A5017070823- Cholangiocarcinoma and Gallbladder Cancer Studies
- Circular RNAs in diseases
- FOXO transcription factor regulation
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Cancer Mechanisms and Therapy
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Gene Regulatory Network Analysis
- Image Processing Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Parasites and Host Interactions
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Mycobacterium research and diagnosis
- Microbial infections and disease research
Roi et Rajabhat University
2023-2025
Siriraj Hospital
2019-2025
Mahidol University
2019-2025
Khon Kaen University
2013-2014
Background This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator. Methods MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified differential both tumor tissues (of Opisthorchis viverrini (Ov)-induced model) TaqMan miRNA expression assay. Results Our results demonstrated that...
MicroRNA, an endogenous noncoding RNA modulating gene expression, is a key molecule that by its dysregulation plays roles in inflammatory-driven carcinogenesis. This study aimed to investigate the role of oncomiR miR-21 and target, programmed cell death 4 (PDCD4) tumor growth metastasis liver fluke Opisthorchis viverrini-associated cholangiocarcinoma (CCA). The expression levels PDCD4 were analyzed using TaqMan miRNA assay immunohistochemistry tissues both O. viverrini plus...
ABSTRACT The cell cycle is crucial for maintaining normal cellular functions and preventing replication errors. FOXM1, a key transcription factor, plays pivotal role in regulating progression implicated various physiological pathological processes, including cancers like liver, prostate, breast, lung colon cancer. Despite previous research, our understanding of FOXM1 dynamics under different perturbations its connection to heterogeneous fate decisions remains limited. In this study, we...
Cholangiocarcinoma (CCA) is a bile duct cancer with very poor prognosis. Currently, there no effective pharmacological treatment available for it. We showed that CCA ubiquitously relies on cyclin-dependent kinases 4 and 6 (CDK4/6) activity to proliferate. Primary tissues express high levels of cyclin D1 the specific marker CDK4/6 activity, phospho-RB Ser780. Treatment 15-CCA cell line collection by inhibitors leads reduced numbers cells in S-phase senescence most lines. found expression...
Prostaglandin E2 (PGE2) involves in progression of various chronic inflammation-related cancers including cholangiocarcinoma (CCA). This study aimed to determine the role PGE2 signaling, its biosynthesis-related enzymes a clinical prognosis, and their targeted inhibition CCA progression. The immunohistochemical staining cyclooxygenase (COX)-1, COX-2, mPGES-1, EP1, EP4 was examined tissues, expressions were compared with clinicopathological parameters. effect on levels signaling molecules...
Abstract Forkhead box protein M1 (FOXM1) is a proliferation-associated transcription factor contributing to the G2/M phase transition of cell cycle. Although upregulation FOXM1 has been observed in different cancer types, how regulation dynamically alters during cycles and potentially contributes tumorigenesis not well understood. We showed here development application tunable FOXM1-DHFR (FOXM1-D) sensor that enables surveillance manipulation abundance. Using trimethoprim (TMP) stabilize...
This study investigates the use of advanced computer vision techniques for assessing severity Orientia tsutsugamushi bacterial infectivity. It uses fluorescent scrub typhus images obtained from molecular screening, and addresses challenges posed by a complex extensive image dataset, with limited computational resources. Our methodology integrates three key strategies within deep learning framework: transitioning instance segmentation (IS) models to an object detection model; reducing model's...
Abstract The cholangiocarcinoma cell-exposed cytokine-induced killer (CIK) cells or their most efficacious CD3+CD56+ subset displayed weaken anti-tumor cytotoxicity upon subsequent exposure. RNAseq analysis of the resistant phenotype gave rise to a list drugs that could potentially counter activity genes associated with resistance CIK cells. top-ranking appeared be epigenetic drugs. Sublethal concentration these were assayed for synergistic cell cytolysis CCA in tissue culture model. These...
Abstract The cell cycle plays a critical role in maintaining normal cellular functions and preventing abnormal growth. transcription factor FOXM1 has emerged as crucial regulator of progression been implicated various physiological pathological processes. Notably, overexpression observed numerous cancer types, including liver, prostate, breast, lung, colon cancer. Despite its significance, our understanding how dynamic behaves at the single-cell level under different perturbations link to...
ABSTRACT The cell cycle is crucial for maintaining normal cellular functions and preventing replication errors. FOXM1, a key transcription factor, plays pivotal role in regulating progression implicated various physiological pathological processes, including cancers like liver, prostate, breast, lung, colon cancer. Despite previous research, our understanding of FOXM1 dynamics under different perturbations its connection to heterogeneous fate decisions remains limited. In this study, we...
Acquiring fluorescent scrub typhus images obtained through RNA interference screening for the analysis of 60 different human genes and 18 control poses challenges due to nonuniform or clumped cells variations in image quality, rendering processing (IP) counting inadequate. This study addresses three key questions concerning application deep learning methods this dataset. Firstly, it explores potential object detection (OD) models replace instance segmentation (IS) cell counting, striking a...
Abstract Clinical trials on cholangiocarcinoma revealed differential sensitivity to cytolytic action of allogeneic cytokine-induced killer (CIK) cells. Different metastatic foci in the same individual could also display different sensitivity. The separate lines cultured cells be demonstrated based evaluation GR50. Allogeneic CIK cell or CD3+CD56+ subset was co-cultured with nine at E:T ratio 20:1 for 48 h. tumor were categorized into 2 groups obtained This resulted 4 sensitive and 5...