A5038627729- Barrier Structure and Function Studies
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Acute Ischemic Stroke Management
- Immune Response and Inflammation
- Congenital Diaphragmatic Hernia Studies
- MicroRNA in disease regulation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Atrial Fibrillation Management and Outcomes
- Blood properties and coagulation
- Microplastics and Plastic Pollution
- Nitric Oxide and Endothelin Effects
- Cancer Cells and Metastasis
- Drug Transport and Resistance Mechanisms
- Erythrocyte Function and Pathophysiology
- Neurological Disease Mechanisms and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Hemoglobin structure and function
- Renin-Angiotensin System Studies
- Pulmonary Hypertension Research and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Congenital Heart Disease Studies
- Antimicrobial Peptides and Activities
- Tracheal and airway disorders
South China Agricultural University
2016-2024
First Affiliated Hospital of GuangXi Medical University
2013-2024
Guangxi Medical University
2013-2024
Hunan Children's Hospital
2024
Affiliated Hospital of Southwest Medical University
2018-2024
Shanghai Jiao Tong University
2024
Shanghai First People's Hospital
2024
Central South University
2024
University of South China
2024
Inner Mongolia University
2008-2023
Methylglyoxal (MGO) is an active metabolite of glucose and plays a prominent role in the pathogenesis diabetic vascular complications, including endothelial cell apoptosis induced by oxidative stress. Metformin (MET), widely prescribed antidiabetic agent, appears to reduce excessive reactive oxygen species (ROS) generation limit apoptosis. However, molecular mechanisms underlying this process are still not fully elucidated. We reported here that MET prevents MGO-induced suppressing stress...
Due to its unique location, the endothelial surface glycocalyx (ESG) at luminal side of microvessel wall may serve as a mechano-sensor and transducer blood flow thus regulate functions. To examine this role ESG, we used fluorescence microscopy measure nitric oxide (NO) production in post-capillary venules arterioles rat mesentery under reduced (low) normal (high) conditions, with without enzyme pretreatment remove heparan sulfate (HS) ESG presence an synthase (eNOS) inhibitor,...
Our previous experiments indicated that GTPases, other than RhoA, are important for the maintenance of endothelial barrier integrity in both intact microvessels rats and mice cultured mouse myocardial (MyEnd) cell monolayers ( J Physiol 539: 295–308, 2002). In present study, we inhibited GTPase Rac by Clostridium sordellii lethal toxin (LT) investigated relation between degree inhibition glucosylation increased permeability. rat venular microvessels, LT (200 ng/ml) hydraulic conductivity...
Development of an optimal systemic drug delivery strategy to the brain will require noninvasive or minimally invasive methods quantify permeability cerebral microvessel wall blood-brain barrier (BBB) various therapeutic agents and measure their transport in tissue. To address this problem, we used laser-scanning multiphoton microscopy determine BBB solutes (P) effective solute diffusion coefficients (Deff) rat tissue 100–250 μm below pia mater. The microcirculation was observed through a...
Abstract Objective S1P was found to protect the ESG by inhibiting MMP activity‐dependent shedding of in cultured endothelial cell studies. We aimed further test that contributes maintenance normal vascular permeability protecting intact microvessels. Methods quantified post‐capillary venules rat mesentery and measured albumin presence absence 1 μ M S1P. also inhibitors compared with those predicted a transport model for inter‐endothelial cleft. Results S1P, fluorescence intensity FITC...
We tested the hypothesis that acutely induced hyperpermeability is dependent on actin-myosin contractility by using individually perfused mesentery venules of pentobarbital-anesthetized rats. Venule hydraulic conductivity (Lp) was measured to monitor response platelet-activating factor (PAF) 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine or bradykinin. Perfusion with PAF (10 nM) a robust transient high Lp [24.3 +/- 1.7 x 10-7 cm/(s.cmH2O)] peaked in 8.9 0.5 min and then returned toward...
