A5002642306- Cholangiocarcinoma and Gallbladder Cancer Studies
- Glycosylation and Glycoproteins Research
- Genomics, phytochemicals, and oxidative stress
- Bone Tissue Engineering Materials
- Dental materials and restorations
- MicroRNA in disease regulation
- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- Sphingolipid Metabolism and Signaling
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Carbohydrate Chemistry and Synthesis
- Natural product bioactivities and synthesis
- Gallbladder and Bile Duct Disorders
- Drug Transport and Resistance Mechanisms
- Piperaceae Chemical and Biological Studies
- Galectins and Cancer Biology
- Ginger and Zingiberaceae research
- Lysosomal Storage Disorders Research
- Phytochemistry and Biological Activities
- Boron Compounds in Chemistry
- Pediatric Hepatobiliary Diseases and Treatments
- Lipid Membrane Structure and Behavior
- Coenzyme Q10 studies and effects
- Moringa oleifera research and applications
Suranaree University of Technology
2012-2025
Khon Kaen University
2012-2021
MicroRNA, an endogenous noncoding RNA modulating gene expression, is a key molecule that by its dysregulation plays roles in inflammatory-driven carcinogenesis. This study aimed to investigate the role of oncomiR miR-21 and target, programmed cell death 4 (PDCD4) tumor growth metastasis liver fluke Opisthorchis viverrini-associated cholangiocarcinoma (CCA). The expression levels PDCD4 were analyzed using TaqMan miRNA assay immunohistochemistry tissues both O. viverrini plus...
Gymnema inodorum (Lour.) Decne. (G. inodorum) is widely used in Northern Thai cuisine as local vegetables and commercial herb tea products. In the present study, G. extract (GIE) was evaluated for its antioxidant anti-inflammatory effects LPS plus IFN-γ-induced RAW264.7 cells. Major compounds GIE were using GC-MS found 16 volatile presenting extract. exhibited activity by scavenging intracellular reactive oxygen species (ROS) production increasing superoxide dismutase 2 (SOD2) mRNA...
Background Changes in protein glycosylation have been reported various diseases, including cancer; however, the consequences of altered meningiomas remains undefined. We established two benign meningioma cell lines—SUT-MG12 and SUT-MG14, WHO grade I—and demonstrated glycan glycosyltransferase profiles mucin-type O-linked primary cells compared with malignant lines—HKBMM IOMM-Lee, III. were proposed. Methods Primary culture technique, morphological analysis, immunocytochemistry used to...
YKL-40, a chitinase-like glycoprotein, is expressed at high level in cancer patients. Its exact function unknown and the subject of current investigation. Here, we report correlation plasma YKL-40 levels with clinicopathological features cholangiocarcinoma (CCA), lethal bile duct cancer, particularly prevalent Northeastern Thailand. Statistical analysis concentrations 57 CCA patients 41 normal healthy subjects gave median value 169.5 ng/mL for compared 46.9 control (P < 0.0001). There was no...
Changes in protein glycosylation have been reported various types of cancer, including cholangiocarcinoma (CCA). Nanospray ionization‑linear ion trap mass spectrometry (NSI‑MSn) was used the present study to determine comparative structural glycomics N‑linked glycans serum patients with CCA compared healthy controls. A total 5 high‑mannose and 4 complex were detected. Mannose7‑N‑acetyl‑glucosamine2 most abundant structure among (control 12.12±2.54 vs. 9.27±2.66%), whereas NeuAc2H2N2M3N2...
Oroxylum indicum (L.) Kurz has been used as plant‐based food and herbal medicine in many Asian countries. The aim of the present study was to examine antioxidant anti‐inflammatory activities O. extract ( ) RAW264.7 cells activated by LPS plus IFN‐ γ . phytochemical compounds were identified GC‐MS LC‐MS/MS. Five flavonoids (luteolin, apigenin, baicalein, oroxylin A, quercetin) 27 volatile found presented activities, including reducing ability FRAP assay free radical scavenging activity DPPH...
