A5061208000- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Parasites and Host Interactions
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Drug Transport and Resistance Mechanisms
- Chromatin Remodeling and Cancer
- Pancreatic and Hepatic Oncology Research
- Cancer-related gene regulation
- Phytase and its Applications
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA modifications and cancer
- Garlic and Onion Studies
- Nanocluster Synthesis and Applications
- Helminth infection and control
- Hops Chemistry and Applications
- Parasite Biology and Host Interactions
- Trace Elements in Health
- Biochemical Analysis and Sensing Techniques
- Ferroptosis and cancer prognosis
- Cancer Mechanisms and Therapy
- Cannabis and Cannabinoid Research
- Genomics, phytochemicals, and oxidative stress
Khon Kaen University
2015-2024
Faculty (United Kingdom)
2019
Mie University
2008-2013
Suzuka University of Medical Science
2011-2013
Institute of Cytochemistry and Molecular Pharmacology
2008
Abstract Cholangiocarcinoma (CCA) is a hepatobiliary malignancy exhibiting high incidence in countries with endemic liver-fluke infection. We analyzed 489 CCAs from 10 countries, combining whole-genome (71 cases), targeted/exome, copy-number, gene expression, and DNA methylation information. Integrative clustering defined 4 CCA clusters—fluke-positive (clusters 1/2) are enriched ERBB2 amplifications TP53 mutations; conversely, fluke-negative 3/4) exhibit copy-number alterations PD-1/PD-L2 or...
Early B cell factor 1 (EBF1) is a transcription involved in the differentiation of several stem lineages and it negative regulator estrogen receptors. EBF1 down-regulated many tumors, believed to play suppressive roles cancer promotion progression. However, functional carcinogenesis are unclear. Liver fluke-infection-associated cholangiocarcinoma (CCA) an oxidative stress-driven bile duct epithelium. In this study, we investigated expression tissues from CCA patients, lines (KKU-213, KKU-214...
Cholangiocarcinoma (CCA) is often diagnosed late, leading to incomplete tumor removal, drug resistance and reduced chemotherapy efficacy. Curcumin has the potential for anti-cancer activity through various therapeutic properties can improve efficacy of chemotherapy. We aimed investigate synergistic effect a combination curcumin gemcitabine against CCA, targeting LAT2/glutamine pathway. This synergistically suppressed proliferation in gemcitabine-resistant CCA cells (KKU-213B
Abstract Parasite infection of Opisthorchis viverrini is a major risk factor for cholangiocarcinoma. Our previous immunohistochemical studies showed that O. induced oxidative DNA lesions in the bile duct epithelium during cholangiocarcinoma development. The current study assessed levels 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), an lesion, urine and leukocytes viverrini–infected subjects patients. Forty-nine patients, 55 17 healthy controls were enrolled study. We measured 8-oxodG these...
Inflammation may activate stem cells via prostaglandin E2 (PGE2) production mediated by cyclooxygenase-2 (COX-2) expression. We performed an immunohistochemical analysis of the expression stemness markers (Oct3/4 and CD44v6) COX-2 in urinary bladder tissues obtained from cystitis cancer patients with without Schistosoma haematobium infections. Immunoreactivity to Oct3/4 was significantly higher S. -associated than normal tissues. CD44v6 located cytoplasmic membrane, cytoplasm, nucleus cells....
Background Chronic monosodium glutamate (MSG) intake causes kidney dysfunction and renal oxidative stress in the animal model. To gain insight into changes induced by MSG, proteomic analysis of kidneys was performed. Methods Six week old male Wistar rats were given drinking water with or without MSG (2 mg/g body weight, n = 10 per group) for 9 months. Kidneys removed, frozen, stored at –75°C. After protein extraction, 2-D gel electrophoresis performed proteome profiles examined Colloidal...
Epithelial-mesenchymal transition (EMT) is characterized by the loss of epithelial markers and gain mesenchymal markers. EMT believed to be a major mechanism supporting cancer cell metastasis. The activation can induced various types inflammatory cytokines including transforming growth factor β (TGF-β) whereas bone morphogenetic protein-7 (BMP-7) inhibit this process. In study, up-regulation Twist transcription N-cadherin, marker in CCA tissues, has been demonstrated it found that high...
