A5011139799- Cholangiocarcinoma and Gallbladder Cancer Studies
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Pediatric Hepatobiliary Diseases and Treatments
- Advanced Chemical Sensor Technologies
- Biochemical Analysis and Sensing Techniques
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- melanin and skin pigmentation
- Proteoglycans and glycosaminoglycans research
- Drug Transport and Resistance Mechanisms
- Biochemical and Molecular Research
- Gallbladder and Bile Duct Disorders
- Oral and gingival health research
- Sphingolipid Metabolism and Signaling
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Cancer Cells and Metastasis
Khon Kaen University
2016-2025
Kumamoto University
2013-2024
Center for Translational Molecular Medicine
2020
Abstract Increased glucose utilization is a feature of cancer cells to support cell survival, proliferation, and metastasis. An association between diabetes mellitus progression was previously demonstrated in cancers including cholangiocarcinoma (CCA). This study aimed determine the effects high on protein O-GlcNAcylation metastatic potentials CCA cells. Two pairs each parental low highly sublines were cultured normal (5.6 mM) or (25 media. The migration invasion abilities determined...
Abstract O-GlcNAcylation, an O-linked protein glycosylation with a single molecule of N-acetylglucosamine (GlcNAc), is reversibly controlled by O-GlcNAc transferase (OGT) and N-acetyl D-glucosaminidase (OGA). Aberrant O-GlcNAcylation contributes important role in initiation progression many human cancers. Elevation tumor tissues poor prognosis cholangiocarcinoma (CCA) patients have been reported. In this study, the promoting was further investigated CCA cell lines. Suppression using small...
Artocarpus lakoocha agglutinin (ALA), which specifically targets the Gal/GalNAc components of complex glycans, was isolated from seeds lakoocha. This study is first to explore role ALA in identifying aberrant designated ALA-binding glycans (ALAG), and its implications cholangiocarcinoma (CCA). ALA-histochemistry used evaluate ALAG expression liver fluke-induced CCA tissues hamsters (n = 60). Elevated observed hyperplastic ducts significantly increased tissues, while normal biliary epithelium...
O‐GlcNAcylation is an important biological process in regulating the function of many nucleocytoplasmic proteins cells. Enhancement was associated with cancer development and progression. Here, we demonstrated involvement melanoma metastasis. Using data from GEO database, found that its related enzymes, including glutamine fructose‐6‐phosphate amidotransferase (GFAT), O‐GlcNAc transferase (OGT), O‐GlcNAcase (OGA); were elevated metastatic compared primary tumors normal tissues. Functional...
Alteration of glycosylation in cancer cells leads to the expression tumor-associated glycans, which can be used as biomarkers for diagnosis and prognostic prediction diseases. In this study, we nano-LC-QToF identify serum N-glycan detection brain tumors. We observed an increase sialylated N-glycans a decrease fucosylated patients with glioblastoma (GBM) meningioma (MG) compared healthy individuals. GBM, combination increased (6_4_0_2 compound) decreased (4_4_1_0 was identified most...
glycoPATH integrates RNAseq transcriptomic and LC-MS/MS glycomic data, providing a platform to identify genes implicated in diseases involving N -glycan biosynthesis enabling the development of targeted therapeutics for these pathways.
Abstract BACKGROUND: The incidence of cholangiocarcinoma (CCA) is increasing globally. Currently, there no powerful marker for the diagnosis CCA, which has led to late and poor patient outcome. This study was designed establish a new monoclonal antibody (MoAb) detecting serum associated with CCA. METHODS: Pooled CCA tissue extracts were immunized germinal center nuclear protein (GANP)‐transgenic mice. antibody‐producing hybridomas prepared initially screened by using an indirect...