We reported previously that increasing cAMP levels in endothelial cells attenuated ATP-induced increases hydraulic conductivity ( L p ), and the activation of cGMP-dependent pathways was a necessary step to increase response inflammatory mediators. The aim present study evaluate role basal microvessel permeability under resting conditions cross talk between cAMP- signaling mechanisms regulation stimulated conditions, using individually perfused microvessels from frog rat mesenteries. found...
Nitric oxide (NO) at different concentrations may promote or inhibit tumor growth and metastasis under various conditions. To test the hypothesis that cells prefer to adhere locations with a higher endothelial NO production in intact microvessels physiological flows further inhibiting decreases cell adhesion, we used intravital fluorescence microscopy measure adhesion postcapillary venules of rat mesentery normal reduced flow conditions, presence an nitric synthase (eNOS) inhibitor,...
To investigate the mechanisms whereby guanosine 3′,5′-cyclic monophosphate (cGMP) modulates microvessel permeability in vivo, we measured changes hydraulic conductivity ( L p ) and endothelial cytoplasmic Ca 2+ concentration ([Ca ] i response to cGMP analogs 8-bromo-cGMP (8-BrcGMP) 8-( p-chlorophenylthio)cGMP (8-pCPT-cGMP) presence absence of inflammatory stimuli intact individually perfused microvessels frog rat mesenteries. The analog caused a transient increase potentiated ATP or...
It is known that Toll-like receptor (TLR)-4 plays an important role in myocardial infarction and atherothrombosis. The of TLR-4 arterial thrombosis undefined. Both TLR-4-deficient (TLR-4(-/-)) wild-type (WT) mice were subjected to FeCl3 carotid artery injury, the time required form occlusive thrombus was measured. mean occlusion TLR-4(-/-) significantly greater than WT after injury (303 ± 32 vs. 165 34 s, P < 0.05). Furthermore, when we used a or TLR-4(-/-)-derived platelet reinfusion...
RT-qPCR has been widely used for gene expression analysis in recent years. The accuracy of this technique largely depends on the selection suitable reference genes. In order to facilitate wild and cultivated Cannabis, stability seven candidate genes (ACT2, 18S rRNA, GAPDH, UBQ, TUB, PP2A EF1α) were assessed leaves samples different development stages organs both Cannabis present study. Their stabilities evaluated through three software packages (GeNorm, Normfinder Bestkeeper). Results showed...
Alcohol oxidase I (AOX1) promoter is the most popular but strictly-regulated methanol inducible for heterologous protein expression in Pichia pastoris. In recent years, AOX1 libraries have been developed with deletion or insertion methods. The present research manipulated poly (dA:dT) tracts this to control strength, which hadn't tried before. There were 34 variants derived from native constructed. And integrated into same genomic location and upstream of reporter gene porcine growth hormone...
To investigate whether vascular endothelial growth factor (VEGF) enhances cancer cell adhesion to normal microvessels, we used in vivo video microscopy measure rates of MDA-MB-435s human breast cells and ErbB2-transformed mouse mammary carcinomas the postcapillary venules rat mesentery. An individual venule mesentery was injected via a glass micropipette with either perfusate mammalian Ringer solution containing 1% bovine serum albumin as control, or addition 1 nm VEGF for test measurements....
Radioimmunotherapy using a radiolabeled monoclonal antibody that targets tumor cells has been shown to be efficient for the treatment of many malignant cancers, with reduced side effects. However, blood-brain barrier (BBB) inhibits transport intravenous antibodies tumors in brain. Recent studies have demonstrated focused ultrasound (FUS) combined microbubbles (MBs) is promising method transiently disrupt BBB drug delivery central nervous system. To find optimal FUS and MBs can induce...
Type 2 diabetic mellitus (DM2) is associated with accelerated thrombotic complications and characterized by high levels of plasminogen activator inhibitor-1 (PAI-1). Recent studies show that human platelets have miR-30c synthesize considerable active PAI-1. The underlying mechanism how PAI-1 expression upregulated in DM2 poorly understood. We now report hyperglycaemia-induced repression increases thrombus formation DM2. Bioinformatic analysis identification miRNA targets were assessed using...