A5009807764- Cholangiocarcinoma and Gallbladder Cancer Studies
- Skin Protection and Aging
- FOXO transcription factor regulation
- melanin and skin pigmentation
- Cancer Mechanisms and Therapy
- Genomics, phytochemicals, and oxidative stress
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Cancer Immunotherapy and Biomarkers
- Magnetic properties of thin films
- Salivary Gland Tumors Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Rare-earth and actinide compounds
- Cancer Genomics and Diagnostics
- Advanced Nanomaterials in Catalysis
- Carbon and Quantum Dots Applications
- Microfluidic and Bio-sensing Technologies
- Heat shock proteins research
- RNA modifications and cancer
- Hippo pathway signaling and YAP/TAZ
- Pancreatic function and diabetes
- Electrochemical sensors and biosensors
- Magnetic Properties of Alloys
- Drug Transport and Resistance Mechanisms
- Ferroptosis and cancer prognosis
Siriraj Hospital
2016-2025
Mahidol University
2016-2025
Center for Systems Biology
2018
Harvard University
2018
Johns Hopkins University
2005
Johns Hopkins Medicine
2005
Abstract Embryonic stem (ES) cells have the ability to self-replicate and differentiate into from all three germ layers, holding great promise for tissue regeneration applications. However, controlling differentiation of ES obtaining homogenous cell populations still remains a challenge. We hypothesize that supportive three-dimensional (3D) environment provides cell-derived an more closely mimics chondrogenesis in vivo. In present study, chondrogenic capability embryoid bodies (EBs)...
Cholangiocarcinomas (CCA) pose a complex challenge in oncology due to diverse etiologies, necessitating tailored therapeutic approaches. This review discusses the risk factors, molecular pathology, and current options for CCA explores emerging strategies encompassing targeted therapies, immunotherapy, novel compounds from natural sources, modulation of gut microbiota. are driven by an intricate landscape genetic mutations, epigenetic dysregulation, post-transcriptional modification, which...
UVA irradiation plays a role in premature aging of the skin through triggering oxidative stress-associated stimulation matrix metalloproteinase-1 (MMP-1) responsible for collagen degradation, hallmark photoaged skin. Compounds that can activate nuclear factor E2-related 2 (Nrf2), transcription regulating antioxidant gene expression, should therefore serve as effective antiphotoaging agents. We investigated whether genetic silencing Nrf2 could relieve UVA-mediated MMP-1 upregulation via...
Responses of melanocytes (MC) to ultraviolet (UV) irradiation can be influenced by their neighbouring keratinocytes (KC). We investigated the role Nrf2 in regulating paracrine effects KC on UVB-induced MC responses through phosphorylation MAPKs association with oxidative stress primary human cocultured using a transwell co-culture system and small-interfering RNA-mediated silencing (siNrf2). The mechanisms which modulated factors including α-melanocyte-stimulating hormone (α-MSH)...
Colorectal cancers (CRC) with KRAS mutations (KRASmut) are frequently included in consensus molecular subtype 3 (CMS3) profound metabolic deregulation. We explored the transcriptomic impact of KRASmut, focusing on tumor microenvironment (TME) and pathways beyond The status KRASmut patients CRC was investigated overall survival (OS) compared wild-type (KRASwt). Next, we identified CMS, further differentially expressed genes (DEG) distinctive pathways. Lastly, used spatially resolved gene...
ABSTRACT The cell cycle is crucial for maintaining normal cellular functions and preventing replication errors. FOXM1, a key transcription factor, plays pivotal role in regulating progression implicated various physiological pathological processes, including cancers like liver, prostate, breast, lung colon cancer. Despite previous research, our understanding of FOXM1 dynamics under different perturbations its connection to heterogeneous fate decisions remains limited. In this study, we...
Application of machine learning (ML) on cancer-specific pharmacogenomic datasets shows immense promise for identifying predictive response biomarkers to enable personalized treatment. We introduce CAN-Scan, a precision oncology platform, which applies ML next-generation generated from freeze-viable biobank patient-derived primary cell lines (PDCs). These PDCs are screened against 84 Food and Drug Administration (FDA)-approved drugs at clinically relevant doses (Cmax), focusing colorectal...
Abstract This study set forth to investigate the efficacy of Quality and Quantity mononuclear cells (QQMNCs) for promoting wound healing limb salvage in a severe ischemic model using diabetic mice. Female BALB/c nude mice induced with diabetes were used create models controlled experimental design. Intramuscular injections human QQMNCs compared phosphate‐buffered saline (PBS) peripheral blood (PBMNCs) relative their effects on salvage. In vitro analysis demonstrated that QQMNC group had...
Summary Bacterial cell walls are composed of the large cross‐linked macromolecule peptidoglycan, which maintains shape and is responsible for resisting osmotic stresses. This a highly conserved structure target numerous antibiotics. Obligate intracellular bacteria an unusual group organisms that have evolved to replicate exclusively within cytoplasm or vacuole eukaryotic cell. They tend reduced amounts likely due fact their growth division takes place osmotically protected environment, also...
