD) development. However, the mechanisms underlying SMC sporadic AAD are poorly understood. In this study, we examined of NLRP3 (nucleotide oligomerization domain–like receptor family, pyrin domain containing 3)–caspase-1 inflammasome, key inflammatory cascade, AAD. Approach Results— We observed significant protein degradation...
Background Aortic aneurysms and dissections are highly lethal diseases for which an effective treatment strategy is critically needed to prevent disease progression. The nucleotide‐binding oligomerization domain–like receptor pyrin domain containing 3 (NLRP3)–caspase‐1 inflammasome cascade was recently shown play important role in aortic destruction development. In this study, we tested the effects of MCC950, a potent, selective NLRP3 inhibitor, on preventing aneurysm dissection formation....
Cardiac ryanodine receptor (RyR2) gain-of-function mutations cause catecholaminergic polymorphic ventricular tachycardia, a condition characterized by prominent ectopy in response to catecholamine stress, which can be reproduced on exercise stress testing (EST). However, reports of sudden cardiac death (SCD) have emerged EST-negative individuals who loss-of-function (LOF) RyR2 mutations. The clinical relevance LOF including their pathogenic mechanism, diagnosis, and treatment are all...
Lapatinib (LAP) is an important anti-cancer drug and frequently alongside doxorubicin (DOX) as a combination therapy for better efficacy. However, many studies have reported that LAP in with DOX may induce highly cardiotoxicity. Accordingly, we aimed to explore the potential mechanism involved synergistic effect of DOX-induced Here, cell counting kit-8 was used detect viability lactate dehydrogenase measurement performed assess injury. Cell apoptosis evaluated by TUNEL assay western blot...
Abstract Background: NOD-like receptor family pyrin domain containing 3 (NLRP3)-mediated macrophage pyroptosis plays an important role in sepsis-induced acute lung injury (ALI). Inhibition of may be a way to alleviate inflammation as well tissue damage triggered after lipopolysaccharide (LPS) stimulation. The aim the present study was explore whether buformin (BF), hypoglycemic agent, could ALI by inhibiting pyroptosis. Methods: Wildtype C57BL/6 mice were randomly divided into control group,...
Silica nanoparticle (SiNP) exposure induces severe pulmonary inflammation and fibrosis, but the pathogenesis remains unclear, effective therapies are currently lacking. To explore mechanism underlying SiNPs-induced we constructed in vivo silica animal models vitro of silica-induced macrophage pyroptosis fibroblast transdifferentiation. We found that SiNP elicits upregulation proteins associated with pyroptosis, including NLRP3, ASC, IL-1β, GSDMD, while immunofluorescence staining...
Mutations in the human cardiac Ca 2+ release channel (ryanodine receptor, RyR2) gene have recently been shown to cause effort-induced ventricular arrhythmias. However, consequences of these disease-causing mutations RyR2 function are unknown. In present study, we characterized properties mutation R4496C mouse RyR2, which is equivalent a R4497C, by heterologous expression mutant HEK293 cells. [ 3 H]ryanodine binding studies revealed that resulted an increase activity particular at low...
Different forms of ventricular arrhythmias have been linked to mutations in the cardiac ryanodine receptor (RyR)2, but molecular basis for this phenotypic heterogeneity is unknown. We recently demonstrated that an enhanced sensitivity luminal Ca(2+) and increased propensity spontaneous release or store-overload-induced (SOICR) are common defects RyR2 associated with catecholaminergic polymorphic bidirectional tachycardia. Here, we investigated properties a unique mutation idiopathic...
The 12.6-kDa FK506-binding protein (FKBP12.6) is considered to be a key regulator of the cardiac ryanodine receptor (RyR2), but its precise role in RyR2 function complex and controversial. In present study we investigated impact FKBP12.6 removal on properties channel propensity for spontaneous Ca2+ release occurrence ventricular arrhythmias. Single recordings lipid bilayers showed that FK506 treatment recombinant co-expressed with or without native canine did not induce long-lived...
A reliable prediction tool is needed to identify acute coronary syndrome (ACS) patients with high mortality risk after their initial hospitalization. EPICOR (long-tErm follow uP of antithrombotic management patterns In CORonary patients: NCT01171404) a prospective cohort study 10,568 consecutive hospital survivors an ACS event (4943 ST-segment elevation myocardial infarction (STEMI) and 5625 non-ST-elevation (NSTE-ACS)). Of these cases, 65.1% underwent percutaneous intervention (PCI) 2.5%...
The aim of the study was to evaluate role purinergic receptor P2Y, G protein-coupled 12 (P2Y12), an ADP receptor, in development atherosclerotic lesions.Apolipoprotein E-null mice were crossed with P2y12(-/-) generate double knockout mice. and control apolipoprotein fed a high-fat diet for 20 weeks. Assessment lesions demonstrated that P2Y12 deficiency caused diminished lesion area, increased fibrous content at plaque site, decreased monocyte/macrophage infiltration lesions. Polymerase chain...
Pathological cardiac fibrosis and hypertrophy, the common features of left ventricular remodeling, often progress to heart failure. Forkhead box transcription factor P1 (Foxp1) in endothelial cells (ECs) has been shown play an important role development. However, effect EC-Foxp1 on pathological remodeling not well clarified. This study aims determine underlying mechanisms.Foxp1 EC-specific loss-of-function gain-of-function mice were generated, angiotensin II infusion or a transverse aortic...
Abstract Circular RNA (circRNA) is a key regulator in the development and progression of human cancers. Previous studies confirmed circRNA‐0008717 (circABCB10) as an oncogene osteosarcoma, but regulatory effect circABCB10 nonsmall cell lung cancer (NSCLC) still unclear. In current study, we examined expression different NSCLC lines. Bioinformatics analysis, Cell Counting Kit‐8 assays, Transwell migration, fluorescein reporting experiments, xenografts mice were used to detect on proliferation...
Abstract Objective S1P was found to protect the ESG by inhibiting MMP activity‐dependent shedding of in cultured endothelial cell studies. We aimed further test that contributes maintenance normal vascular permeability protecting intact microvessels. Methods quantified post‐capillary venules rat mesentery and measured albumin presence absence 1 μ M S1P. also inhibitors compared with those predicted a transport model for inter‐endothelial cleft. Results S1P, fluorescence intensity FITC...