A5029937904- Spectroscopy Techniques in Biomedical and Chemical Research
- Metabolomics and Mass Spectrometry Studies
- Spectroscopy and Chemometric Analyses
- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Orbital Angular Momentum in Optics
- Photoreceptor and optogenetics research
- Advanced Optical Imaging Technologies
- Virtual Reality Applications and Impacts
- Advanced Fiber Laser Technologies
- Mass Spectrometry Techniques and Applications
- Laser-Matter Interactions and Applications
- Optical Coherence Tomography Applications
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Molecular Biology Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Barrier Structure and Function Studies
- Photorefractive and Nonlinear Optics
- Adipose Tissue and Metabolism
- Alzheimer's disease research and treatments
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Advanced Proteomics Techniques and Applications
La Jolla Bioengineering Institute
2019-2025
University of California, San Diego
2019-2025
Ministry of Industry and Information Technology
2025
Wenzhou Medical University
2008-2024
First Affiliated Hospital of Wenzhou Medical University
2008-2024
University of California System
2024
First People's Hospital of Kunshan
2024
Jiangsu University
2024
Columbia University
2016-2022
UC San Diego Health System
2022
Near‐infrared (NIR) radiation has been employed using one‐ and two‐photon excitation of fluorescence imaging at wavelengths 650–950 nm (optical window I) for deep brain imaging; however, longer in NIR have overlooked due to a lack suitable NIR‐low band gap semiconductor detectors and/or femtosecond laser sources. This research introduces three new optical windows demonstrates their potential tissue imaging. The transmittances are measured rat the second (II, 1,100–1,350 nm), third (III,...
Direct visualization of metabolic dynamics in living animals with high spatial and temporal resolution is essential to understanding many biological processes. Here we introduce a platform that combines deuterium oxide (D2O) probing stimulated Raman scattering (DO-SRS) microscopy image situ activities. Enzymatic incorporation D2O-derived into macromolecules generates carbon-deuterium (C-D) bonds, which track biosynthesis tissues can be imaged by SRS situ. Within the broad vibrational spectra...
Abstract A new approach for early detection of multiple cancers is presented by integrating SERS spectroscopy serum molecular fingerprints and machine learning.
Abstract Lipids play crucial roles in many biological processes. Mapping spatial distributions and examining the metabolic dynamics of different lipid subtypes cells tissues are critical to better understanding their aging diseases. Commonly used imaging methods (such as mass spectrometry-based, fluorescence labeling, conventional optical imaging) can disrupt native environment cells/tissues, have limited or spectral resolution, cannot distinguish subtypes. Here we present a hyperspectral...
Significance Membranes can adopt distinct phases. The endoplasmic reticulum (ER) is the largest membrane system inside cells and also harbors richest metabolic activity including lipid synthesis. Unlike plasma where separated “lipid raft” domains have been predicted observed, ER thought to be uniformly fluidic. However, such understanding based on biophysical studies of model under thermodynamic equilibrium. It remains unclear whether how synthesis perturbs equilibrium promotes phase...
Significance Cells form structures and perform functions through intricate 3D tissue organizations. However, due to scattering, coherent Raman microscopy—a powerful method complementary fluorescence imaging—suffers from limited imaging depth in tissues. Here, we develop a volumetric chemical with greater than 10-fold increase. We formulate Raman-tailored tissue-clearing recipe combine it advanced microscopies. Equipped the toolbox of analyses, elucidate complex structures, compositions,...
Light scattering and transmission of Gaussian (G) Laguerre-Gaussian (LG) vortex beams with different orbital angular momentum (L) in various turbid media were investigated. Transmittance was measured varied ratios sample thickness (z) to mean free path (ls) media, z/ls. In the ballistic region, LG G found have no significant difference on transmittance, while diffusive showed a higher received signal than beams, L values those lower values. The transition points from regions for determined....
Stimulated Raman Scattering (SRS) nanoscopy imaging offers unprecedented insights into tissue molecular architecture but often requires stitching multiple high-resolution tiles to capture large fields of view. This process is time-consuming and frequently introduces vignetting artifacts—grid-like intensity fluctuations that degrade image quality hinder downstream quantitative analyses processing such as super-resolution deconvolution. We present VIgnetted Stitched-Tile Adjustment using...
Development of an optimal systemic drug delivery strategy to the brain will require noninvasive or minimally invasive methods quantify permeability cerebral microvessel wall blood-brain barrier (BBB) various therapeutic agents and measure their transport in tissue. To address this problem, we used laser-scanning multiphoton microscopy determine BBB solutes (P) effective solute diffusion coefficients (Deff) rat tissue 100–250 μm below pia mater. The microcirculation was observed through a...
There are three short wavelength infrared (SWIR) optical windows outside the conventionally used first near-infrared (NIR) window (650 to 950 nm). They occur in 1000- 2500-nm range and may be considered second, third, fourth NIR windows. The second (1100 1350 nm) third (1600 1870 now being explored through label-free linear multiphoton imaging. (2100 2350 has been mostly ignored because of water absorption absence sensitive detectors ultrafast lasers. With advent new technology, use IV is...
Glioma is one of the most refractory types brain tumor. Accurate tumor boundary identification and complete resection are essential for glioma removal during surgery. We present a method based on visible resonance Raman (VRR) spectroscopy to identify margins grades. A set diagnostic spectral biomarkers features presented tissue composition changes revealed by VRR. The spectra include molecular vibrational fingerprints carotenoids, tryptophan, amide I/II/III, proteins, lipids. These basic...
A two-color vibrational imaging technique for simultaneously mapping glucose uptake and incorporation activity inside living cells is reported.
Emerging studies have shown that lipids and proteins play versatile roles in various aspects of aging. High-resolution situ optical imaging provides a powerful approach to study the metabolic dynamics during Here, we integrated D2O probing stimulated Raman scattering (DO-SRS) microscopy directly visualize changes aging Drosophila ovary. The subcellular spatial distribution de novo protein synthesis lipogenesis ovary was quantitatively imaged examined. Our spectra showed early stages...
Light transmission of Laguerre‐Gaussian vector vortex beams in different local regions mouse brain tissue is investigated. Transmittance measured the ballistic and diffusive with various polarizations states orbital angular momentums (OAM). The change observed structured light other than linear polarization attributed to chiroptical phenomena from chiral media handedness light. For instance, classically entangled showed higher transmittance constant value dependency on OAM modes did. Also,...