A5053646531- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- PI3K/AKT/mTOR signaling in cancer
- Medical and Biological Sciences
- Mathematical Biology Tumor Growth
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Fibroblast Growth Factor Research
- Reproductive tract infections research
- Genomics and Rare Diseases
- Ethics in Clinical Research
- Viral Infections and Vectors
- Cellular Mechanics and Interactions
- BRCA gene mutations in cancer
- Endometrial and Cervical Cancer Treatments
- HIV Research and Treatment
- Genomics, phytochemicals, and oxidative stress
- MRI in cancer diagnosis
- Gene Regulatory Network Analysis
- Melanoma and MAPK Pathways
- Pluripotent Stem Cells Research
- Cell Image Analysis Techniques
- Autophagy in Disease and Therapy
University of California, Davis
2017-2025
Mahidol University
2018
Siriraj Hospital
2017-2018
Cells use multiple feedback controls to regulate metabolism in response nutrient and signaling inputs. However, creates the potential for unstable network responses. We examined how concentrations of key metabolites pathways interact maintain homeostasis proliferating human cells, using fluorescent reporters AMPK activity, Akt cytosolic NADH/NAD+ redox. Across various conditions, including glycolytic or mitochondrial inhibition cell proliferation, we observed distinct patterns both stable...
Summary Bacterial cell walls are composed of the large cross‐linked macromolecule peptidoglycan, which maintains shape and is responsible for resisting osmotic stresses. This a highly conserved structure target numerous antibiotics. Obligate intracellular bacteria an unusual group organisms that have evolved to replicate exclusively within cytoplasm or vacuole eukaryotic cell. They tend reduced amounts likely due fact their growth division takes place osmotically protected environment, also...
mTORC1 senses nutrients and growth factors phosphorylates downstream targets, including the transcription factor TFEB, to coordinate metabolic supply demand. These functions position as a central controller of cellular homeostasis, but behavior this system in individual cells has not been well characterized. Here, we provide measurements necessary refine quantitative models for controller. We developed series fluorescent protein-TFEB fusions multiplexed immunofluorescence approach...
Since the hemorrhage in severe dengue seems to be primarily related defect of platelet, possibility that virus (DENV) is selectively tropic for one its surface receptors was investigated. Flow cytometric data DENV-infected megakaryocytic cell line superficially expressing human glycoprotein Ib (CD42b) and IIb/IIIa (CD41 CD41a) were analyzed by our custom-written software MATLAB. In two-dimensional analyses, intracellular DENV detected CD42b
The Ras/ERK pathway drives cell proliferation and other oncogenic behaviors, quantifying its activity
The aim of this study was to investigate the predictive value radiomic features pre-treatment T2-weighted magnetic resonance images (MRI) for clinical outcomes radiotherapy in cervical cancer patients.
Abstract Cells use multiple feedback controls to regulate metabolism in response nutrient and signaling inputs. However, creates the potential for unstable network responses. We examined how concentrations of key metabolites pathways interact maintain homeostasis proliferating human cells, using fluorescent reporters AMPK activity, Akt cytosolic NADH/NAD + redox. Across various conditions including metabolic (glycolytic or mitochondrial) inhibition cell proliferation, we observed distinct...
Abstract Cellular metabolism can be reconfigured to balance nutrient processing (catabolism) and cellular demands (anabolism). However, the kinetics of reconfiguration within individual mammalian cells, heterogeneity between have remained unexplored. Using live-cell imaging, we investigate bioenergetic adaptation in cells. In response acute inhibition oxidative phosphorylation, AMPK substrates are phosphorylated bimodally, identifying cells different underlying states anabolism catabolism....
Abstract mTORC1 senses nutrient and growth factor status phosphorylates downstream targets, including the transcription TFEB, to coordinate metabolic supply demand. The molecular mechanisms of activation are thought enforce a strict requirement for simultaneous amino acid stimuli, but this model has not been evaluated with quantitative or single-cell methods. Here, we develop series fluorescent protein-TFEB fusions investigate how combinations stimuli jointly regulate signaling from TFEB at...