Mucin type O-glycosylation is a posttranslational modification of membrane and secretory proteins. Transferring N-acetylgalactosamine, the first sugar O-glycosylation, catalyzed by one 20 isoforms polypeptide N-acetylgalactosaminyltransferases (GALNTs). In this study, Vicia villosa lectin (VVL), that recognizes O-GalNAcylated glycans, was used to detect VVL-binding glycans (VBGs) in cholangiocarcinoma (CCA). The elevation VBGs tumor tissues liver fluke associated with CCA from hamsters...
Cholangiocarcinoma (CCA) is a deadly malignant tumor of the liver. It significant health problem in Thailand. The critical obstacles CCA diagnosis and treatment are high heterogeneity disease considerable resistance to treatment. Recent multi-omics studies revealed promising targets for treatment; however, limited models drug discovery available. This study aimed develop patient-derived xenograft (PDX) model as well PDX-derived cell lines future screening. From total 16 frozen tissues, 75%...
The short- and long-term consumption of monosodium glutamate (MSG) increases urinary pH but the effects on metabolic pathways in liver, kidney gut microbiota remain unknown. To address this issue, we investigated adult male Wistar rats allocated to receive drinking water with or without 1 g% MSG for 2 weeks (n = 10, each). We performed a Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomic study jejunum, kidneys, while faecal samples were collected bacterial DNA extraction...
Early and specific diagnosis is critical for treatment of cholangiocarcinoma ( CCA ). In this study, a carbohydrate antigen‐S27 CA ‐ S 27) monoclonal antibody (m A b) was established using pooled tissue‐extract as immunogen. The epitope recognized by 27‐m b new L ewis‐a e ) associated modification MUC 5 AC mucin. oybean agglutinin/ ‐S27‐m sandwich ELISA to determine 27 in serum successfully developed. High level detected patients could differentiate from those gastro‐intestinal cancers,...
O‐Glc NA cylation is a key post‐translational modification that modifies the functions of proteins. Associations between cylation, shorter survival cholangiocarcinoma ( CCA ) patients, and increased migration/invasion cell lines have been reported. However, specific cylated proteins OGP s) participate in promotion progression are poorly understood. s were isolated from human lines, KKU ‐213 ‐214, using click chemistry‐based enzymatic labeling system, identified LC ‐ MS / , searched against...
The leading cause of death in cancer patients is metastasis, for which an effective treatment still necessary. During cells aberrantly express several glycans that are correlated with poor patient outcome. This study was aimed toward exploring the effects O-GlcNAcylation on membranous N-glycans associated progression cholangiocarcinoma (CCA). Global CCA depleted using specific siRNA against O-GlcNAc transferase (OGT), transfers GlcNAc to acceptor proteins. Using HPLC-Chip/Time-of-Flight...
Cholangiocarcinoma (CCA) is a bile duct cancer with very poor prognosis. Currently, there no effective pharmacological treatment available for it. We showed that CCA ubiquitously relies on cyclin-dependent kinases 4 and 6 (CDK4/6) activity to proliferate. Primary tissues express high levels of cyclin D1 the specific marker CDK4/6 activity, phospho-RB Ser780. Treatment 15-CCA cell line collection by inhibitors leads reduced numbers cells in S-phase senescence most lines. found expression...
YKL-40, a chitinase-like glycoprotein, is expressed at high level in cancer patients. Its exact function unknown and the subject of current investigation. Here, we report correlation plasma YKL-40 levels with clinicopathological features cholangiocarcinoma (CCA), lethal bile duct cancer, particularly prevalent Northeastern Thailand. Statistical analysis concentrations 57 CCA patients 41 normal healthy subjects gave median value 169.5 ng/mL for compared 46.9 control (P < 0.0001). There was no...
[Purpose] This study was to examine the effects of 12 weeks Tai Chi (TC) exercise on antioxidant capacity, and DNA damage/repair in young females who did not perform regular physical exercise. [Subjects Methods] Ten female students from a Chinese university voluntarily participated this program. All them practiced 24-form simplified Chi, 5 times weekly, for weeks. Plasma levels superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), (GSH), hydroxyl radical inhibiting...