Cholangiocarcinoma (CCA) is a highly metastatic tumor, and the majority of patients with CCA have short survival time because there are no available effective treatments. Hence, better understanding regarding metastasis may provide an opportunity to improve strategies for treatment. A comparison study between cells their parental approach uncover molecular mechanisms underlying process. In present study, lung cell line, KKU-214L5, was established by in vivo selection tail vein-injected mouse...
RING finger protein 43 (RNF43) is a ubiquitin E3 ligase that negatively regulates Wnt/β-catenin signalling. Mutation, inactivation and downregulation of RNF43 in cholangiocarcinoma (CCA) are associated with less favourable prognosis. Since the functional role CCA has not yet been demonstrated, present study aimed to assess effect its overexpression mediating suppression via signalling pathway inhibition. Accordingly, was overexpressed, various malignant phenotypic changes studied, including...
Alteration of mucin-type O-glycosylation is implicated in tumor progression and metastasis cholangiocarcinoma (CCA). Core 1 β1-3 Galactosyltransferase (C1GALT1) a primary enzyme that regulates the elongation core 1-derived O-glycans. Dysregulation C1GALT1 has been documented multiple cancers associated with aberrant cancer aggressiveness; however, expression its role CCA remains unknown. Our study demonstrated was downregulated tissues at both mRNA protein levels. The biological function...
Piperlongumine (PL) produces reactive oxygen species (ROS) and induces G2/M‑phase arrest in cholangiocarcinoma (CCA) cells via the JNK/ERK pathway. A differential response to PL was observed among all CCA cell lines However, underlying mechanisms have remained be fully elucidated. The aim of present study investigate molecular PL‑induced heme oxygenase‑1 (HO‑1) expression lines. anti‑proliferative action KKU‑100 KKU‑213A analyzed using sulforhodamine B assays. Reverse...
Objective To investigate the expression of glycosphingolipids in serum and tissue from patients with cholangiocarcinoma compared healthy controls. Methods Nanospray ionization-linear ion trap mass spectrometry (NSI-MS n ) was used to demonstrate comparative structural glycomics (n=15), controls (n = 15). GM2 tissues 60) evaluated by immunohistochemistry. Results Eleven were detected NSI-MS : CMH (ceramide monohexose), Lac-Cer (galactose (Gal)β1-4 glucose (Glc)β1-1'-ceramide), Gb3...
Cholangiocarcinoma (CCA) is an aggressive tumor of the biliary epithelium with poor survival that shows limited response to conventional chemotherapy. Increased expression glucosylceramide synthase (GCS) contributes drug resistance and progression various cancers; profiles GCS (UGCG) genes for glucocerebrosidases 1, 2, 3 (GBA1, GBA2, GBA3) were therefore studied in CCA. The biological functions cell proliferation cisplatin sensitivity CCA explored. was higher tissues than GBA1, GBA3....
Protein glycosylation is the most common posttranslational modification in mammalian cells. Aberrant protein has been reported various diseases, including cancer. We identified and quantified glycan structures of O-linked glycoprotein from cholangiocarcinoma (CCA) cell lines different histological types compared their profiles by nanospray ionization-linear ion trap mass spectrometry (NSI-<TEX>$MS^n$</TEX>). Five human CCA lines, K100, M055, M139, M213 M214 were characterized. The results...
Abstract Glucosylceramide (GlcCer) is a major membrane lipid and the precursor of gangliosides. It continuously formed degraded in glycosphingolipid metabolism. GlcCer mainly by two enzymes, lysosomal acid β-glucosidase (GBA) nonlysosomal (GBA2). Deficiencies GBA GBA2 affect metabolism, resulting neurological diseases, such as Gaucher Disease Hereditary Spastic Paraplegia. To understand which isoforms are active how they levels cells, we expressed nine human COS-7 confirmed their expression...
Abstract This study has investigated the effect of combination new liquid phase mixture disodium hydrogen phosphate (Na 2 HPO 4 ) and polyacrylic acid (PAA) on compressive strength, setting time, bioactivity, cytotoxicity biphasic calcium cement. The PAA was known as one water‐soluble biocompatible polymers to improve mechanical performances bone cement, but it usually inhibits conversion hydroxyapatite after cement already set. aim this work evaluate incorporation Na into We have found...