A5002658824- Pancreatic and Hepatic Oncology Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatitis Pathology and Treatment
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Gallbladder and Bile Duct Disorders
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Neuroendocrine Tumor Research Advances
- Gastrointestinal disorders and treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Renal cell carcinoma treatment
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Liver Disease and Transplantation
- Circular RNAs in diseases
- Chromatin Remodeling and Cancer
- Cancer Genomics and Diagnostics
- Pediatric Hepatobiliary Diseases and Treatments
Duke-NUS Medical School
2015-2025
Singapore General Hospital
2015-2024
National Cancer Centre Singapore
2013-2024
National University of Singapore
2013-2023
SingHealth Duke-NUS Academic Medical Centre
2022
UNSW Sydney
2021
Singapore College of Traditional Chinese Medicine
2020
Sengkang General Hospital
2020
University of Birmingham
2020
Institute of Molecular and Cell Biology
2013
Abstract Cholangiocarcinoma (CCA) is a hepatobiliary malignancy exhibiting high incidence in countries with endemic liver-fluke infection. We analyzed 489 CCAs from 10 countries, combining whole-genome (71 cases), targeted/exome, copy-number, gene expression, and DNA methylation information. Integrative clustering defined 4 CCA clusters—fluke-positive (clusters 1/2) are enriched ERBB2 amplifications TP53 mutations; conversely, fluke-negative 3/4) exhibit copy-number alterations PD-1/PD-L2 or...
To compare microvascular invasion (McVI) with parameters defined by the Milan criteria in predicting tumor recurrence and overall survival (OS) patients surgical resection (SR) for hepatocellular carcinoma (HCC).Although is discriminatory selecting good outcomes liver transplantation SR HCC, it neither adequately predict nor explain differences function. McVI a strong indicator of intrahepatic metastasis but its relative significance clinical compared to unclear.Patients undergoing curative...
Tumor recurrence and metastases are the major obstacles to improving prognosis of patients with hepatocellular carcinoma (HCC). To identify novel risk factors associated HCC metastases, we have established a panel recurrence-associated microRNAs (miRNAs) by comparing miRNA expression in recurrent nonrecurrent human tissue samples using microarrays (recurrence is defined as disease occurring within 2-year time point original treatment). Among panel, miR-216a/217 cluster was consistently...
Hepatocellular carcinoma (HCC) is a heterogeneous disease with poor prognosis and limited methods for predicting patient survival. The nature of the immune cells that infiltrate tumours known to impact clinical outcome. However, molecular events regulate this infiltration require further understanding. Here ability genes expressed in tumour microenvironment predict progression was investigated.Using quantitative PCR, expression 14 resected tissues from 57 Singaporean patients analysed....
<h3>Objectives</h3> Hepatocellular carcinoma (HCC) is the second leading cause of cancer mortality worldwide. Alterations in microtubule-associated proteins (MAPs) have been observed HCC. However, mechanisms underlying these alterations remain poorly understood. Our aim was to study roles MAP protein regulator cytokinesis 1 (PRC1) hepatocarcinogenesis and early HCC recurrence. <h3>Design</h3> PRC1 expression samples evaluated by microarray, immunoblotting immunohistochemistry analysis....
Foreign body perforation of the gastrointestinal (GI) tract has diverse clinical manifestations, and correct preoperative diagnosis is seldom made. We report our experience with use CT in fish bone GI seven patients. To knowledge, this series largest to date addressing role perforation.Clinical presentation radiography are unreliable tract. This limitation can be overcome CT, which accurate showing offending bone. The accuracy limited by observer dependence. A high index suspicion should...
To determine whether first-line treatment with percutaneous or surgical drainage of liver abscesses larger than 5 cm results in better clinical outcome.Pyogenic are currently treated by intravenous antibiotics and either (PD) (SD). Percutaneous techniques have been increasingly performed place open as treatment. This paradigm shift has fueled the drive for low-risk less-invasive procedures option being reserved failures. Yet there is a lack data to support over treatment.Over 3-year period,...
Abstract Purpose: To improve the clinical management of human hepatocellular carcinoma (HCC) by accurate identification, at diagnosis, patients risk recurrence after primary treatment for HCC. Experimental Design: Two clinicopathologic variables available vascular invasion and cirrhosis, together with molecular profiling using Affymetrix HG-U133A HG-U133B oligonucleotide probe arrays, were used to identify recurrent HCC disease. Results: presented clinically diagnosis cirrhosis showed a high...
The down-regulation of miR-214 has previously been observed in human hepatocellular carcinoma (HCC). Here, we demonstrated the is associated with cell invasion, stem-like traits and early recurrence HCC. Firstly, validated suppression HCC by real-time quantitative RT-PCR (qRT-PCR) 20 paired tumor non-tumor liver tissues patients 10 histologically normal from colorectal cancer metastases. Further qRT-PCR analysis 50 an independent cohort whom 29 recurrent disease (<2 years) 21 late that was...
Abstract Purpose: To assess the significance of Rac GTPase-activating protein 1 (RACGAP1) expression in identifying HBV-positive human hepatocellular carcinoma (HCC) patients who are at high risk for recurrent disease. Experimental Design: The prognostic RACGAP1 was compared with clinicopathologic parameters available diagnosis using multivariate and log-rank test. outcome recurrence between 35 41 without Kaplan–Meier analysis. RACGAP1-targeted molecules pathways were identified...
Hepatocellular carcinoma (HCC) is the fifth most common cancer with high mortality, due to late diagnosis and limited treatment options. Blood miRNAs, which circulate in a highly stable, cell-free form, show promise as novel potential biomarkers for early detection of HCC. Whole miRNome profiling was performed identify deregulated miRNAs between HCC normal healthy (NH) volunteers. These were validated an independent cohort HCC, NH chronic Hepatitis B (CHB) volunteers finally 3rd comprising...
MicroRNA-224 (miR-224) is one of the most commonly up-regulated microRNAs in hepatocellular carcinoma (HCC), which affects crucial cellular processes such as apoptosis and cell proliferation. In this study, we aim to elucidate molecular mechanism that leads overexpression miR-224 HCC. We examined transcript expression neighboring miR-452 genes on chromosome Xq28 tumor paired adjacent nontumorous tissues from 100 patients with HCC found coordinately its microRNA (miRNA) genes. This...
This study aims to validate a previously reported recurrence clinical risk score (CRS).Salvage transplantation after hepatocellular carcinoma (HCC) resection is limited patients who recur within Milan criteria (MC). Predicting patterns may guide treatment recommendations.An international, multicenter cohort of R0 resected HCC were categorized by MC status at presentation. CRS was calculated assigning 1 point each for initial disease beyond MC, multinodularity, and microvascular invasion....
This study aimed to determine preoperative predictors of early (<1 year) mortality from disease recurrence after liver resection (LR) for huge (≥10 cm) HCC, with special emphasis on the importance neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), and prognostic nutrition index (PNI).Between 2000 2013, 166 patients underwent LR HCC. Optimal cut-offs alpha fetoprotein (AFP), NLR, PLR, PNI were determined by plotting receiver operator curves (ROC) in predicting utilizing...
The aim of this study is to report an update the surgical experience at a single institution with these unusual tumors.Sixteen consecutive patients who underwent surgery for pathologically confirmed solid pseudopapillary neoplasm (SPPN) were retrospectively reviewed.Fifteen female and median age diagnosis was 30 years (range, 14-53 years). Abdominal back pain most common presenting symptoms. tumors appeared on cross-sectional imaging as cystic (n = 14) or 2) masses. tumor size 9.5 cm...