Labile iron pool is a cellular source of ions available for Fenton reactions resulting in oxidative stress. Living organisms avoid an excess free irons by tight control homeostasis. We investigated the altered expression regulatory proteins and discrimination development liver fluke–associated cholangiocarcinoma. Additionally, levels labile functions transferrin receptor-1 on cholangiocarcinoma were also identified. Iron deposition was determined using Prussian blue staining method human...
Tumor interstitial fluid contains tumor-specific proteins that may be useful biomarkers for cancers. In this study, we identified present in cholangiocarcinoma fluid. Proteins derived from three samples of tumor and paired adjacent normal patients were subjected to two-dimensional liquid chromatography with tandem mass spectrometry. Candidate selected based on a greater than twofold change expression levels between Upregulation six fluid, including S100 calcium binding protein A6 (S100A6),...
The short- and long-term consumption of monosodium glutamate (MSG) increases urinary pH but the effects on metabolic pathways in liver, kidney gut microbiota remain unknown. To address this issue, we investigated adult male Wistar rats allocated to receive drinking water with or without 1 g% MSG for 2 weeks (n = 10, each). We performed a Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomic study jejunum, kidneys, while faecal samples were collected bacterial DNA extraction...
DNA methylation occurs when a methyl group is added to cytosine (C) residue's fifth carbon atom, forming 5-methylcytosine (5-mC). Cancer genomes have distinct landscape (Methylscape), which could be used as universal cancer biomarker. This study developed simple, low-cost, and straightforward Methylscape sensing platform using cysteamine-decorated gold nanoparticles (Cyst/AuNPs), in the principle based on methylation-dependent solvation. Normal DNAs profiles; thus, they can distinguished by...
DNA methylation is an epigenetic alteration that results in 5-methylcytosine (5-mC) through the addition of a methyl group to fifth carbon cytosine (C) residue. The level, ratio 5-mC C, urine might be related whole-body status and occurrence common cancers. To date, never before have any nanomaterials been developed simultaneously determine C samples. Herein, dual-responsive fluorescent sensor for urinary detection has developed. This assay relied on changes optical properties nitrogen-doped...
Cholangiocarcinoma (CCA) is usually diagnosed at a late stage, leading to treatment failure. Cannabidiol (CBD), exhibits diverse anti-cancer effects in various cancers, offering avenues for improving CCA treatment. This study investigated the of CBD on human cells and underlying mechanisms vitro vivo. The three cell lines (KKU-213B, KKU-100, KKU-055) were assessed using SRB assay, clonogenic extent cell-cycle arrest, 3D holotomography. Morphological changes examined transmission electron...
Cholangiocarcinoma (CCA) is one of the oxidative stress-driven carcinogenesis through chronic inflammation. Insulin receptor substrate 1 (IRS1), an adaptor protein insulin signaling pathways, associated with progression many inflammation-related cancers. This study hypothesized that stress regulates IRS1 expression and up-regulation induces CCA progression. The localizations marker (8-oxodG) were detected in tissues using immunohistochemistry (IHC). presence was confirmed immortal...
Plant-derived anti-cancer agents have been of considerable interest due to their promising effectiveness with low side effects. Asiatic acid, the main constituent medicinal plant Centella asiatica (L.) Urban, has a wide range biological properties such as antioxidant, anti-inflammatory and activities. Cholangiocarcinoma (CCA), which is malignant tumor bile duct epithelium, one leading cancers in Southeast Asia, notably northeast Thailand where liver fluke, Opisthorchis viverrini...
Cholangiocarcinoma (CCA) is a major health problem in northeastern Thailand. The majority of CCA cases are clinically silent and difficult to detect at an early stage. Although abdominal ultrasonography (US) can premalignant periductal fibrosis (PDF), this method not suitable for screening populations remote areas. With the goal developing blood test detecting at-risk population, we carried out serum protein biomarker discovery qualification. Label-free shotgun proteomics was performed on...
Background: Iron overload can lead to organ and cell injuries. Although the mechanisms of iron-induced damage have been extensively studied using various cells, little is known about these processes in kidney cells. Methods: In this study, we first examined correlation between serum iron levels function. Subsequently, investigated molecular impact excess on lines, HEK293T HK-2. The presence upregulated protein was further validated urine. Results: results revealed that caused significant...