Expression of cyclin D1 is required for cancer cell survival and proliferation. This presumably due to the role in RB inactivation. Here we investigated prosurvival function a number lines. We found that depletion facilitated cellular senescence several lines tested. Senescence triggered by was more extensive than caused prolonged CDK4 inhibition. Intriguingly, independent status cell. identified buildup intracellular reactive oxygen species, cells underwent upon depletion, but not...
Background/Aim: Multiple myeloma (MM), the second most common hematological malignancy, is characterized by accumulation of malignant plasma cells within bone marrow. Despite various drug classes for MM treatment, it remains incurable, necessitating novel and efficacious agents. This study aims to explore anti-cancer activity a midkine inhibitor, iMDK (C<sub>21</sub>H<sub>13</sub>FN<sub>2</sub>O<sub>2</sub>S), in cell lines. Materials Methods: assessed antiproliferative using MTT assay. Cell...
Cholangiocarcinoma (CCA) is a bile duct cancer with very poor prognosis. Currently, there no effective pharmacological treatment available for it. We showed that CCA ubiquitously relies on cyclin-dependent kinases 4 and 6 (CDK4/6) activity to proliferate. Primary tissues express high levels of cyclin D1 the specific marker CDK4/6 activity, phospho-RB Ser780. Treatment 15-CCA cell line collection by inhibitors leads reduced numbers cells in S-phase senescence most lines. found expression...
Cholangiocarcinoma (CCA) is one of the most difficult to treat cancers, and its nature being largely refractory most, if not all, current treatments results in generally poor prognosis high mortality. Efficacious alternative therapies that can be used ubiquitously are urgently needed. Using acquired vulnerability screening, we observed CCA cells reprofile proliferate under CDK4/6 inhibition became vulnerable ribosomal biogenesis stress hypersensitive anti-ribosome chemotherapy oxaliplatin....
Oxidative damage has been suggested to play a role in the pathogenesis of basal cell carcinoma (BCC). This study illustrated an involvement oxidative DNA and changes antioxidant defenses BCC by conducting case‐control (24 controls 24 patients) assessing urinary 7,8‐dihydro‐8‐oxo‐2′‐deoxyguanosine (8‐oxo‐dGuo), plasma including catalase (CAT), glutathione peroxidase (GPx), NQO1, total superoxide dismutase (SOD) activities, (GSH) levels before surgery 1 month after surgery. 8‐oxo‐dGuo...
Cholangiocarcinoma (CCA) is a highly lethal gastrointestinal malignancy that has one of the worst prognoses among solid tumors. The combination Gemcitabine + Cisplatin (GEM/CIS) remains standard first-line treatment for advanced stage CCA. However, this drug yields only modest objective response rate, and in cases initially respond to treatment, resistance commonly rapidly develops. To improve efficiency GEM/CIS therapy CCA, thorough understanding mechanism CCA required. end – study, we...
The FoxO3 transcription factor is a key regulator of oxidative stress and erythroid maturation during erythropoiesis. In this study, we explored the involvement in severe β-thalassemia. Using primary CD34+ hematopoietic progenitor cells from patients with β-thalassemia major, successfully developed an vitro model ineffective Based on model, activity was quantified single using high throughput imaging flow cytometry. This study revealed significant reduction late stage erythroblast...
Cancer cells adeptly manipulate the tumor microenvironment (TME) to evade host antitumor immunity. However, role of cancer cell-intrinsic signaling in shaping immunosuppressive TME remains unclear. Here, we found that Hippo pathway orchestrates by influencing composition cancer-associated fibroblasts (CAFs). In a 4T1 mouse breast model, kinases, large suppressor 1 and 2 (LATS1/2), promoted formation neural cell adhesion molecule (NCAM1)
Cholangiocarcinoma (CCA) is rare cancer with the highest incidence in Eastern and Southeast Asian countries. Advanced CCA patients rely on chemotherapeutic regimens that offer unsatisfied clinical outcomes. We developed a comprehensive drug response profiling to investigate potential new drugs using cell lines from Thai Japanese against 100 approved anti-cancer drugs. identified two major subgroups displayed unique molecular pathways our integrative pan-omic ligand-induced pathway activation...
Abstract Wound healing assay is a simple and cost-effective in vitro for assessing therapeutic impacts on cell migration. Its key limitation the possible confoundment by other cellular phenotypes, causing misinterpretation of experimental outcome. In this study, we attempted to address problem developing analytical approach scoring influences both migration death, while normalizing influence growth using Mitomycin C pre-treatment. By carefully mapping relationship between death wound closure...
Carbon dots are fascinating nanomaterials due to their low cost, straightforward preparation, unique optical properties, and biocompatibility. In this work, carbon were prepared from yogurt using pyrolysis method. The characterized UV-visible spectroscopy, fluorescence Fourier transform infrared transmission electron microscopy. applied for detecting formic acid vapor electronic nose system. Furthermore, the biocompatibility of investigated show potentials biomedical applications. Based on...